Navigation Links
Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data

escent RT-IVT Labeling Kit.

The labeled DIG-cRNA (10 μg per microarray) was injected into each microarray hybridization chamber. Following hybridization at 55C for 16 hours, the unbound material was washed from the microarrays. Features that retained bound DIG-labeled cRNA were visualized using the Applied Biosystems Chemiluminescence Detection Kit. The kit uses anti-DIG alkaline phoshatase that hydrolyses a chemiluminescent substrate to generate light at 458 nm which can be analyzed using the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer.

Results

Technical and Biological Replicates The reproducibility of the Applied Biosystems Expression Array System was demonstrated by comparison of technical replicates. Global median normalization was performed for each microarray and subsequent values were then log transformed. To assess variation in gene expression, background noise was filtered out for each pair of arrays by using only datawith signal-to-noise ratios greater than 3 (Figure 2A, Table 1). Additionally, we compared biological replicates (Biological_rep1 vs. Biological_rep2) between two individuals with the same disease condition (Figure 2B).

Fold Changes of Differential Gene Expression The fold change of normal vs. metastasis tumor sample was analyzed by filtering the dataset using p-values < 0.01 and a signal-to-noise ratio > 3 for use in ANOVA statistical analysis. Of these 2,508 genes, 623 genes were up-regulated and 435 genes were down-regulated. The number of detected genes for the corresponding p-values of < 0.05 and < 0.001 were 5,703 and 796 genes, respectively (Table 2). Using the Spotfire software application, fold changes can be determined for the differentially expressed genes (Figure 3).

Clustering Analysis

From the ANOVA statistical analysis (p < 0.01) the identified 2,508 genes were sele
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes
2. Protocol for GenomePlex Whole Genome Amplification from Buccal Swab
3. Protocol for GenomePlex Whole Genome Amplification from Blood Card
4. Protocol for GenomePlex Whole Genome Amplification from Plant
5. Protocol for GenomePlex Whole Genome Amplification from Soil
6. Protocol for GenomePlex Whole Genome Amplification from Whole Blood
7. Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue
8. Bacterial Whole Genome Array Analysis
9. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
10. Oragene and Whole Genome Amplification with GenomiPhi
11. Amplification of genome-representative DNA from limited sources with GenomePlex WGA technology for use in genetic alterations studies
Post Your Comments:
(Date:8/19/2014)... August 19, 2014 Robin Williams’ passing ... can take on an individual. Symptoms range from ... expression, problems swallowing and severe depression. Parkinson’s disease ... off of dopamine producing neurons of the brain. ... disease that slowly and progressively gets worse, they look ...
(Date:8/19/2014)... 2014 Research and Markets ... "Spectroscopy Equipment and Accessories - Global ... offering. This report analyzes ... Accessories in US$ Thousands by the following ... Spectrometers, NMR-EPR Spectrometers, Near Infrared (NIR) Spectrometers, ...
(Date:8/19/2014)... 19, 2014 In November 2012, the European ... Evaluation Report (PBRER) in a new PSUR – responding to ... benefit-risk profile of a drug. Adopted in many regions, the ... Present a comprehensive and critical analysis of new or ... Highlight any new evidence of the potential benefits of ...
(Date:8/19/2014)... - iCo Therapeutics Inc. ("iCo" or "the Company") (TSX-V: ... Oral Amphotericin B (Oral Amp B) drug candidate ... ImmuneCarta®, the immune monitoring business unit of Caprion, ... Amp B in reactivating latent HIV viral reservoirs ... with antiretroviral therapy. Memory cells, ...
Breaking Biology Technology:As We Mourn Robin Williams’ Passing, His Death Sheds Light on Patients Struggling with Parkinson’s Disease 2As We Mourn Robin Williams’ Passing, His Death Sheds Light on Patients Struggling with Parkinson’s Disease 3Global Spectroscopy Equipment and Accessories - Strategic Business Report 2014 2Top Five Best Practices for Developing Your Periodic Benefit Risk Evaluation Report (PBRER), New Webinar Hosted by Xtalks 2iCo Therapeutics Announces Positive Oral Amphotericin B Study Results 2iCo Therapeutics Announces Positive Oral Amphotericin B Study Results 3
... ability to drive clients, success in managed care, ... Group, a,full-service managed market agency focused on securing ... of Dan Renick, RPh,as Managing Partner. Renick will ... biotech clients, including GlaxoSmithKline,sanofi-aventis, and Forest Laboratories., ...
... Abbott will present,at the Goldman Sachs Twenty-Ninth ... Thomas C. Freyman, executive vice president,finance and chief ... at 3:20 p.m. Central time., A live ... through,Abbott,s Investor Relations Web site at http://www.abbottinvestor.com ...
... China, May 28 /Xinhua-PRNewswire-FirstCall/ -- 3SBio Inc.,(Nasdaq: ... focused on researching,developing, manufacturing and marketing biopharmaceutical ... Chief Operating Officer, and,Kevin Teo, Chief Financial ... Annual Global Healthcare Conference in Dana Point, ...
Cached Biology Technology:Dan Renick, RPh, Joins The Hobart Group as Managing Partner 2
(Date:8/20/2014)... In leaves, photosystem 1 (PS1) absorbs light and ... of carbon dioxide to biomass. Photovoltaic devices, mostly ... light but produce electricity. One approach for the ... replacing the semi-conductor with the isolated membrane protein ... isolates a highly stable PS1 from thermophilic cyanobacteria ...
(Date:8/19/2014)... China,s increasing caesarean section rate, suggests a new commentary ... Journal of Obstetrics and Gynaecology (BJOG) . , China ... the world. Of 16 million babies born in 2010, ... is not known, the current Chinese language literature on ... from 36% to 58%. However, before the 1980s, the ...
(Date:8/19/2014)... Wind and ... amount of electricity is to be produced with renewable energy ... stored during productive periods so that these fluctuations can be ... for the purpose. Science and industry are therefore working on ... that used to be too expensive or unsophisticated to be ...
Breaking Biology News(10 mins):A semi-artificial leaf faster than 'natural' photosynthesis 2Is China's 50 percent cesarean section delivery rate too high? 2Is China's 50 percent cesarean section delivery rate too high? 3Asian inventions dominate energy storage systems 2Asian inventions dominate energy storage systems 3
... (March 28, 2011)Understanding that angioplastya medical treatment used by ... and chest to improve blood flowis safe may encourage ... for individuals with multiple sclerosis, say researchers at the ... Chicago, Ill. "Angioplastythe nonsurgical procedure of threading a ...
... March 28, 2011 University of Utah scientists used ... and toadfish inner-ear cells send signals to the brain. ... and lead to devices to restore vision, maintain balance ... to talk to the brain with optical infrared pulses ...
... JOLLA, CA Over the last years, two teams of ... model of how a powerful DNA repair complex works. Now, ... molecular motor inside the complex functions findings they say ... to cystic fibrosis. In a paper published in an ...
Cached Biology News:Interventional radiologists advance MS research: Vein-opening treatment safe 2Interventional radiologists advance MS research: Vein-opening treatment safe 3Interventional radiologists advance MS research: Vein-opening treatment safe 4Will we hear the light? 2Will we hear the light? 3Will we hear the light? 4Will we hear the light? 5Structure of DNA repair complex reveals workings of powerful cell motor 2Structure of DNA repair complex reveals workings of powerful cell motor 3Structure of DNA repair complex reveals workings of powerful cell motor 4
... These handy dispensers save space and ... and other garb. They are the ... parts used in labs, processing and ... charges and the particles they attract. ...
... These handy dispensers save space and ... and other garb. They are the ... parts used in labs, processing and ... charges and the particles they attract. ...
... that it uses a deep-UV deuterium bulb, which ... with a shutter (controlled via a TTL signal ... Source produces a powerful, stable output from 215-400 ... source are available, providing a wavelength range of ...
... and mitochondrial membrane potential. The mitochondrial permeability ... the initiation of cellular apoptosis. This process ... the electrochemical gradient across the mitochondrial membrane, ... membrane potential (YD). Loss of mitochondrial YD ...
Biology Products: