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Transgenomic Introduces SURVEYOR(R) Endonuclease Adaptor-Ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation
Date:6/17/2008

Officer and President of Transgenomic commented, "We expect SEAL to have a significant impact on the use of DNA sequencing within pharmacogenomic assessments for personalized medicine. A whole genome catalog of sequence variations below $10,000 is a realistic goal based on our prediction that SEAL should identify point mutations and small insertion/deletions at 100 to 1000 times lower sequencing intensity than current methods."

About SEAL

SURVEYOR Endonuclease Adaptor-ligated Libraries (SEAL) is a cost-effective and high throughput enabling technology for whole genome analysis under development by Transgenomic. It identifies DNA variations between a reference genome and a test genome. Using a linker that attaches an identification tag of known sequence to the ends cleaved by the SURVEYOR Nuclease, SEAL generates a library of genetic variations present within a DNA sample relative to a reference sample, allowing recognition of both the sequence of the variant nucleotide and the sequence context of the mutation. The library can be sequenced directly via standard or massively parallel systems, queried at any desired genetic locus by PCR amplification or archived for subsequent multi-query analysis.

About SURVEYOR

SURVEYOR Mutation Detection Kits and the SURVEYOR Check-It Kit provide reagents and protocols for the detection of mutations in DNA. All of these kits are based on the use of SURVEYOR Nuclease, a mismatch-specific endonuclease shown to recognize and cleave all types of mismatches arising from the presence of single nucleotide polymorphisms (SNPs) or from small insertions or deletions. The protocol is based on the generation of PCR products that are subsequently hybridized to generate mismatches in heteroduplexed DNA which is then treated and cleaved by SURVEYOR Nuclease. SURVEYOR Nuclease can detect genetic variations in samples with high sensitivity.

About Transgenomic

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