Navigation Links
Texas A&M chemical engineer's work could lead to improved DNA analysis
Date:9/9/2010

COLLEGE STATION, Sept. 9, 2010 DNA analysis is poised to experience a significant advancement thanks to the work of a Texas A&M University chemical engineer, who has discovered a way to achieve more effective separation of DNA fragments.

Working with a widely used gelatin substance known as a hydrogel, Victor M. Ugaz, associate professor in the university's Artie McFerrin Department of Chemical Engineering, and graduate student Nan Shi have been able to determine the specific type of conditions that result in the optimum gel pore structure for separation of a wide range of DNA fragment sizes. Their findings appear in the Sept. 3 edition of the journal Physical Review Letters.

"It changes the way you think about the entire process because these findings demonstrate a rational way to connect the pore structure of the gel quantitatively to the mechanism by which the DNA moves through the gel," Ugaz explains. "Researchers can now actually design gels to specifically harness certain effects, and they will need this information we have found to do that."

The enhanced separation technique, Ugaz notes, could benefit a wide array of fields that utilize DNA analysis, including biomedical research, forensics and genetic engineering.

Key to Ugaz's findings is the manner in which DNA fragments move through a hydrogel. Employing a process called "electrophoresis," researchers who study DNA typically embed negatively charged DNA into a porous hydrogel. They then apply an electric field which causes the DNA fragments to move through the pores of the hydrogel. Naturally, smaller DNA chains move faster through the maze of pores than longer strands of DNA.

However, when DNA chains are roughly the same size as the pores through which they are attempting to pass, a process called "entropic trapping" takes place, Ugaz notes. During this process, the naturally coiled DNA fragment, in a sense, has to unthread a bit to pass through a pore, he says. Because the fragment wants to return to its coiled shape, it quickly squeezes through the smaller pore so that it can enter a larger pore where there is enough room for it to return to its natural shape.

Harnessing this entropic trapping effect for separation through a hydrogel marks a significant advancement in DNA studies, Ugaz says.

Although it has long been predicted that entropic trapping effects can potentially benefit a wide variety of applications including separation technologies, actually figuring out how to use this phenomenon previously has been difficult in hydrogels because it has not been clear how this transport mechanism is linked to the gel's porous structure, Ugaz explains.

In other words, hydrogels need to have very specific properties such as pore size distribution, and prior to these findings, there has been no way to know how to choose the right hydrogel that has the right properties, Ugaz notes.

"You want to be able to detect the smallest possible difference in size between DNA fragments," Ugaz explains. "The size of the fragments may be very close, and you may need to detect a difference of one unit in size. To do this, you would want to be able to specifically construct a hydrogel with the necessary pore structure to achieve this."

Ugaz's research provided the "instructions on how to do just that.

"We have a better picture of how to do this than what has existed," Ugaz says. "We know what the gel needs to look like and how it needs to be prepared.

"We're able to understand how to construct a gel that would allow DNA to move via an entropic trapping method that enhances separation performance and in turn leads to more effective analysis. This finding could have enormous implications by helping remove current barriers to separation efficiency"


'/>"/>

Contact: Ryan Garcia
ryan.garcia99@tamu.edu
979-845-9237
Texas A&M University
Source:Eurekalert

Related biology technology :

1. LGE Execs Teams with Texas Tech to License Advancements in Agriculture, Health and Life Sciences
2. Annual List of Best Doctors in Dallas Showcases Texas Institute for Surgery Physicians
3. Study From the University of Texas M.D. Anderson Cancer Center Demonstrates That AHCC(R) is Safe in Combination With Most Chemotherapeutic Agents
4. SpineSmith Partners Collaborates with University of Texas Biomedical Engineering Students to Develop Innovative Products
5. Self-powered devices possible, says Texas A&M researcher
6. NSTI Announces the 12th Annual Nanotech Conference & Expo 2009 May 3-7, at the George R. Brown Convention Center in Houston, Texas
7. Panda Ethanols Hereford, Texas Subsidiary Files Voluntary Chapter 11
8. WaferGen Biosystems, ImmuneRegen BioSciences and University of Texas Southwestern Medical Center to Collaborate on Novel Wound Healing Research
9. Z Therapy Is The First In North Texas To Offer Revolutionary Treatment Option For Neck Pain Sufferers
10. Solar cells, geological storage research receive DOE funding at the University of Texas at Austin
11. Dozens of New Clean Technology and Nanotech Products Unveiled at TechConnect World Expo in Houston, Texas, May 5-6
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/9/2016)... ... February 09, 2016 , ... The publishing industry ... Access publishing is one of the popular publication models that has received wider ... and 3000+ International Conferences across the globe, OMICS International is ...
(Date:2/9/2016)...  DNAtrix, a clinical stage, biotechnology company ... its lead product, DNX-2401, has been designated ... medicinal product for the treatment of glioma. ... approximately 25,000 people a year in the ... --> http://photos.prnewswire.com/prnh/20160208/330986LOGO ...
(Date:2/9/2016)... BOSTON and LONDON , February ... see leading bio tech replace paper and ... The web-based electronic laboratory notebook (ELN) will be rolled out ... replace paper-based research and development (R&D) and protect valuable IP. ... time and follow a specific researcher or experiment as part ...
(Date:2/9/2016)... , Feb. 9, 2016 BERG, ... data-driven, biological research approach, has announced the appointment ... Officer and Chief Operating Officer. Haddock brings to ... finance, including 12 years in senior financial functions ... experience in business organizational management. ...
Breaking Biology Technology:
(Date:2/2/2016)... 2016   Parabon NanoLabs (Parabon) announced ... Research Office and the Defense Forensics and Biometrics ... the company,s Snapshot Kinship Inference software ... generally, defense-related DNA forensics.  Although Snapshot is best ... and ancestry from DNA evidence), it also has ...
(Date:2/1/2016)... wallet ( www.wocketwallet.com ) announces the launch of a new video featuring ... Las Vegas , where Joey appeared at the Wocket booth ... , where Joey appeared at the Wocket booth to meet and ... at the Consumer Electronics Show (CES2016) in Las Vegas ... fans. --> --> The video ...
(Date:1/25/2016)... 25, 2016  Glencoe Software, the world-leading supplier of ... industries, will provide the data management solution OMERO Plus ... Photo - ... Phenotypic analysis measures the characteristics and ... comparisons between states such as health and disease, the ...
Breaking Biology News(10 mins):