Navigation Links
Structure of enzyme against chemical warfare agents determined
Date:5/26/2009

This release is available in German.

The enzyme DFPase from the squid Loligo vulgaris, is able to rapidly and efficiently detoxify chemical warfare agents such as Sarin, which was used in the Tokyo subway attacks in 1995. A detailed understanding of the mechanism by which enzymes catalyze chemical reactions is necessary for efforts aiming to improve their properties. A group of researchers at the University of Frankfurt, the Bundeswehr Institute for Pharmacology and Toxicology in Munich, and Los Alamos National Laboratory in New Mexico, USA, have successfully determined the structure of DFPase using neutron diffraction. They report their findings in the 20 January 2009 issue of the journal Proceedings of the National Academy of Sciences (106(3), 713-718).

The team used the neutron source at Los Alamos National Laboratory, one of only three sources worldwide equipped for protein crystallography. In contrast to structure determination using X-rays, neutrons are able to locate the positions of hydrogen atoms, which make up half of all atoms in proteins, and are crucial for chemical reactions. As X-rays interact with the electron cloud around an atomic nucleus, so heavier elements are more easily seen, while neutrons interact with the atomic nuclei, and atoms in proteins such as hydrogen, oxygen, nitrogen, carbon, and sulfur, all scatter neutrons in a similar manner. Yet despite being so widespread, hydrogen atoms in proteins are quite elusive. As X-rays interact with the electron cloud around an atomic nucleus, hydrogen atoms, with only one electron, are normally invisible in structures. In contrast, neutrons interact with the atomic nuclei, such that atoms in proteins, hydrogen, oxygen, nitrogen, carbon, and sulfur, all scatter neutrons in a similar manner. The two techniques therefore yield complementary information about a protein structure. This information about hydrogen atoms is therefore essential for a basic understanding of the reaction mechanism of DFPase.

Neutron structures of proteins are quite rare and technically demanding, requiring large crystals and long measurement times. Though the first neutron structure of a protein was reported 40 years ago, in 1969, to date only about 20 unique structures have been solved, out of 50000 entries in the Protein Data Bank. " The effort has been absolutely worth it, " says Junior-Prof. Julian Chen, who published this work together with Dr. Marc-Michael Blum and Prof. Heinz Rueterjans. " Based on the results of this study, we can now create targeted changes to DFPase to augment the activity, as well as diversify the substrate range of the enzyme."


'/>"/>

Contact: Julian Chen
chen@chemie.uni-frankfurt.de
49-697-982-9641
Goethe University Frankfurt
Source:Eurekalert

Related biology technology :

1. Scientists determine the structure of highly efficient light-harvesting molecules in green bacteria
2. Structure of enzyme against chemical warfare agents determined
3. New security and medical sensor devices made possible by metallic nanostructures
4. ChemImage and Next Breath Structure Partnership to Validate Bioequivalence Testing for Nasal Spray Suspensions
5. Structure of enzyme against chemical warfare agents determined
6. Nanostructure boosts efficiency in energy transport
7. Cord Blood America Restructures Debt; Projects to be Cash Flow Positive
8. Sophisticated nano-structures assembled with magnets
9. New imaging technique reveals the atomic structure of nanocrystals
10. Engineers tune a nanoscale grating structure to trap and release a variety of light waves
11. Scientists prove graphenes edge structure affects electronic properties
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/27/2017)... , ... April 27, 2017 , ... ... for digital pathology, today announced their digital pathology technology has the potential to ... by five medical centers in The Netherlands as part of the 2017 ...
(Date:4/27/2017)... California (PRWEB) , ... April 27, 2017 , ... ... new infographic explaining why mass flow controllers based on capillary thermal mass flow ... industrial gas mass flow control applications. Over 80% of all industrial processes—such ...
(Date:4/27/2017)... -- Kinexum, a distinguished resource for research, development and commercialization ... Thomas C. Seoh as President and CEO. Mr. ... who becomes Executive Chairman and will continue to serve ... Thomas Seoh commented, "I am excited and ... the firm,s remarkable team of life science professionals, all ...
(Date:4/26/2017)... ... April 26, 2017 , ... Baltimore bio tech firm, ... security screening solution at the National Postal Forum 2017 in Baltimore, Maryland, May ... highly accurate, easy to use and low cost threat detection solution for government ...
Breaking Biology Technology:
(Date:4/3/2017)...  Data captured by IsoCode, IsoPlexis Corporation,s ... statistically significant association between the potency of ... objective response of cancer patients post-treatment. The ... cancer patients will respond to CAR-T cell ... to improve both pre-infusion potency testing and cell ...
(Date:3/30/2017)... The research team of The Hong Kong Polytechnic University ... adopting ground breaking 3D fingerprint minutiae recovery and matching technology, pushing ... for use in identification, crime investigation, immigration control, security of access ... ... A research team led by Dr Ajay ...
(Date:3/29/2017)... , March 29, 2017  higi, the health ... in North America , today announced ... and the acquisition of EveryMove. The new investment and ... set of tools to transform population health activities through ... lifestyle data. higi collects and secures data ...
Breaking Biology News(10 mins):