In the application note, researchers at the New Zealand Institute for Plant & Food Research describe their experience extracting DNA for species determination from samples of three distinct insect species typically found on kiwifruit, a major New Zealand food crop. They performed extractions with ZyGEM's prepGEM® Insect kit, then ran the samples through a multiplex PCR. The prepGEM® Insect kit extracted sufficient DNA to serve as a template for PCR with a single temperature-controlled reaction using its unique combination of thermophilic enzymes and a buffer with proprietary agents that enhance cell lysis. Results showed that prepGEM® Insect's simple reaction in a single closed tube efficiently extracted high quality DNA from all of the insect sample types.
The prepGEM® Insect kit is compatible with all PCR detection and amplification reagents. The stability of the prepGEM® reagents allows convenient storage of the kit at 4°C, reducing preparation time and increasing ease of use.
ZyGEM offers a growing number of application notes for its prepGEM®, forensicGEM® and livestockGEM® families of kits. ZyGEM application notes can be accessed at www.zygem.com/Products/Products-Pubs.html.
ZyGEM's proprietary technology uses a thermophilic enzyme to extract DNA from samples using a single closed-tube system. ZyGEM's approach significantly reduces the time and cost of DNA extractions while simplifying laboratory workflow, minimizing error, reducing the risk of contamination, ensuring sample integrity and facilitating extractions from trace samples. It is easily adaptable for use with virtually all off-the-shelf laboratory automation systems. The technology can be spe
|SOURCE ZyGEM Corp. Ltd.|
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