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World's smallest reaction chamber
Date:12/6/2012

Scientists from New Zealand, Austria and the UK have created the world's smallest reaction chamber, with a mixing volume that can be measured in femtolitres (million billionths of a litre).

Using this minuscule reaction chamber, lead researcher Peter Derrick, professor of chemical physics and physical chemistry and head of the Institute of Fundamental Sciences at Massey University in New Zealand, plans to study the kind of speedy, nanoscale biochemical reactions that take place inside individual cells. This work appears in the latest issue of the European Journal of Mass Spectrometry.

The reaction chamber actually consists of nothing more than a tiny spray of liquid. It is produced by a technique known as electrospray ionization, in which a liquid is converted into lots of charged droplets by exposing it to a high voltage as it exits the nozzle of a thin capillary.

Like water being sprayed out of a hose, these charged droplets form a cone shape, known as a Taylor cone, as they are emitted from the nozzle. Because the electrospray process transforms any chemical entities within the liquid into ions, it is a commonly used technique for ionizing a liquid sample prior to analysis by mass spectrometry.

In conventional electrospray ionization, the charged droplets are emitted from a single nozzle and form a single Taylor cone. Derrick realised that emitting charged droplets with different chemical compositions from two separate but adjoining nozzles would cause their respective Taylor cones to merge, potentially allowing the chemical entities in those droplets to react together.

By making the nozzles small enough, such that they produce Taylor cones with femtolitre volumes, and linking them to a mass spectrometer, this set-up could be used to study the kind of speedy, nanoscale biochemical reactions that take place within cells. "The idea was that the entities could be introduced separately through the two chann
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Contact: Professor Peter Derrick
p.j.derrick@massey.ac.nz
IM Publications LLP
Source:Eurekalert  

Page: 1 2

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