Navigation Links
Monitoring fluorescence resonance energy transfer (FRET) between GFP,,, fusions in lysates of the yeast Saccharomyces cerevisiae using the ,,, Varian,,, Cary Eclipse

Monitoring fluorescence resonance energy transfer (FRET) between GFP
fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian
Cary Eclipse

Paul Gavin# and Mark Prescott#, Ph.D

Daren J. Fyfe, Ph.D*

# Department of Biochemistry and Molecular Biology, Monash University, Clayton campus Victoria 3800, Australia

* Technical assistance: Varian Australia Pty Ltd, Mulgrave, Victoria 3170, Australia E-mail: fluorescence@varianinc.com

Introduction

Fluorescence resonance energy transfer (FRET) is a non-destructive, spectroscopic approach that can be used to monitor the proximity and angular orientation of donor and acceptor fluorophores in living cells1. The resonant energy of an excited donor fluorophore (in this example blue fluorescent proteinBFP) is absorbed by an acceptor fluorophore (green fluorescent proteinGFP) providing that donor and acceptor are in close proximity (between 1080 ngstroms2) (Figure 1). Emission spectra of donor fluorophores must significantly overlap the absorption spectra of the acceptor, while overlap between respective absorption and emission spectra of donor and acceptor should be minimized 3 .

Due to the attractive attributes of GFP (previously described in fluorescence application note No. 5)4, the application of FRET to GFP and GFP variants has become a powerful tool to monitor interactions at the protein level, within an intact cell or organism. It is possible to use FRET to measure conformational changes in a molecule tagged with two GFP variants in response to the binding of ligands such as calcium1, or the interaction betw
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
5. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
6. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation
7. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
8. Immunoassay of Theophylline by fluorescence polarisation
9. Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP
10. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
11. Saccharomyces cerevisiae
Post Your Comments:
(Date:3/29/2015)... 2015  Caris Life Sciences® today announced the ... Intelligence®, the company,s panomic, comprehensive tumor profiling service, ... rare and difficult to treat gynecological cancers may ... to improve patient outcomes. These studies were presented ... of Gynecologic Oncology (SGO) 2015 Annual Meeting on ...
(Date:3/29/2015)... March 29, 2015  Caris Life Sciences® ... in small cell cervical cancer (SCCC) that ... Intelligence®, the company,s panomic, comprehensive tumor profiling ... which may explain sensitivities and resistance to ... of certain biomarkers may guide treatment strategies ...
(Date:3/29/2015)... Texas , March 29, 2015 /PRNewswire/ ... presentation of important study data showing the ... in 1,599 gynecologic cancer samples. Using Caris ... profiling service, researchers identified expression of programmed ... cancers independent of histology while its ligand ...
(Date:3/29/2015)... NJ (PRWEB) March 29, 2015 As ... deadlines often required by our pharmaceutical manufacturing clients, Whitehouse ... its sponsorship of the Berkenstock Race Team for the ... requires not only speed, but a strong sense of ... principles that lead to success in the quality control ...
Breaking Biology Technology:Caris Molecular Intelligence Facilitates Comparison of Biomarker Profiles to Inform Targeted Treatment Strategies in Distinct Gynecological Cancers 2Caris Molecular Intelligence Facilitates Comparison of Biomarker Profiles to Inform Targeted Treatment Strategies in Distinct Gynecological Cancers 3Caris Molecular Intelligence Facilitates Comparison of Biomarker Profiles to Inform Targeted Treatment Strategies in Distinct Gynecological Cancers 4Study Using Caris Molecular Intelligence Multiplatform Tumor Profiling Identifies Potential Treatment Strategies for Small Cell Cervical Cancer 2Study Using Caris Molecular Intelligence Multiplatform Tumor Profiling Identifies Potential Treatment Strategies for Small Cell Cervical Cancer 3Caris Life Sciences' Multiplatform Tumor Profiling Technology Identifies Expression of PD-1 and PD-L1 in Gynecologic Malignancies 2Caris Life Sciences' Multiplatform Tumor Profiling Technology Identifies Expression of PD-1 and PD-L1 in Gynecologic Malignancies 3Caris Life Sciences' Multiplatform Tumor Profiling Technology Identifies Expression of PD-1 and PD-L1 in Gynecologic Malignancies 4Whitehouse Laboratories Reaffirms Commitment to Speedy Results – Renews Sponsorship of Berkenstock Racing Team 2
... 2011 Waste Management announced today ... facility at Eagle Valley Recycling and Disposal Facility in Orion, ... is helping power more homes across southeast Michigan through an ... this facility ensures the waste we generate will be converted ...
... Andrew R. Barron, a scientific co-founder of Natcore Technology ... for a prestigious World Technology Award presented by the ... CNN, Science/AAAS, and Technology Review.   Barron joins a ... around the world deemed to be doing the most ...
... A team of researchers from Roche ( ... Pharma Research and Early Development and Roche NimbleGen, reported inGenome ... sequence of the cynomolgus monkey ( Macaca fascicularis ) and ... for use in preclinical drug safety research. The ...
Cached Biology Technology:Waste Management Begins Production at its Eagle Valley Green Energy Plant 2Natcore Technology's Barron Named Finalist for World Technology Award 2Roche Researchers Sequence Complete Genome of Cynomolgus Monkey and Develop Novel Gene Expression Microarray for Drug Safety Assessment 2Roche Researchers Sequence Complete Genome of Cynomolgus Monkey and Develop Novel Gene Expression Microarray for Drug Safety Assessment 3Roche Researchers Sequence Complete Genome of Cynomolgus Monkey and Develop Novel Gene Expression Microarray for Drug Safety Assessment 4
(Date:3/17/2015)... March 17, 2015 Emotient, the leader ... today announced general availability of Emotient Analytics ... the analysis of facial expressions. The system analyzes ... content, products and services. It delivers audience response ... sentiment - as derived from facial evidence of ...
(Date:3/16/2015)... , March 16, 2015 ... GmbH will present groundbreaking innovations in biometric identification ... Germany .      (Photo: ... biometric innovation is well at the forefront: the ... e-gate made in Germany . ...
(Date:3/12/2015)... 12, 2015 The mascots of the Atlantic ... part of Wake Forest Baptist Medical Center. They stopped ... 2015 ACC Men,s Basketball Tournament. Young patients enjoyed bonding ... getting their autographs. Brenner Children,s Hospital is ... the only children,s hospital in northwest North ...
Breaking Biology News(10 mins):New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 2New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 3New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 4DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 2DERMALOG Presents its Latest Biometric Innovations at the Hannover CeBIT Exhibition, 16 - 20 March 2015 3
... 20, 2010) By emulating nature,s design principles, a ... Harvard Medical School and Dana-Farber Cancer Institute has created ... programmed to move and change shape on demand. In ... suitable for medical applications because DNA is both biocompatible ...
... National Institutes of Health, an agency of the U.S. ... Trust, a global charity based in London, today announced ... of common, non-communicable disorders such as heart disease and ... The studies, to be conducted by African researchers, ...
... most comprehensive comparative analysis to date of bacterial communities ... new insights into why some individuals become colonized with ... findings today in mBio the online open-access journal published ... and throat are important sites of pathogen colonization, yet ...
Cached Biology News:Researchers create self-assembling nanodevices that move and change shape on demand 2NIH and Wellcome Trust announce partnership to support population-based genome studies in Africa 2NIH and Wellcome Trust announce partnership to support population-based genome studies in Africa 3Study examines, compares bacteria in the nose and throat 2
ABgene® supplies a 94kDa ultra-pure recombinant thermostable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli....
... numbers of samples may be carried out using ... minimum operator attention. System control and data ... CE 4900. The elegant, powerful and very ... and Windows 98 and 2000., The supplied automatic ...
... 4100 high pressure dual piston pump and the ... and utra low drift. The WaveQuest ... detection and provides superior stability. Hence ... and for monitoring to the highest of sensitivities. ...
Mouse monoclonal [YPF19] to Yersinia pestis F1 antigen ( Abpromise for all tested applications)....
Biology Products: