Monitoring fluorescence resonance energy transfer (FRET) between GFP,,, fusions in lysates of the yeast Saccharomyces cerevisiae using the ,,, Varian,,, Cary Eclipse
Monitoring fluorescence resonance energy transfer (FRET) between GFP
fusions in lysates of the yeast Saccharomyces cerevisiae using the
Paul Gavin# and Mark Prescott#, Ph.D
Daren J. Fyfe, Ph.D*
# Department of Biochemistry and Molecular Biology, Monash University,
Clayton campus Victoria 3800, Australia
* Technical assistance: Varian Australia Pty Ltd, Mulgrave, Victoria 3170,
Australia E-mail: firstname.lastname@example.org
Fluorescence resonance energy transfer (FRET) is a non-destructive, spectroscopic
approach that can be used to monitor the proximity and angular orientation of
donor and acceptor fluorophores in living cells1. The resonant energy of an
excited donor fluorophore (in this example blue fluorescent proteinBFP) is
absorbed by an acceptor fluorophore (green fluorescent proteinGFP) providing
that donor and acceptor are in close proximity (between 1080 ngstroms2) (Figure
1). Emission spectra of donor fluorophores must significantly overlap the absorption
spectra of the acceptor, while overlap between respective absorption and emission
spectra of donor and acceptor should be minimized 3 .
Due to the attractive attributes of GFP (previously described in fluorescence
application note No. 5)4, the application of FRET to GFP and GFP variants has
become a powerful tool to monitor interactions at the protein level, within
an intact cell or organism. It is possible to use FRET to measure conformational
changes in a molecule tagged with two GFP variants in response to the binding
of ligands such as calcium1, or the interaction betwPage: All 1 2 3 4 Related biology technology :1
. Versatile Reporter Vectors for Monitoring Viral Transduction2
. Monitoring transfection efficiency by
green fluorescent protein (GFP) detection
with the Agilent 2100 bioanalyzer3
. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System4
. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis5
. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS6
. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation7
. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays8
. Immunoassay of Theophylline by fluorescence polarisation9
. Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP10
. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives
in the yeast Saccharomyces cerevisiae: Detection in vivo using
the Varian Cary Eclipse11
. Saccharomyces cerevisiae
(Date:10/1/2014)... 01, 2014 TeselaGen Biotechnology ... Foundation to expand their bioCAD/CAM software suite, which uses ... DNA. The Small Business Innovation Research Phase II grant ... under exclusive license from the Lawrence Berkeley National Laboratory. ... , an LBNL laboratory that develops alternative fuel technologies ...
(Date:9/30/2014)... September 30, 2014 Applying a well-known optical ... at Harvard University has demonstrated the ability to ... work that holds promise for making future, flexible ... the sides of next-gen rocket ships and spacecraft ... passes through oil in water, it becomes iridescent, ...
(Date:9/30/2014)... Shimadzu Scientific Instruments has released ... highly precise tool for measuring displacement in rubber ... 1000-mm maximum movement distance, the DSES-1000 can measure ... length of 10 mm. In addition, it achieves ... stroke and within +/- 100 µm below 50-mm ...
(Date:9/30/2014)... Sept. 30, 2014 RegeneRx Biopharmaceuticals, Inc. (OTC: ... it has received a Canadian Patent for Inhibition ... This patent includes claims using thymosin beta 4, ... peptides for these purposes. The patent will expire ... Inc. ( www.regenerx.com ) RegeneRx ... Breaking Biology Technology:TeselaGen Receives $750k NSF SBIR Phase II Award 2TeselaGen Receives $750k NSF SBIR Phase II Award 3Taking thin films to the extreme 2Shimadzu’s New Long-Travel Extensometer Increases Precision for Soft Material Testing 2RegeneRx Receives Canadian Patent for Inhibition or Reversal of Skin Aging 2
... Six new stem cell therapies, all forecast to reach physicians ... Summit on February 16 in New York City. These new therapies ... at least one FDA-approved stem cell drug. New studies showing rehydration ... are also on the agenda. , The Stem Cell Summit is ...
... REHOVOT, Israel, December 31 D-Pharm Ltd ... its,Phase III clinical study of DP-b99 (MACSI). The first patient ... trial involves,numerous medical centers in the US, Canada, Europe, Israel, ... product developed,for protection of brain cells suffering from restricted blood ...
... ... For The Massachusetts Biotechnology Council , ... Boston, MA (PRWEB) December 31, 2009 -- Social Media ... Biotechnology Council’s (MassBio) has selected the Boston-based Social Media Marketing Agency to plan and ...Cached Biology Technology:5th Annual Summit to Feature New Stem Cell Therapies 2D-Pharm (TASE: DPRM) Announces Enrollment of First Patient in DP-b99 Phase III Efficacy Study, MACSI 2D-Pharm (TASE: DPRM) Announces Enrollment of First Patient in DP-b99 Phase III Efficacy Study, MACSI 3 The Massachusetts Biotechnology Council Selects Social Media Marketing Agency 451 Marketing To Develop A Social Media Strategy For 2010 2 The Massachusetts Biotechnology Council Selects Social Media Marketing Agency 451 Marketing To Develop A Social Media Strategy For 2010 3
(Date:9/30/2014)... the Mediterranean each year, but its deep-blue waters ... Almost 1,000 alien species, including fish, crustaceans, and ... human activities. In the open-access journal Frontiers ... researchers analyzed data from a new information system ... the introduction of alien species has changed the ...
(Date:9/30/2014)... --- Do we think of nature as something that we ... need to "preserve?" Or do we think of ourselves as ... of nature, but what about a house? , The ... also reflected in our actions, our speech and in cultural ... the University of Washington, the American Indian Center of Chicago ...
(Date:9/30/2014)... 2014--Sea monkeys have captured the popular attention of both ... life cycle -- sold as dehydrated eggs, these tiny ... more than a tank of salt water. , ... other zooplankton, brine shrimp vertically migrate in large groups ... the surface at night and retreating deeper during the ... Breaking Biology News(10 mins):Biodiversity in the Mediterranean is threatened by alien species 2The cultural side of science communication 2Laser-guided sea monkeys show how zooplankton migrations may affect global ocean currents 2
... After a decade and a half of near stagnation, ... chronic illness instead of a terminal disease, a leading Colorado ... drugs have different and innovative methods of action. One is ... another is radiotherapy; and the final one is the first ...
... ingredient in the food industry because of its association with ... the February issue of the Journal of Food Science ... fish oil can be added to goat cheese to deliver ... or shelf-life. Fish oil delivers higher levels and more ...
... Republic of Congo has formally expanded Nouabal-Ndoki National Park ... most pristine forests and a population of "nave" chimpanzees ... apes investigate the conservationists who study them rather than ... 100-plus square-mile dense swamp forest and its unique great ...Cached Biology News:4 new drugs will change prostate cancer care 2Great news for chimpanzees 2Great news for chimpanzees 3
... Signal Enhancer does for enzyme-/substrate-based blotting what intensifying ... the signal up to 10-fold (or one order ... Western Blot Signal Enhancer membrane treatment is a ... to your current Western blotting protocol. The result ...
... revolutionary new application for DNA and ... interface, Expression makes complex computational analyses ... uses the very latest computing technology ... way sequences are analysed. Features include: ...
... Partek Genomics Solution is a ... interactive data visualization specifically designed to ... Designed for high-dimensional genomic studies containing ... is fast, memory efficient and will ...Biology Products: