5μL generally tends to show a higher volume and less precision than the remaining 4 plates in the dispense sequence. This effect is believed to be due to any residual tartrazine solution on the external surfaces of the cannula. These contributing factors can create a noticeable effect due to the higher concentration of the tartrazine solution for the lowest dispensing volumes. However, taking the result of the first plate into account, these results are still within in the effective working range for most applications.

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