Monitoring fluorescence resonance energy transfer (FRET) between GFP,,, fusions in lysates of the yeast Saccharomyces cerevisiae using the ,,, Varian,,, Cary Eclipse
Monitoring fluorescence resonance energy transfer (FRET) between GFP
fusions in lysates of the yeast Saccharomyces cerevisiae using the
Paul Gavin# and Mark Prescott#, Ph.D
Daren J. Fyfe, Ph.D*
# Department of Biochemistry and Molecular Biology, Monash University,
Clayton campus Victoria 3800, Australia
* Technical assistance: Varian Australia Pty Ltd, Mulgrave, Victoria 3170,
Australia E-mail: firstname.lastname@example.org
Fluorescence resonance energy transfer (FRET) is a non-destructive, spectroscopic
approach that can be used to monitor the proximity and angular orientation of
donor and acceptor fluorophores in living cells1. The resonant energy of an
excited donor fluorophore (in this example blue fluorescent proteinBFP) is
absorbed by an acceptor fluorophore (green fluorescent proteinGFP) providing
that donor and acceptor are in close proximity (between 1080 ngstroms2) (Figure
1). Emission spectra of donor fluorophores must significantly overlap the absorption
spectra of the acceptor, while overlap between respective absorption and emission
spectra of donor and acceptor should be minimized 3 .
Due to the attractive attributes of GFP (previously described in fluorescence
application note No. 5)4, the application of FRET to GFP and GFP variants has
become a powerful tool to monitor interactions at the protein level, within
an intact cell or organism. It is possible to use FRET to measure conformational
changes in a molecule tagged with two GFP variants in response to the binding
of ligands such as calcium1, or the interaction betwPage: All 1 2 3 4 Related biology technology :1
. Versatile Reporter Vectors for Monitoring Viral Transduction2
. Monitoring transfection efficiency by
green fluorescent protein (GFP) detection
with the Agilent 2100 bioanalyzer3
. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System4
. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis5
. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS6
. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation7
. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays8
. Immunoassay of Theophylline by fluorescence polarisation9
. Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP10
. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives
in the yeast Saccharomyces cerevisiae: Detection in vivo using
the Varian Cary Eclipse11
. Saccharomyces cerevisiae
(Date:8/27/2015)... Aug. 27, 2015 The National Necrotizing ... eyes—and life—were saved by doctors at Vanderbilt University Hospital ... NNFF, NovaBay Pharmaceuticals and Dr. John Crew , ... at Seton Medical Center in Daly City, ... a breakthrough approach, pioneered by Dr. Crew, that had ...
(Date:8/27/2015)... Inc. (TSXV: BRM) ("Biorem" or "the Company") today announced its results ... complete second quarter financial statements and MD&A have been filed on ... per shareThree-months ended , Six -months endedJune 30 , June 30 ... 2014 Revenue , 4,709 , 2,459 ... 1,656 , 56 , 3,188 , ...
(Date:8/27/2015)... YORK , August 27, 2015 ... enhancing image processing functions in the human vision system, announced ... point guard Chris Paul to develop ... players who want to improve their real-life on-court performance. ... the court, whether it is shooting a ball or blocking ...
(Date:8/27/2015)... ... 2015 , ... METTLER TOLEDO's web-based EasyFinder™ tool ... to a manageable shortlist of bench and floor scales and terminals. Specifying a ... with the customer’s industry and application. , Side-by-side product comparisons and an ... Breaking Biology Technology:Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3BIOREM Reports $718,000 Net Earnings for the Second Quarter 2BIOREM Reports $718,000 Net Earnings for the Second Quarter 3Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 2Eight-Time All-Star Chris Paul and GlassesOff Partner to Develop Innovative Mobile App Designed to Improve On-Court Performance 3METTLER TOLEDO Updates Online Scale Selection Tool 2
... ... about fast, new treatment program for FIV and FELV , ... New York, N.Y. (PRWEB) November 4, 2009 -- IMULAN BioTherapeutics, LLC ... fast way to get LTCI for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). ...
... ... To Target Tissues Affected by Osteoarthritis. Physics and Biology are Natural Partners in the ... ... medical device, the MyPhield Arthritis Therapy Cushion that naturally relieves osteoarthritis without causing unwanted ...
... Nov. 3 VIVUS, Inc. (Nasdaq: VVUS ... of novel therapeutic products, today reported its highlights and financial ... , Third Quarter Highlights , In ... two final, phase 3 pivotal 56-week studies, EQUIP (OB-302) and ...Cached Biology Technology:New Program Developed in the Fight Against Feline Aids 2New Technology Now Available In Canada For Osteoarthritis 2VIVUS Reports Third Quarter 2009 Highlights and Financial Results 2VIVUS Reports Third Quarter 2009 Highlights and Financial Results 3VIVUS Reports Third Quarter 2009 Highlights and Financial Results 4VIVUS Reports Third Quarter 2009 Highlights and Financial Results 5VIVUS Reports Third Quarter 2009 Highlights and Financial Results 6VIVUS Reports Third Quarter 2009 Highlights and Financial Results 7VIVUS Reports Third Quarter 2009 Highlights and Financial Results 8
(Date:8/19/2015)... , Aug. 19, 2015 VOXX International Corporation ... has entered into a Definitive Agreement to purchase ... iris authentication market leader EyeLock through an acquiring ... have a controlling interest in the acquiring entity. ... completion of due diligence. Expanding on its existing ...
(Date:8/12/2015)... 12, 2015 MedNet Solutions , an innovative ... of clinical research, is proud to announce that ... SaaS-based eClinical technology platform, has led the way to ... quarters of 2015. Q2 2015 and Q1 2015 ... by contract value sold in the company,s 15 year ...
(Date:8/11/2015)... Today, ZUK announced its Android smartphone Z1, featuring FPC,s touch ... 2015 that relate to sales of FPC1155 for this smartphone model ... for 2015. Jörgen Lantto, CEO of FPC, comments: " ... manufacturer in China and we are proud ... for Z1 , its first ... Breaking Biology News(10 mins):VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 2VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 3VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 4VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 5MedNet Solutions Experiences Explosive Corporate Growth 2FPC's Touch Fingerprint Sensor FPC1155 in ZUK's Smartphone Z1 2
... This product is expected to react ... species but has not been specifically ... precipitin line against partially purified rabbit ... react with all forms of Pyruvate ...
Rabbit polyclonal to Somatostatin 28 ( Abpromise for all tested applications). entrezGeneID: 6750 SwissProtID: P61278...
... Convenient platform for tube handling and reagent ... 0.2ml V-bottom tubes - 96 x ... - Thermo-Fast® 96 Non-Skirted Plate ... - 48 x 0.5ml Thermo-Tubes Multiple workstations can be ...
... Pro upgrade for Shimadzu Phoretix 2D, Expression ... statistical tools for comparative gel analysis studies ... Data base add on component ... version 9i release 2 client software). ...Biology Products: