Navigation Links
Sigma® Life Science Creates World's First Knock-in Rats using Zinc Finger Nuclease Technology
Date:12/13/2010

ST. LOUIS, Dec. 13, 2010 /PRNewswire-FirstCall/ -- Sigma Life Science, the innovative biological products and services business of Sigma-Aldrich® (Nasdaq: SIAL), today announced that its SAGE Labs initiative has successfully used its proprietary CompoZr® Zinc Finger Nuclease (ZFN) technology to generate knock-in rats in which foreign genes have been inserted, or 'knocked-in', into the rat genome in a precisely targeted manner.  This breakthrough achievement represents a major step forward in the creation of a transgenic animal, which may serve as more predictive models of human disease.

In a proof of concept study conducted by researchers at SAGE Labs, a copy of the green fluorescent protein (GFP) was inserted in a targeted fashion into the Mdr1a gene of a rat, thus creating a rat that glows under special lighting conditions.  The study, which has recently been accepted for publication(1), opens the doors to a variety of new applications including the tagging of genes with reporters such as GFP to measure gene expression patterns and replacement of rat genes with humanized versions.

Although rats have long been the model organism of choice for disease research, scientists have been forced to use mouse models for over a quarter of a century, due to their amenability to genetic manipulation. The creation of rats with gene deletions, commonly called 'knock-outs', using ZFN technology was first published in Science in 2009(2), and the ability to create rats with new genes introduced in their genome represents a leap forward in ZFN technology, allowing sophisticated genetic engineering of higher level organisms and providing scientists with a choice of model organisms that can be used to investigate different diseases.

Sigma Life Science's CompoZr® ZFN technology is the only technique that has repeatedly been shown to make highly targe
'/>"/>

SOURCE Sigma-Aldrich
Copyright©2010 PR Newswire.
All rights reserved

Page: 1 2 3 4

Related biology technology :

1. Sigma® Life Science Awarded Silver in Top Ten Innovations 2010
2. Sigma® Life Science Enters Genetically Modified Cell Market
3. Pacific Biosciences and Harvard scientists decode genome of Haitian cholera pathogen
4. Ramius Offers to Enter Into Immediate and Exclusive Negotiations to Acquire Cypress Bioscience for $5.50 Per Share
5. Medical science helps UK athletes reach peak performance
6. The Biotechnology Industry Organization and Critical Path Institute Partner With Mediaplanet, Biocom, and the University of California Office to Spotlight the Science That Is Healing, Fueling and Feeding Our Global Population
7. AltheaDx and Compendia Bioscience Launch the Breast Cancer Segregation Panel™
8. Sangamo BioSciences Announces Data Presentation of First In Vivo Demonstration of ZFN-mediated Gene Correction Via Systemic Delivery at American Society for Hematology Meeting
9. YM BioSciences Reports Positive Interim Data Including Substantial Anemia Response from Phase I/II Trial of JAK1/JaK2 Inhibitor CYT387
10. Egon Zehnder International Names Todd Hutchings to Lead Life Sciences Practice Group
11. The Pittsburgh Life Sciences Greenhouse Announces New Chairman of the Board
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/22/2015)... NH (PRWEB) January 22, 2015 GEA ... a table top laboratory homogenizer, the PandaPLUS 2000, which ... nanodispersions, nanoemulsions , and cell disruption . This ... dairy products, fruit juices, liquid food, food additives and ...
(Date:1/22/2015)... (PRWEB) January 21, 2015 Cambridge Semantics, the ... technology, today announced that 2014 was a record-breaking year across ... capabilities of the Anzo Smart Data Platform and our Smart ... utilize insights from diverse data which led to record growth ...
(Date:1/22/2015)... RI (PRWEB) January 22, 2015 Protocol ... , The growing team of brand-neutral, independent consultants, Protocol ... Philip Rogers explained that over the years, his company ... , Moroso, Town of Plainfield, as well as others. ...
(Date:1/22/2015)... January 22, 2015 Pipette.com has added ... to their comprehensive portfolio of Eppendorf products. , The ... purchase a Centrifuge 5424/5424 R and receive the following:, ... Eppendorf Research plus or Eppendorf Reference 2 , ...
Breaking Biology Technology:GEA Niro Soavi Announces the PandaPLUS 2000 Homogenizer for Processing New Applications for Nanoemulsions and Cell Disruption 2Cambridge Semantics Announces Record Results for 2014 2Protocol Networks Brings Independent Technology Consulting to the Constitution State 2Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2
... Lisa Marsh Carsten Carstens ,Stratagene , ... expression ,vectors offers unparalleled convenience. The drug-resistance ... without subcloning. Through site-specific ,recombination, hygromycin, puromycin, ... efficiently inserted into the pExchange core vectors. ...
... LipoTAXI transfection reagent: A proven ... ,Stratagene , LipoTAXI ... cells and cells growing in,suspension. In this article, ... the effectiveness of LipoTAXI reagent. Optimizing transfection,conditions is ...
... of 96 PCR products , , Scott ... , The StrataPrep 96 PCR purification ... This kit offers a high-throughput format,for purifying ultra-pure PCR ... PCR purification kit uses the same silica matrix-binding,technology as ...
Cached Biology Technology:Mammalian Expression Vectors with Interchangeable Selectable Markers 2Mammalian Expression Vectors with Interchangeable Selectable Markers 3Mammalian Expression Vectors with Interchangeable Selectable Markers 4Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 2Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 3Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 4Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 5Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 6Versatile Transfection Reagent Offers Low Toxicity and Consistent,Performance 7High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,Applications 2High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,Applications 3High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,Applications 4High-Throughput System Generates Ultra-Pure PCR Products Suitable for All,Applications 5
(Date:1/22/2015)... Technology Showcase, Hall E -   EyeLock Inc. , ... it will showcase its EyeLock ID technology integrated in a ... Energy,s Oak Ridge National Laboratory (ORNL) at the ... identity authentication technology is being used to validate the driver ...
(Date:1/22/2015)... Washington , January 20, 2015 Investorideas.com, ... tech stocks, releases video from the CES 2015 (Consumer Electronics ... Apollo Robbins . Apollo shows how easily ... smart wallet, a product of NXT-ID, Inc. (NASDAQ: ...
(Date:1/22/2015)... EyeLock, Inc. , a market leader of iris-based identity authentication ... to the new role of Senior Vice President of Mobile ... mobile platforms and wearable solutions for EyeLock,s technologies. Gerber brings ... semiconductor industry to his role at EyeLock, with expertise in ...
Breaking Biology News(10 mins):EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 3Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 3EyeLock Names Steve Gerber as Senior Vice President of Mobile and Wearables 2
... researchers have demonstrated a close correlation between the decline ... system and the progression of chronic obstructive pulmonary disease ... decreases the flow of air through the lungs as ... lung tissue samples from COPD patients by scientists at ...
... Media,Group,s, C4ISR Journal will host its 8th Annual C4ISR ... October 16-17, 2008 at the Sheraton,Crystal City Hotel, Arlington, ... C4ISR technology through,various conference sessions including:, ... Sharing for Homeland Defense/Civil Support -- From Net-Centric ...
... MANHATTAN, KAN. -- New findings about the causes of ... genetic. "We now know it,s also in the ... Professor of Diagnostic Medicine and Pathobiology at Kansas State ... ago, Richt said, it was thought that the cattle ...
Cached Biology News:Key component of debilitating lung disease identified 2Annual C4ISR Integrated Conference and Big 25 Awards Banquet 2Annual C4ISR Integrated Conference and Big 25 Awards Banquet 3K-State professor's USDA research shows mad cow disease also caused by genetic mutation 2
TP-1 (NDOG1)...
Mouse monoclonal [6G11] to Cytohesin 1 + 2 ( Abpromise for all tested applications)....
HP 1 alpha Immunogen: Synthetic peptide between amino acids 1-100 of the human HP 1 alpha protein. Storage: -20 C, Avoid Freeze/Thaw Cycles...
... Immunogen KLH-conjugated, synthetic ... 54-75 (RRGPHRNPG-PGDDDGQRSRSSS) of mouse p19ARF. ... to amino acids 62-75. Clone ... Routinely evaluated by immunoblot. ...
Biology Products: