Navigation Links
Tenfold boost in ability to pinpoint proteins in cancer cells
Date:3/19/2013

Better diagnosis and treatment of cancer could hinge on the ability to better understand a single cell at its molecular level. New research offers a more comprehensive way of analyzing one cell's unique behavior, using an array of colors to show patterns that could indicate why a cell will or won't become cancerous.

A University of Washington team has developed a new method for color-coding cells that allows them to illuminate 100 biomarkers, a ten-time increase from the current research standard, to help analyze individual cells from cultures or tissue biopsies. The work is published this week (March 19) in Nature Communications.

"Discovering this process is an unprecedented breakthrough for the field," said corresponding author Xiaohu Gao, a UW associate professor of bioengineering. "This technology opens up exciting opportunities for single-cell analysis and clinical diagnosis."

The research builds on current methods that use a smaller array of colors to point out a cell's biomarkers characteristics that indicate a special, and potentially abnormal or diseased, cell. Ideally, scientists would be able to test for a large number of biomarkers, then rely on the patterns that emerge from those tests to understand a cell's properties.

The UW research team has created a cycle process that allows scientists to test for up to 100 biomarkers in a single cell. Before, researchers could only test for 10 at a time.

The analysis uses quantum dots, which are fluorescent balls of semiconductor material. Quantum dots are the smaller version of the material found in many electronics, including smartphones and radios. These quantum dots are between 2 and 6 nanometers in diameter, and they vary on the color they emit depending on their size.

Cyclical testing hasn't been done before, though many quantum dot papers have tried to expand the number of biomarkers tested for in a single cell. This method essentially reuses the same tissue sample, testing for biomarkers in groups of 10 in each round.

"Proteins are the building blocks for cell function and cell behavior, but their makeup in a cell is highly complex," Gao said. "You need to look at a number of indicators (biomarkers) to know what's going on."

The new process works like this: Gao and his team purchase antibodies that are known to bind with the specific biomarkers they want to test for in a cell. They pair quantum dots with the antibodies in a fluid solution, injecting it onto a tissue sample. Then, they use a microscope to look for the presence of fluorescent colors in the cell. If they see particular quantum dot colors in the tissue sample, they know the corresponding biomarker is present in the cell.

After completing one cycle, Gao and co-author Pavel Zrazhevskiy, a UW doctoral student in bioengineering, inject a low-pH fluid into the cell tissue that neutralizes the color fluorescence, essentially wiping the sample clean for the next round. Remarkably, the tissue sample doesn't degrade at all even after 10 such cycles, Gao said.

For cancer research and treatment, in particular, it's important to be able to look at a single cell at high resolution to examine its details. For example, if 99 percent of cancer cells in a person's body respond to a treatment drug, but 1 percent doesn't, it's important to analyze and understand the molecular makeup of that 1 percent that responds differently.

"When you treat with promising drugs, there are still a few cells that usually don't respond to treatment," said Gao. "They look the same, but you don't have a tool to look at their protein building blocks. This will really help us develop new drugs and treatment approaches."

The process is relatively low-cost and simple, and Gao hopes the procedure can be automated. He envisions a chamber to hold the tissue sample, and wire-thin pumps to inject and vacuum out fluid between cycles. A microscope underneath the chamber would take photos during each stage. All of the images would be quantified on a computer, where scientists and physicians could look at the intensity and prevalence of colors.

Gao hopes to collaborate with companies and other researchers to move toward an automated process and clinical use.

"The technology is ready," Gao said. "Now that it's developed, we're ready for clinical impacts, particularly in the fields of systems biology, oncology and pathology."


'/>"/>

Contact: Michelle Ma
mcma@uw.edu
206-543-2580
University of Washington
Source:Eurekalert  

Related biology technology :

1. New semiconductor research may extend integrated circuit battery life tenfold
2. Are Pros "Cheating" with Undetectable HGH Boosters?
3. Genuine Bio-Fuel Inc. Notes Sustainable Energy Gets A Much Needed Boost from the President Obama During 2013 Inaugural Address
4. Genedata Biologics 3.0 Boosts Efficiency Through Standardization
5. Tiny structure gives big boost to solar power
6. Nature Materials study: Boosting heat transfer with nanoglue
7. Industrial carbon management research gets $3.75 million boost
8. A 300 Million Euro Boost for Biomedicine in Berlin
9. Rice team boosts silicon-based batteries
10. LLSs Lead Therapy Acceleration Program in MDS Gets Boost from Major Industry Licensing Agreement
11. Spinach power gets a big boost
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Tenfold boost in ability to pinpoint proteins in cancer cells
(Date:2/11/2016)... 2016  Neurocrine Biosciences, Inc. (NASDAQ: NBIX ) today announced ... 2015. --> --> For ... of $29.3 million, or $0.34 loss per share, compared to a ... the same period in 2014. For the year ended December 31, ... $1.05 loss per share, as compared to a net loss of ...
(Date:2/11/2016)... NEW YORK , Feb. 11, 2016  Bioethics International, ... how medicines are researched, developed, marketed and made accessible to ... BMJ Open had named the publication of the ... for 2015. The publication is also featured as one of ... published in the last year that are most frequently read. ...
(Date:2/11/2016)... ... February 11, 2016 , ... ... dedicated to delivering cutting-edge information focused on the development and manufacture of ... become a premier sponsor of the 2016 BioProcess International Awards – Recognizing ...
(Date:2/11/2016)... BioInformant announces the February 2016 release of its ... and Technologies – Market Size, Segments, Trends, and Projections ... The first and only market research firm ... more than a decade of historical information on all ... type. This powerful 175 page global strategic report contains ...
Breaking Biology Technology:
(Date:2/2/2016)... NEW YORK , Feb. 2, 2016 ... healthcare facilities are primarily focused on medical ... that measure point-of-care parameters. Wearable devices that ... a user,s freedom of movement are being ... sensors for human biomedical signal acquisition coupled ...
(Date:2/2/2016)... NEW YORK , Feb. 2, 2016 /PRNewswire/ ... Potentials of that Rising Market Are you ... new analysis forecasts revenues for checkpoint inhibitors. Visiongain,s ... world market, submarket, product and national level. ... Instead discover what progress, opportunities and revenues those ...
(Date:2/2/2016)... , Feb. 2, 2016   Parabon NanoLabs ... the U.S. Army Research Office and the Defense ... and sensitivity of the company,s Snapshot Kinship ... Mission and, more generally, defense-related DNA forensics.  Although ... capabilities (predicting appearance and ancestry from DNA evidence), ...
Breaking Biology News(10 mins):