Navigation Links
Sneaking spies into a cell's nucleus
Date:9/28/2010

Institutes of Health.

The researchers coupled miniscule particles of silver, a metal that is not rejected by cells and is an efficient reflector of light, with a small portion of the HIV protein responsible for its highly efficient ability to enter a cell and its nucleus. In this case, the researchers harnessed only the ability of HIV to sneak past cellular defenses, while stripping away its ability to take over the cell's genetic machinery and cause disease.

"This combination takes advantage of the smallness of the nanoparticle and the 'delivery instructions' of the HIV protein," Gregas explained. "Once we can get that nanoparticle into the nucleus, we have many options. We can for example deliver some sort of payload and then observe its effects within the nucleus."

That's where a four-decades-old optical technique known as surface-enhanced Raman scattering (SERS) comes into play. It is used here as a sensitive imaging technique to demonstrate that the nanoparticles and their payloads successfully entered the nucleus.

When light, usually from a laser, is shined on a sample, the target molecule vibrates and scatters back its own unique light, often referred to as the Raman scatter. However, this Raman response is extremely weak. When the target molecule is coupled with a metal nanoparticle, the Raman response is greatly enhanced by the SERS effect -- often by more than a million times, Vo-Dinh said.

In the early 1980s, while at the Oak Ridge National Laboratory in Tennessee, Vo-Dinh and colleagues were among the first to demonstrate that SERS could be put into practical use to detect chemicals, including carcinogens, environmental pollutants and early markers of disease. At Duke, Vo-Dinh is pushing the boundaries of SERS technology for biomedical detection and molecular imaging.

"Our ultimate goal is to develop a nanoscale delivery system that can drop off its payload in this case nanoparticles with other agent
'/>"/>

Contact: Richard Merritt
richard.merritt@duke.edu
919-660-8414
Duke University
Source:Eurekalert  

Page: 1 2 3

Related biology technology :

1. Limbs saved by menstrual blood stem cells
2. ArunA Biomedical Announces Exclusive Worldwide Technology Licensing Agreement to Commercialize Human Embryonic Stem Cell Derived Mesenchymal Cells
3. UC Riverside researcher develops novel method to grow human embryonic stem cells
4. Stem cells stand up for themselves
5. Carnegie Mellon MRI technology that noninvasively locates, quantifies specific cells in the body
6. Researchers decode viral process that prepares cells for HIV infection
7. MaxCyte and Medinet to Present at the 10th International Symposium on Dendritic Cells in Kobe, Japan
8. Using living cells as nanotechnology factories
9. New properties of skin stem cells
10. Innovation and Strong Therapeutic Potential Seen in Adult Stem Cells, According to Frost & Sullivan
11. BioLife Solutions HypoThermosol(R) Adopted by MicroIslet for Processing Pancreatic Islet Cells to Treat Diabetes
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Sneaking spies into a cell's nucleus
(Date:11/18/2014)... 2014 PDL BioPharma, Inc. (PDL) (NASDAQ: ... the acquisition of Durata Therapeutics, Inc. by Actavis, PDL ... termination of PDL,s credit agreement with Durata, which included ... million, accrued interest, and prepayment and change of control ... financing transaction with Durata in which PDL agreed to ...
(Date:11/17/2014)... The new METTLER TOLEDO White Paper ... challenges of becoming the first calibration laboratory in ... weights in the microgram range. National Metrology Institutes ... scientists involved in nanotechnology applications will benefit from ... calibration of weights in the range of 0.05 ...
(Date:11/15/2014)... (PRWEB) November 13, 2014 Collaborative Consulting, ... they were named one of the Boston Globe’s “Top ... Medium Sized Businesses in Massachusetts to receive the honor. ... employers who take care of their most valuable resource: ... are presented annually, and are based on an array ...
(Date:11/15/2014)... November 14, 2014 CannLabs, ... proprietary, cloud-based analytics, and scientific testing methodologies relating ... third quarter ended September 30, 2014, and provided ... ,     CannLabs – Nevada, Inc. granted ... Nevada. ,     CannLabs – Connecticut, Inc. opened ...
Breaking Biology Technology:PDL BioPharma Announces Successful Conclusion of Debt Financing Agreement with Durata Therapeutics 2New White Paper on Microgram Weights Describes How to Achieve Traceable Measurement Down to 0.05 Milligrams 2Collaborative Consulting Honored with Boston Globe Top Places to Work 2014 2CannLabs Announces Third Quarter 2014 Results. 2CannLabs Announces Third Quarter 2014 Results. 3CannLabs Announces Third Quarter 2014 Results. 4CannLabs Announces Third Quarter 2014 Results. 5
... The Committee for,Medicinal Products for Human Use ... a negative opinion on a Cymbalta(R) (duloxetine,hydrochloride) application ... and Company and Boehringer Ingelheim are naturally,disappointed by ... director for duloxetine, Eli Lilly and Company. "We ...
... announced the availability of a 100% organic toxic mold remedy for ... based on the same technology used effectively in the aftermath of ... ... CA (PRWEB) October 24, 2008 -- Organic Environmental Technology ( http://www.organicet.com ...
... DIEGO, Oct. 23 Amylin Pharmaceuticals,Inc. (Nasdaq: AMLN ... in,San Diego on Tuesday, October 28, 2008 at 10:30 ... Finance and Chief Financial Officer, will,be providing a corporate ... and a recording will be made,available following the event. ...
Cached Biology Technology:European CHMP Adopts Negative Opinion on Cymbalta for the Treatment of Fibromyalgia 2European CHMP Adopts Negative Opinion on Cymbalta for the Treatment of Fibromyalgia 3European CHMP Adopts Negative Opinion on Cymbalta for the Treatment of Fibromyalgia 4European CHMP Adopts Negative Opinion on Cymbalta for the Treatment of Fibromyalgia 5Katrina Proven Certified Organic Toxic Mold Remedy Now Available for Mold Remediation Professionals and Homeowners 2Katrina Proven Certified Organic Toxic Mold Remedy Now Available for Mold Remediation Professionals and Homeowners 3Katrina Proven Certified Organic Toxic Mold Remedy Now Available for Mold Remediation Professionals and Homeowners 4
(Date:11/4/2014)... 2014   Neurotechnology , a provider of ... latest version of its fingerprint matching algorithm has ... evaluation organized by NIST. The MINEX test ... INCITS 378 fingerprint standard template format. MINEX compliance ... in the United States ...
(Date:11/3/2014)... 3, 2014  NeuroSigma ® , Inc. (NeuroSigma), ... company focused on commercialization of its non-invasive ... treatment of neurological and neuropsychiatric disorders, announced today ... issued a Notice of Allowance for Mexican Patent ... by NeuroSigma and the Regents of the University ...
(Date:11/2/2014)... LA (2 November 2014)—In support of a bold quest ... & Melinda Gates Foundation today announced an award of ... Vaccine Initiative (MVI) in building new vaccines that will ... realize the "accelerating to zero" agenda. Such vaccines would ... could be called an "immunological bed net." , ...
Breaking Biology News(10 mins):Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 2Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 3NeuroSigma Receives Notice of Allowance from the Mexican Institute of Industrial Property Covering Subcutaneous Trigeminal Nerve Stimulation (sTNS) 2NeuroSigma Receives Notice of Allowance from the Mexican Institute of Industrial Property Covering Subcutaneous Trigeminal Nerve Stimulation (sTNS) 3New malaria vaccines to prevent infection and block transmission get a shot in the arm 2New malaria vaccines to prevent infection and block transmission get a shot in the arm 3New malaria vaccines to prevent infection and block transmission get a shot in the arm 4
... Scientists at Stanford University have developed a new laser ... unharmed. The new, non-invasive treatment is described in a ... the Proceedings of the National Academy of Sciences (PNAS). ... how to cure cancer without harming normal body tissue," ...
... from sensory neurons using a code more complicated than ... National Autonomous University of Mexico and Cold Spring Harbor ... when it touches the skin, scientists found that changes ... sensory signal is interpreted. , Howard Hughes Medical Institute ...
... looking deep under water for clues on how to ... Some of the stresses inherent with travel and life ... example - are not easily remedied with traditional plant ... Distinguished Professor of Botany, and Dr.Amy Grunden, assistant professor ...
Cached Biology News:Scientists develop nanotech-laser treatment that kills cancer cells without harming healthy tissue 2Scientists develop nanotech-laser treatment that kills cancer cells without harming healthy tissue 3Scientists develop nanotech-laser treatment that kills cancer cells without harming healthy tissue 4Cracking the perception code 2Cracking the perception code 3NC State researchers redesign life for Mars and beyond 2
... polyacrylamide gel electrophoresis (2D-PAGE) with biological mass ... technology platform for proteomic analysis. 2D-PAGE gels ... limited only in the dynamic range and ... Quantitative image based analysis of separated proteins ...
... The BV-10 offers precision ... 0.1 and 50m. The unique ... vibration free for greater control ... can be accomplished very rapidly ...
... EC570-90 is a compact, lightweight, high current power ... or 2500 milliamps. It is an excellent choice ... gels. It is also ideal for all types ... be set to automatically end a run and ...
... is a highly purified preparation qualified to ... vectors. The preparation is functionally tested for ... of detectable contaminating proteases. Prepared from human ... tests to be negative for HBsAg and ...
Biology Products: