Navigation Links
Single-molecule technique captures calcium sensor calmodulin in action
Date:8/14/2009

ief says, "is that we can work directly in aqueous solution. We can make our measurements in exactly the conditions under which the protein works in its natural environment. So we can directly observe how the calmodulin snatches the amino acid chain and folds itself, to hold its target fast." Measuring the force needed to bend the calmodulin molecule out of its stable condition at any given moment enables the researchers to compute the energies associated with binding both the calcium ions and the amino acid chains. And by following changes in the molecule's mechanical properties over time, they also can determine how long a protein fragment remains bound.

The results Rief and biophysicist Jan Philipp Junker report in the early edition of PNAS show that their approach also enables detailed comparative studies of binding sequences for different target proteins. The target sequences observed in these experiments are called skMLCK and CaMKK. Rief and Junker used mechanical force actually pulling on complexes of calmodulin and the target peptides at rates of 1 nanometer per second or less to slow down the processes to observable time scales and to clearly separate the individual unbinding steps.

"By applying mechanical force," Junker says, "we are able to dismantle the calmodulin-target peptide complex with surgical precision. Using conventional methods, this would be very difficult to do."

Among the detailed insights this approach made accessible are the hierarchy of folding and target binding, the sequence of unbinding events, and target-specific differences in terms of what is called cooperative binding.


'/>"/>

Contact: Patrick Regan
regan@zv.tum.de
49-892-892-2743
Technische Universitaet Muenchen
Source:Eurekalert  

Page: 1 2

Related biology technology :

1. BioNanomatrix Awarded New NIH Grant for Development of Nanoscale Platform for Single-Molecule DNA Mapping and Haplotype Applications
2. Model for the assembly of advanced, single-molecule-based electronic components developed at Pitt
3. New MRI technique could mean fewer breast biopsies in high-risk women
4. New statistical technique improves precision of nanotechnology data
5. Five Cord Blood Transplant Centers in Catalonia and Valencia Join a Study to Test a New Technique to Treat Leukemia and Lymphoma
6. Secret of sandcastle construction could help revive ancient building technique, researchers say
7. Quest Diagnostics to Present Genomic Studies Related to New Testing Techniques for Leukemia and Prostate Cancer at 2009 ASCO Meeting
8. Gender-Selection Technique Embraced for Hopeful Parents
9. Novel CU-Boulder technique shrinks size of nanotechnology circuitry
10. Hebrew University scientist develop technique for eliminating reblockage of arteries
11. New imaging technique reveals the atomic structure of nanocrystals
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Single-molecule technique captures calcium sensor calmodulin in action
(Date:8/28/2014)... --  SunTrust Robinson Humphrey (STRH) today announced ... equity research team in biotechnology and oilfield services.  ... research demonstrates our commitment to providing our valued ... investment decision making," said Biff Woodruff , ... make significant investments in differentiated content at a ...
(Date:8/28/2014)... 2014 A new market research ... is available from Strategic Consulting, Inc., the leading ... Microbiology Market Review, Fourth Edition: Global Review of ... tracks and compares microbiology test volumes, market values ... and Asia, and forecasts future volumes and market ...
(Date:8/27/2014)... 27, 2014 Varian Medical Systems today ... current Salt Lake City manufacturing facility and allow for ... to ultimately create 1,000 new full time jobs in ... “Utah is home to hardworking people with immense engineering ... with Varian’s success in the manufacturing of our state-of-the-art ...
(Date:8/27/2014)... Aug. 27, 2014   MSC , a healthcare ... today announced the appointment of Mary Beth Loesch ... 28 years of experience preparing companies for rapid growth ... Executive Vice President of Corporate Development and Healthcare. In ... business , as well as corporate strategy and marketing. ...
Breaking Biology Technology:SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 2SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 32 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 3Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 2Varian Medical Systems Groundbreaking on Facility Expansion Triggers Influx of New Jobs to Salt Lake 3MSC names Mary Beth Loesch President and CEO 2
... launched its revolutionary Trusted LASIK Surgeons directory service and ... a resource for consumers seeking to improve their vision ... improve their chances of attaining 20-20 vision. What ... unique screening process ensuring that only those who are ...
... GridGain System partnered up in delivering industry first dedicated solution for ... integration between GridGain,s Open Cloud Platform and Inventigo,s FastXML product provides ... real-time fashion. , ... San Francisco, CA (PRWEB) February ...
... EdmontonThe University of Alberta and the National Research Council,s ... that is leading to improved performance of plastic solar ... mass-produced plastic solar panels is a goal of intense ... because of the high cost and shortage of the ...
Cached Biology Technology:Rick Fraunfelder, M.D. of the Casey Vision Correction Center in Portland, Oregon, Joins Elite Group of Surgeons Listed at Trusted LASIK Surgeons 2Rick Fraunfelder, M.D. of the Casey Vision Correction Center in Portland, Oregon, Joins Elite Group of Surgeons Listed at Trusted LASIK Surgeons 3Rick Fraunfelder, M.D. of the Casey Vision Correction Center in Portland, Oregon, Joins Elite Group of Surgeons Listed at Trusted LASIK Surgeons 4Rick Fraunfelder, M.D. of the Casey Vision Correction Center in Portland, Oregon, Joins Elite Group of Surgeons Listed at Trusted LASIK Surgeons 5Rick Fraunfelder, M.D. of the Casey Vision Correction Center in Portland, Oregon, Joins Elite Group of Surgeons Listed at Trusted LASIK Surgeons 6Rick Fraunfelder, M.D. of the Casey Vision Correction Center in Portland, Oregon, Joins Elite Group of Surgeons Listed at Trusted LASIK Surgeons 7Rick Fraunfelder, M.D. of the Casey Vision Correction Center in Portland, Oregon, Joins Elite Group of Surgeons Listed at Trusted LASIK Surgeons 8Inventigo and GridGain Systems Team Up on Delivering Cloud-Enabled High Performance XML Processing 2University of Alberta and NINT researchers make solar energy breakthrough 2
(Date:8/29/2014)... German . ... the oldest form of communication. Acting as messenger molecules, ... sexual attraction between males and females. Fish rely on ... behavior in males and females. Scientists at the Marine ... Faro, Portugal, and at the Max Planck Institute for ...
(Date:8/29/2014)... them, twist them, fold them: modern materials that are ... whether as artificial skin or electronic paper. , Making ... challenge but a new way of working with copper ... game-changer. , Previous success in the field of ... of precious gold and silver nanowires. , By ...
(Date:8/28/2014)... pleased to announce the selection of Dr. Fred Baxendale ... acknowledges those who have served ESA for at least ... the Society that has reached an extraordinary level. Candidates ... Board and then voted on by the ESA membership. ... at Entomology 2014, ESA,s Annual Meeting in Portland, Oregon ...
Breaking Biology News(10 mins):Ready for mating at the right time 2Ready for mating at the right time 3Copper shines as flexible conductor 2Fred Baxendale selected as Honorary Member of the Entomological Society of America 2
... against infection. But people with a rare, life-threatening autoimmune ... immune system attacks the proteins that hold skin cells ... the top layer of their skin falls apart, leaving ... of drugs that completely suppress the immune system offered ...
... Whether or not a fertilized mammalian egg ultimately develops into ... a tug of war between two different genes encoding signaling ... new study led by Duke University Medical Center cell biologists. ... two specific genes, Wnt4 and Fgf9, are in equal balance ...
... By training a group of human subjects to operate a ... the brain "learns" to control certain muscle movements, the more ... haul. The results, the investigators say, could alter rehabilitation approaches ... like strokes. , In a report on the work ...
Cached Biology News:Scientists stop autoimmune disease without shutting off immune system 2Genetic tug of war determines sexual differentiation 2Genetic tug of war determines sexual differentiation 3New approach to vaccine development provides potent, long-lasting immunity 2New approach to vaccine development provides potent, long-lasting immunity 3
... reagent is formulated specifically for Alkaline Phosphatase ... levels of activity for both the enzyme ... final dilution. This product has been ... the customer a longer shelf life, resistance ...
... When nonspecific binding is a ... a variety of immobilized NeutrAvidin products ... streptavidin. NeutrAvidin is a modified avidin ... to provide a biotin-binding protein with ...
... The Sciclone ALH 4000 workstation is the ... seeking to introduce automated liquid handling capabilities,into ... that need to support routine applications on ... and,integrated for walk-away automation even when ...
... Service ECACC is able to accept cell lines ... cryo-storage. The cell lines must be accompanied by ... in accordance with the requirements of cGMP and ... mycoplasma, bacteria or fungi. What are the benefits ...
Biology Products: