Navigation Links
Sigma-Aldrich Introduces MISSION(R) esiRNA for Low Cost, High Specificity Pooled RNAi Screening
Date:4/28/2009

ST. LOUIS, April 28 /PRNewswire-FirstCall/ -- Sigma-Aldrich (Nasdaq: SIAL) today announced the global release of MISSION(R) esiRNA, a pool of gene-specific siRNA that provides a novel and powerful approach to RNAi screening in mammalian cells. Unlike traditional synthetic RNAi, esiRNA (http://www.sigma.com/esirna) incorporates a pool of hundreds of siRNAs against a single gene target. This new tool is designed to eliminate the trial and error approach of identifying a single siRNA for gene knockdown and ensures minimal off-target effects.

"MISSION esiRNA provides a powerful way to elucidate gene function in cell culture assays," said Dr. Supriya Shivakumar, global marketing manager for Functional Genomics at Sigma-Aldrich. "The pooled format, unlike anything else on the market, should enable researchers to knock down genes rapidly and cost-effectively. This is an exciting addition to our current MISSION lineup and provides an effective technology that will facilitate RNAi research for a broad base of scientists."

The technique offers a number of benefits over the traditional approach to single-siRNA gene knockdown. By targeting each gene target with a "super-pool" of siRNA, the trial and error approach of identifying a useful single siRNA is eliminated. In addition, the approach should ensure minimal risk of off-target effects and enables the use of one assay per gene. Development of the esiRNA approach was supported by the German BMBF program "GO-Bio" and developed by the Max Planck Institute for Cell Biology and Genetics (MPI-CBG) in Dresden, Germany.

Frank Buchholz, a research group leader at the MPI-CBG, has pioneered the use of the esiRNA technology in RNAi screens. "We are delighted to be partnering with Sigma-Aldrich to make esiRNA technology available to the research community," he said. "esiRNAs were invented in Mike Bishop's laboratory at UCSF almost 10 years ago. esiRNA has been a major research focus of my group ever since. Sigma-Aldrich's extensive experience and product range in RNAi are a perfect complement to our effort to produce efficient and specific esiRNAs."

Harvard Medical School, which has invested in a human genome-wide esiRNA library, is using esiRNA to help identify the human genes that enable HIV propagation. "The phenotypic results from the initial tests of MISSION esiRNA using our assay for finding HIV dependency factors demonstrated very effective knockdown over a wide range of genes," said Dr. Abraham Brass, a clinical research fellow at Harvard Medical School.

Harvard Medical School will soon incorporate the technology into a wider range of tests. Dr. Caroline Shamu, director of the ICCB-Longwood Screening Facility, which provides high-throughput screens of RNAi libraries for Harvard Medical School researchers, said, "We are looking forward to screening the MISSION esiRNA library at ICCB-Longwood."

esiRNA are endoribonuclease-prepared siRNAs synthesized by in vitro transcription of a 300-600-bp gene specific double-stranded RNA, followed by enzymatic digestion. This collection of siRNA-like molecules is then purified, resulting in a complex pool of siRNA molecules all targeting different sequences of a single gene.

For more information on MISSION(R) esiRNA, please visit www.sigma.com/esirna.

About Sigma-Aldrich: Sigma-Aldrich is a leading Life Science and High Technology company. Its biochemical and organic chemical products and kits are used in scientific research, including genomic and proteomic research, biotechnology, pharmaceutical development and as key components in pharmaceutical, diagnostic and other high technology manufacturing. The Company has customers in life science companies, university and government institutions, hospitals, and in industry. Over one million scientists and technologists use its products. Sigma-Aldrich operates in 38 countries and has 7,800 employees providing excellent service worldwide. Sigma-Aldrich is committed to Accelerating Customer Success through Innovation and Leadership in Life Science, High Technology and Service. For more information about Sigma-Aldrich, please visit its award-winning Web site at http://www.sigma-aldrich.com.

About Max Planck Institute: The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) is one of 80 institutes of the Max Planck Society, an independent, non-profit organization in Germany. MPI-CBG was founded in 1998 and since February 2001, scientists from over 40 nations have been working under one roof. The Institute has a core staff of about 400 scientists, which form a network of 27 research groups covering different topics at the interface of cell biology and developmental biology including research investigating illnesses such as cancer or Alzheimer's disease. For example, at the MPI-CBG researchers study how the growth of cells is controlled and why this control process fails to function properly in cancer cells. Once science has an understanding of how cellular control systems work, currently incurable illnesses may be diagnosed earlier with a view to developing more effective treatments.

The MPI-CBG has forged partnerships with technology providers in many of its major areas of research since it believes that the greatest insight and new discoveries are made when innovations in new technologies allow problems in research to be addressed or investigated in new ways. For more information about the Max Planck Institute, please visit www.mpi-cbg.de.

Cautionary Statement: This release contains forward-looking statements relating to future strategic actions and initiatives and similar intentions and beliefs and other statements regarding the Company's expectations, beliefs, intentions and the like, which involve assumptions regarding the Company's operations and conditions in the markets the Company serves. The Company does not undertake any obligation to update these forward-looking statements.

Sigma-Aldrich and MISSION are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.


'/>"/>
SOURCE Sigma-Aldrich Corp.
Copyright©2009 PR Newswire.
All rights reserved

Related biology technology :

1. Sigma-Aldrich (Nasdaq: SIAL) Declares Quarterly Dividend
2. Sigma-Aldrich and Lab Manager Magazine Present Webinar On Laboratory Environmental, Health and Safety Compliance Strategies
3. Sigma-Aldrich Expansion in Republic of China to Support SAFC Growth
4. Sigma-Aldrich and Ingenuity Systems Announce Partnership to Create E-Commerce Search Solution Based on Biological Knowledge
5. Sigma-Aldrich to Present at the UBS 2007 Global Life Sciences Conference on September 25, 2007
6. Sigma-Aldrich Names Patrick Sullivan New VP of Research and Development for Research Biotech
7. Sigma-Aldrich and the Universite de Montreal Establish Collaboration for RNA Interference Studies
8. Sigma-Aldrich Corporations 3rd Quarter 2007 Earnings Conference Call
9. Sigma-Aldrich Introduces N-TER Nanoparticle siRNA Transfection System for Delivery of siRNA Into Difficult-to-Transfect Cell Types
10. Sigma-Aldrich (Nasdaq: SIAL) Q3 2007 Sales Increase 14.0%, Yielding Diluted EPS of $.54.
11. Sigma-Aldrich and Oxford BioMedica Win Key Ruling in Open Biosystems Patent Infringement Dispute
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/5/2016)... ... May 05, 2016 , ... American ... two additional patents, U.S. Patent Nos. 9,322,133 and 9,322,134, to API and its ... nanocellulose as well as hydrophobic nanocellulose compositions. In addition to these patents ...
(Date:5/5/2016)... BELL, Pa. , May 5, 2016  Why ... Powers and Lorna Weir launching a ... un-agency way? Because they believe that truly helping clients ... world of healthcare now demands a different type of ... strategy lab serving the pharmaceutical, biotechnology and medical device ...
(Date:5/5/2016)... , ... May 05, 2016 , ... ... previously announced identification of its first three targets, it has identified a fourth ... beta (Aß), implicated in the development and progression of Alzheimer’s disease (AD). ...
(Date:5/4/2016)... ... 2016 , ... CereScan, the nation’s leader in providing statistically ... Stroke Awareness Month in May. An infographic created by CereScan will be ... will donate $1 up to a maximum of $3,000 through users who share ...
Breaking Biology Technology:
(Date:5/3/2016)... 2016  Neurotechnology, a provider of high-precision biometric ... Biometric Identification System (ABIS) , a complete system ... ABIS can process multiple complex biometric transactions with ... fingerprint, face or iris biometrics. It leverages the ... MegaMatcher Accelerator , which have been used ...
(Date:4/28/2016)... and BANGALORE, India , April 28, ... Systems, a product subsidiary of Infosys (NYSE: INFY ... announced a global partnership that will provide end ... use mobile banking and payment services.      (Logo: ... key innovation area for financial services, but it also plays ...
(Date:4/28/2016)... , April 28, 2016 First quarter ... (139.9), up 966% compared with the first quarter of 2015 ... totaled SEK 589.1 M (loss: 18.8) and the operating margin was ... (loss: 0.32) Cash flow from operations was SEK 249.9 ... 2016 revenue guidance is unchanged, SEK 7,000-8,500 M. The ...
Breaking Biology News(10 mins):