Navigation Links
Venter Institute Scientists Create First Synthetic Bacterial Genome
Date:1/24/2008

create 25 subassemblies, each about 24,000 base pairs (24kb). These 24kb fragments were cloned into the bacterium Escherichia coli to produce sufficient DNA for the next steps, and for DNA sequence validation.

The next step involved combining three 24kb fragments together to create 8 assembled blocks, each about 72,000 base pairs. These 1/8th fragments of the whole genome were again cloned into E. coli for DNA production and DNA sequencing. Step three involved combining two 1/8th fragments together to produce large fragments approximately 144,000 base pairs or 1/4th of the whole genome.

At this stage the team could not obtain half genome clones in E. coli, so the team experimented with yeast and found that it tolerated the large foreign DNA molecules well, and that they were able to assemble the fragments together by homologous recombination. This process was used to assemble the last cassettes, from 1/4 genome fragments to the final genome of more than 580,000 base pairs. The final chromosome was again sequenced in order to validate the complete accurate chemical structure.

The synthetic M. genitalium has a molecular weight of 360,110 kilodaltons (kDa). Printed in 10 point font, the letters of the M. genitalium JCVI-1.0 genome span 147 pages.

"This is an exciting advance for our team and the field. However, we continue to work toward the ultimate goal of inserting the synthetic chromosome into a cell and booting it up to create the first synthetic organism," said Dan Gibson, lead author.

The research to create the synthetic M. genitalium JCVI-1.0 was funded by Synthetic Genomics, Inc.

Background/Key Milestones in JCVI's Synthetic Genomics Research

The work described by Gibson et al. has its genesis in research by Dr. Venter and colleagues in the mid-1990s after sequencing M. genitalium and beginning work on the minimal genome project. This area of research, trying to understand the minimal genetic compone
'/>"/>

SOURCE J. Craig Venter Institute
Copyright©2008 PR Newswire.
All rights reserved

Page: 1 2 3 4 5 6

Related biology technology :

1. AstraZeneca and Banner Alzheimers Institute Collaborate to Evaluate New Tool Targeting Alzheimers Disease
2. National Cancer Institute Study to Determine the Optimal Time for CLL Patients to Begin Treatment
3. Digital Healthcare Announces Cincinnati Eye Institute is Latest Retasure Reading Center
4. Discovery Institute Bioethicist Lauds Breakthrough in Stem Cells Research that Eliminates Need for Human Cloning
5. The USC Stevens Institute for Innovation Opens Shop on USC Health Sciences Campus
6. Infectious Disease Research Institute Receives Grant for Acquisition of Core Research Equipment from M.J. Murdock Charitable Trust
7. The USC Stevens Institute for Innovation Opens Shop on US Health Sciences Campus
8. National Institutes of Health Awards $25.5M Contract to SRA
9. Caliper Life Sciences Awarded National Institute of Environmental Health Services Contract Valued to $7.0 Million
10. HUYA Bioscience International Licenses Clinical Stage Anti-Arrhythmic Compound from the Shanghai Institute of Materia Medica
11. Solazyme Selected for National Institute of Standards and Technology Award
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/22/2014)... 2014 “Our nation's healthcare system ... national disgrace. Furthermore, every governmental and professional entity ... deplorable state of unorganized dysfunction shares in that ... merely serve as a device to use in ... failure(s) that have accumulated through the years leading ...
(Date:8/21/2014)... A Finnish-Swedish research group at the Institute for ... Karolinska institutet, Stockholm, has developed a novel "man ... infection. This innovative diagnostic aid was described in ... August. The method is based on computer vision ... systems combined with visualization of only the diagnostically ...
(Date:8/21/2014)... August 21, 2014 ... has announced the addition of the  "Global ... their offering.       (Logo: http://photos.prnewswire.com/prnh/20130307/600769) ... therapy, is a means of replacing hormones ... the body. This type of therapy is ...
(Date:8/21/2014)... N.Y. (PRWEB) August 21, 2014 The ... the first private college in the Mid-Hudson Region to ... will now begin accepting applications from qualified “high-technology” businesses ... “We are very pleased to have been selected for ... Governor Cuomo’s transformative initiative to stimulate economic development in ...
Breaking Biology Technology:5 Great Mistakes in Healthcare and How to Fix Them by Dr. Ira Williams 2A novel 'man and machine' decision support system makes malaria diagnostics more effective 2Global Hormone Replacement Therapy Market 2014-2018: Key Vendors are Actavis, Bayer, Merck & Co, Novartis and Novo Nordisk 2The College of New Rochelle Becomes First Private College in Mid-Hudson Region to Earn START-UP NY Designation 2The College of New Rochelle Becomes First Private College in Mid-Hudson Region to Earn START-UP NY Designation 3
... - Additional AeroLEF patents granted in Europe , ... , Feb. 10 /PRNewswire-FirstCall/ - YM BioSciences Inc. ... two additional patents in the US for AeroLEF(R), the Company,s ... for the treatment of moderate to severe acute pain. US ...
... ... Separation Training on March 22nd in San Antonio, Texas, USA. AFS is holding its 23rd ... There will be eleven courses held on March 22nd. Six of these courses are on ... ...
... you don,t swallow the worm at the bottom of a ... worminess of the potent Mexican liquor, according to scientists from ... Guelph. , They have discovered that the liquid itself ... famously tasty mescal "worm". , As part of a study ...
Cached Biology Technology:YM BioSciences granted two US patents for AeroLEF(R) 2YM BioSciences granted two US patents for AeroLEF(R) 3YM BioSciences granted two US patents for AeroLEF(R) 4Water Filtration and Liquid Separation Training by the American Filtration and Separations Society. 2Water Filtration and Liquid Separation Training by the American Filtration and Separations Society. 3Water Filtration and Liquid Separation Training by the American Filtration and Separations Society. 4Water Filtration and Liquid Separation Training by the American Filtration and Separations Society. 5Mescal worm test shows DNA leaks into preservative liquids 2
(Date:8/21/2014)... in smog-producing toxins in past decade, GTA still ... shows that while the Greater Toronto Area (GTA) ... contribute to smog, the city continues to violate ... which can cause or aggravate health problems such ... by a set of complex photochemical reactions involving ...
(Date:8/21/2014)... -- Nxt-ID, Inc. (OTCQB: NXTD), a biometric authentication company ... that its shares of common stock and the warrants ... proposed underwritten public offering of common stock and warrants ... Market, subject to closing of its proposed underwritten public ... trade under the symbol "NXTD" and "NXTDW," respectively. ...
(Date:8/21/2014)... to ignore, but they are the ultimate source of ... are increasingly dependent on algae, too, to suck up ... to the bottom of the ocean. Now, by using ... have evidence showing that viruses infecting those algae are ... when all else stays essentially the same, and this ...
Breaking Biology News(10 mins):Despite a significant reduction in smog-producing toxins, the Greater Toronto Area still violates Canada's standards for ozone air pollution 2Nxt-ID Approved for NASDAQ Capital Market Listing 2Nxt-ID Approved for NASDAQ Capital Market Listing 3Viruses take down massive algal blooms, with big implications for climate 2
... bacteria destroy oil. Might those bacteria lead oil companies to ... while at the same time reducing the carbon dioxide that ... thinks that may be possible. Larter, professor of ... Geology at the University of Calgary, was the keynote speaker ...
... , MADISON Establishing a firm link between environmental ... proposition. Now, however, a team of scientists from ... 16, 2010) online issue of the CDC journal ... to date linking increased incidence of malaria to land-use ...
... found antibiotic-resistant bacteria in seven species of sharks and ... Massachusetts. Most of these wild, free-swimming fish harbored several ... the Journal of Zoo and Wildlife Medicine , ... The researchers also found multidrug-resistant bacteria in fish at ...
Cached Biology News:Incidence of malaria jumps when Amazon forests are cut 2Incidence of malaria jumps when Amazon forests are cut 3Wild sharks, redfish harbor antibiotic-resistant bacteria 2
This antibody detects ubiquitinated proteins on SDS-PAGE immunoblots. It does not detect free ubiquitin....
Lysozyme C (W-20)...
Request Info...
Abgene's ALTO-8 is the first automated decapper/capper for Abgene Twist-Lock tubes....
Biology Products: