Navigation Links
Strange cousins: Molecular alternatives to DNA, RNA offer new insight into life's origins
Date:4/20/2012

operties. However, in order for any of these exotic molecules to have acted as a precursor to RNA in the pre-biotic epoch, they would need to have been able to transfer and recover their information from RNA. To do this, specialized enzymes, known as polymerases are required.

Nature has made DNA and RNA polymerases, capable of reading, transcribing and reverse transcribing normal nucleic acid sequences. For XNA molecules, however; no naturally occurring polymerases exist. So the group, led by Phil Holliger at the MRC in England, painstakingly evolved synthetic polymerases that could copy DNA into XNA and other polymerases that could copy XNA back into DNA. In the end, polymerases were discovered that transcribe and reverse-transcribe six different genetic systems: HNA, CeNA, LNA, ANA, FANA and TNA. The experiments demonstrated that these unnatural DNA sequences could be rendered into various XNAs when the polymerases were fed the appropriate XNA substrates.

Using these enzymes as tools for molecular evolution, the team evolved the first example of an HNA aptamer through iterative rounds of selection and amplification. Starting from a large pool of DNA sequences, a synthetic polymerase was used to copy the DNA library into HNA. The pool of HNA molecules was then incubated with an arbitrary target. The small fraction of molecules that bound the target were separated from the unbound pool, reverse transcribed back into DNA with a second synthetic enzyme and amplified by PCR. After many repeated rounds, HNAs were generated that bound HIV trans-activating response RNA (TAR) and hen egg lysosome (HEL), which were used as binding targets.) "This is a synthetic Darwinian process," Chaput says. "The same thing happens inside our cells, but this is done in vitro."

The method for producing XNA polymerases draws on the path-breaking work of Holliger, one of the lead authors of the current study. The elegant technique uses cell-like synthetic compar
'/>"/>

Contact: Richard Harth
richard.harth@asu.edu
Arizona State University
Source:Eurekalert  

Page: 1 2 3 4

Related biology technology :

1. Nanowrinkles, nanofolds yield strange hidden channels
2. Strange new twist: Berkeley researchers discover Möbius symmetry in metamaterials
3. GenScript Rush Gene Synthesis - Driving Molecular Biology Research Faster
4. Molecular graphene heralds new era of designer electrons
5. Molecular Detection Inc. Completes $1.5 Million Financing to Advance New Tests for Sepsis and GI Disorders
6. New England Biolabs Signs Agreement with Synthetic Genomics Inc. to Launch Gibson Assembly™ Master Mix for Molecular and Synthetic Biology Applications
7. Accelerate Brain Cancer Cure and Exosome Diagnostics Collaborate to Advance Clinical Studies of Exosome Biofluid Molecular Diagnostics Technology in Brain Cancer
8. Life Technologies Partners With DaAn Gene to Develop and Commercialize Molecular Diagnostic Assays in China
9. Amsterdam Molecular Therapeutics Receives US Orphan Designation for Hemophilia B Gene Therapy
10. Amsterdam Molecular Therapeutics Announces € 2.5 Million Equity Raise
11. The art of molecular carpet-weaving
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Strange cousins: Molecular alternatives to DNA, RNA offer new insight into life's origins
(Date:7/29/2014)... foam of atom-thick sheets can be made to any ... Rice University. , In microscopic images, the foam ... and walls that reinforce each other. The structure consists ... of graphene oxide that self-assemble with the assistance of ... the foam could find use in structural components, as ...
(Date:7/29/2014)... DALLAS , July 29, 2014 ... Market Slide Scanners (Whole Slide Imaging), Analytics (Image Analysis Software), ... Storage - Global Forecasts & Trends to 2018", published ... and restraints in geographies such as North ... Asia , and the Rest of the ...
(Date:7/29/2014)... , July 29, 2014  ImmunoClin Corporation (IMCL) ... medicine, treatment of infectious diseases as well as ... and prevention of pathologies like cardiovascular disease, Alzheimer,s ... 2014, ImmunoClin Corporation will complete the strategic relocation ... D.C. , a key center of North ...
(Date:7/29/2014)... LOUIS , July 29, 2014  Sigma-Aldrich Corporation ... water and energy use, increased its investment in Green ... service around the world last year, the Company announced ... What if Science Changed the World? ... earned several honors for being a responsible corporate citizen, ...
Breaking Biology Technology:Tough foam from tiny sheets 2Digital Pathology Market worth $437 Million by 2018 2Digital Pathology Market worth $437 Million by 2018 3ImmunoClin Corporation (IMCL) Relocates Corporate Headquarters to Washington, DC 2ImmunoClin Corporation (IMCL) Relocates Corporate Headquarters to Washington, DC 3Sigma-Aldrich Achieves Record Success in Environmental, Community Initiatives 2Sigma-Aldrich Achieves Record Success in Environmental, Community Initiatives 3
... up nearly 23% of fund raising goal for Central ... Company,(NYSE: LLY ) President and Chief Executive Officer ... United Way of Central Indiana for,its and other United ... of Lilly employees and retirees across the,United States with ...
... 13 Palatin Technologies,Inc. (Amex: PTN ) will ... Monday, November 17, 2008 before the open of the ... conference call and live audio webcast,hosted by Palatin,s executive ... discuss the results of operations in greater detail and ...
... 13 , - Announce Winners of ... Elsevier, the world,s leading research publisher of ... Institutions announced,today winners of the first annual ... to recognize outstanding young scientists and researchers,in ...
Cached Biology Technology:Lilly Contributes $11.1 Million to United Way Annual Campaign 2Palatin Technologies to Report Fiscal Year 2009 First Quarter Results; Conference Call and Webcast on November 17, 2008 2Elsevier and the Association of Southeast Asian Institutions 2Elsevier and the Association of Southeast Asian Institutions 3Elsevier and the Association of Southeast Asian Institutions 4
(Date:7/28/2014)... TEMPE, Ariz. - Reactions among minerals and organic compounds ... deep carbon cycle, they provide energy for the deep ... life. However, very little is known about how minerals ... Arizona State University have demonstrated how a common mineral ... negating the need for toxic solvents or expensive ...
(Date:7/28/2014)... The genome of Solanum pennellii , a wild ... an international group of researchers including the labs headed ... UC Davis Department of Plant Biology. The new genome ... , The work, published July 27 in the journal ... and colleagues at Aachen University in Germany. The UC ...
(Date:7/28/2014)... of the Earth that are high priorities for conservation ... particularly vulnerable, as it has the lowest fraction of ... continent in ,refugia, areas of biological diversity that ... constant during times of great environmental change. The refugia ... and Scotland. , The biggest refugia are in ...
Breaking Biology News(10 mins):Mineral magic? Common mineral capable of making and breaking bonds 2Mineral magic? Common mineral capable of making and breaking bonds 3Study finds Europe's habitat and wildlife is vulnerable to climate change 2
... 3, 2011 (Bronx, NY) People who live to ... of us in terms of their diet, exercise routine or ... Einstein College of Medicine of Yeshiva University., Their findings, ... the American Geriatrics Society , suggests that "nature" (in the ...
... growth factor was awarded more than 30 years ago, researchers ... University of Pittsburgh Professor Yadong Wang has developed a ... vessels. His research, which could be used to treat heart ... world, is published this week in the Aug. 1 issue ...
... Eggs, one of the most commonly consumed breakfast foods in ... they healthy or are they a high-cholesterol trap? The answer depends ... Dr. Niva Shapira of Tel Aviv University,s School of Health ... indicates that when hens are fed with a diet low in ...
Cached Biology News:Lifestyles of the old and healthy defy expectations 2Lifestyles of the old and healthy defy expectations 3Regrowing blood vessels with a potent molecule 2Regrowing blood vessels with a potent molecule 3Regrowing blood vessels with a potent molecule 4Can eggs be a healthy breakfast choice? 2