Navigation Links
Venter Institute-Led Team Recovers and Sequences Genome of Periodontal Pathogen from Biofilm in Hospital Sink using Single-Cell Genomics
Date:4/5/2013

LA JOLLA, Calif., April 5, 2013 /PRNewswire-USNewswire/ -- A team of researchers led by scientists from the J. Craig Venter Institute (JCVI) has published a study outlining the recovery and genomic analysis, using single-cell genomic techniques, of a periodontal pathogen, Porphyromonas gingivalis, from a hospital sink. This is the first time that a single-cell genome sequencing approach was used to isolate and analyze a single microbe from a biofilm in a healthcare setting. The team, led by JCVI's Jeffrey McLean published their study in the April 5 edition of the journal Genome Research.

Understanding the community of microbes living in biofilms, especially those in healthcare settings, has been limited partially because pathogens can be in very low numbers and many other bacterial types are not easily cultured.  A method for DNA sequencing from single cells developed by JCVI's Roger Lasken group, is now allowing researchers to sequence the vast numbers of uncultured microbes in the environment. With this approach this team hopes to sequence many hospital pathogens that have been otherwise inaccessible. 

In this study the team targeted bacterial cells in a biofilm sampled from a hospital bathroom sink. Using single-cell genomic sequencing combined with a new single-cell genome assembler, SPAdes, developed by Pavel Pevzner , University of California, San Diego, the team found 25 different types of bacteria within the biofilm. The bacteria represented environmental species, human commensals and human pathogens.

The team then reconstructed a near complete genome of one specific periodontal pathogen, P. gingivalis (designated as JCVI SC001) from a single cell. While this globally important pathogen is well known, only three other P. gingivalis genomes have been sequenced to date, and a
'/>"/>

SOURCE J. Craig Venter Institute
Copyright©2012 PR Newswire.
All rights reserved

Page: 1 2 3

Related biology technology :

1. Human Genome Decoder and Artificial Life Creator, J. Craig Venter to Speak at "The Atlantic Meets the Pacific"
2. Richard H. Scheuermann, Ph.D. Joins J. Craig Venter Institute as Director of Informatics
3. Neural implant recovers ability to make decisions
4. Scientists Warn of Long Term Consequences of Budget Sequestration: BioInformatics LLC Report
5. Discovered Evolution of Genomic Sequences -- from Ocean Archaea to Brain Cancer -- Leads to New Synthetic Replikin Vaccines for Infectious Diseases and Cancer
6. Personal Genome Diagnostics Inc. Licenses Genome-Mapping Technology from Johns Hopkins University and Expands Its Cancer Genome Analysis Business
7. New York Genome Institute Selects Exemplar LIMS to Manage Next Gen Sequencing Labs
8. The Genome Analysis Center Selects Convey to Support Next-Generation Sequencing
9. Whole Genome Sequencing of Triple Negative Breast Cancer Reveals Previously Unreported Mutations
10. Pig genome offers insights into the feistiest of farm animals
11. Researchers unlock disease information hidden in genomes control circuitry
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/30/2014)... July 30, 2014   Epic Sciences, Inc. ... novel diagnostics to personalize and advance the treatment and ... Gregory T. Lucier as chairman of Epic,s board ... of Life Technologies (formerly Invitrogen). Mr. Lucier joins Epic,s ... its circulating rare cell analysis platform with special focus ...
(Date:7/30/2014)... July 30, 2014 NCERC at ... will present his findings on corn stover pretreatment ... this week in Washington, D.C. , “Arun’s ... conference is a testament to the success of ... said. “Thanks to the foresight and vision of ...
(Date:7/30/2014)... 30, 2014 Adding to their already ... boat special – Buy 2 of any Weighing ... will benefit lab technicians looking for durable and reliable ... ideal for multiple applications including weighing liquids, solid, or ... choices including diamond-shaped, hexagonal, square, aluminum weighing, and standard ...
(Date:7/29/2014)... 2014 SoundConnect , an ... is proud to announce the strategic additions of ... their Boston, Charleston and Atlanta offices. SoundConnect’s ... demand for hosted solutions, collaboration and mobility that ... cost. , SoundConnect was founded in 2004, with ...
Breaking Biology Technology:Epic Sciences Appoints Greg Lucier as Chairman of the Board of Directors 2Epic Sciences Appoints Greg Lucier as Chairman of the Board of Directors 3NCERC at SIUE Researcher Takes Stage at Department of Energy Conference 2NCERC at SIUE Researcher Takes Stage at Department of Energy Conference 3Weighing Boat Promotion Now Available at Pipette.com, Major Pipette Distributor 2SoundConnect Organization and Culture is Evolving 2
... plugs, cut the cords, and can the cables as we ... for all the freedom and mobility, we are nowhere without ... the like. That fact alone has turned the average business ... most obscure corner of airports and coffee houses. Indeed, Im ...
... Motorists driving along the DeNeveu Creek bridge on Highway 151 ... they drive over a bridge that uses a new method ... concern themselves with the cost of road construction may definitely ... road. , ,Instead of having a roadbed reinforced with a ...
... When it comes to consumer goods, America is addicted to ... to low prices, Wal-Mart is the biggest low-price outlet going. ... to get it. , ,The company that many Americans hate ... and activist groups have successfully derailed its growth plans in ...
Cached Biology Technology:Power for the people 2UW-Madison engineers apply award-winning technology to road building 2Wal-Mart: How low can you go? 2Wal-Mart: How low can you go? 3Wal-Mart: How low can you go? 4
(Date:7/30/2014)... pledge of $1 million from the Cornelia Cogswell Rossi ... Biometry Center. , The newly established Connie ... to develop a greater breadth and depth of knowledge ... the pace of research to improve human health. , ... for the Laboratory and its external partners, supporting research ...
(Date:7/30/2014)... ranges and a changing climate threaten some of ... and so conservationists establish seed collections to save ... hopes of preserving some genetic diversity., For decades, ... models that offer a one-size-fits-all approach for how ... 50 seed samples regardless of species, pollination mode, ...
(Date:7/30/2014)... from Bjorn Brembs, professor of neurogenetics at the Institute ... figure allowing readers and reviewers to run the underlying ... important leap forward for scientific publishing, by demonstrating a ... scholarly output. , Figure 3 in fact doesn,t really ... to F1000Research , and the figure is generated ...
Breaking Biology News(10 mins):Rossi Foundation pledges $1M for JAX neurobehavioral research center 2Saving seeds the right way can save the world's plants 2F1000Research brings static research figures to life 2
... have so far won 19 grants worth a total of ... Recovery and Reinvestment Act. The grants range from $2 ... produce biological hydrocarbons for biofuels to $600,000 for a study ... the Ebola virus can inhibit a body,s antiviral response. Fifteen ...
... gene defect in mice has helped identify a new protein ... study by University of Iowa and Kansas State University researchers. ... open-access journal PLoS Genetics . In humans, ... yet most genes involved in hearing are unidentified. Mice are ...
... book demystifies bats and eliminates many myths surrounding rabies ... Lyssavirus Infections , combines current science about bat rabies ... The author, Denny G. Constantine, is widely considered one ... Rabies is intended for scientists and the general public. ...
Cached Biology News:Iowa State faculty attract $4.8 million in American Recovery and Reinvestment Act grants 2Iowa State faculty attract $4.8 million in American Recovery and Reinvestment Act grants 3Iowa State faculty attract $4.8 million in American Recovery and Reinvestment Act grants 4Forget the garlic necklace -- learn more about bats and rabies 2
See product name for description....
Whole blood is collected from healthy pigs (mixed breed, either sex) at the time of slaughter, refrigerated within 6 hours, processed within 24 hours and frozen at -20ºC....
... DNA-binding protein(DBP). Protein size: ... NP_001343 ELISA titre using peptide based ... for Western Blot: 1.0g/ml in ... HRP conjugated anti-Rabbit IgG should be diluted ...
...
Biology Products: