Navigation Links
See-through organs and bodies will accelerate biomedical discoveries
Date:7/31/2014

The ability to see through organs and even the entire body to visualize long-range connections between cells as well as fine-grained cellular structures has been a long-time dream of biologists. A study published by Cell Press July 31st in the journal Cell has now made that dream a reality, revealing simple methods for making opaque organs, bodies, and human tissue biopsies transparent, while keeping the cellular structures and connections intact. The protocols could pave the way for a better understanding of brain-body interactions, more accurate clinical diagnoses and disease monitoring, and a new generation of therapies for conditions ranging from autism to chronic pain.

"Although the idea of tissue clearing has been around for a century, to our knowledge, this is the first study to perform whole-body clearing, as opposed to first extracting and then clearing organs outside the adult body," says senior study author Viviana Gradinaru of the California Institute of Technology. "Our methodology has the potential to accelerate any scientific endeavor that would benefit from whole-organism mapping, including the study of how peripheral nerves and organs can profoundly affect cognition and mental processing, and vice versa."

Three-dimensional maps of intact organs and bodies are crucial for understanding complex, long-distance cellular interactions that play an important role in a range of biological processes. But until now, methods for making whole organs or bodies transparent and thus amenable to imaging and generating 3D maps have been limited to the brain or embryos. Gradinaru and her collaborators previously developed a brain-clearing technique called CLARITY, which involves embedding tissue into hydrogels to preserve its 3D structure and important molecular features, and then using detergents to extract lipids that make the tissue opaque.

In the new study, the researchers set out to make CLARITY suitable for whole organs and bodies, in part by making the process faster. First, they identified the optimal hydrogel that allows detergents to quickly remove lipids from tissue using an approach named passive CLARITY technique (PACT). To markedly speed up the clearing process without causing tissue damage, they introduced an innovative procedure called perfusion-assisted agent release in situ (PARS), which involves directly delivering the hydrogel and clearing reagents into the bloodstream of intact rodents. The reagents diffused throughout the tissues and completely clarified organs such as the kidney, heart, lung, and intestine within 2 to 3 days, while the whole brain and entire body cleared within 2 weeks.

The researchers also developed a recipe for refractive index matching solution (RIMS), which enables the long-term storage of cleared tissue and imaging thick, cleared tissue using a conventional confocal microscope. The new methods allow for the analysis of intact connections between cells as well as structures and molecules within single cells using standard genetic and molecular biology techniques. "Our easy-to-use tissue clearing protocols, which employ readily available and cost-effective reagents and equipment, will make the subcellular interrogation of large tissue samples an accessible undertaking within the broader research and clinical communities," Gradinaru says.


'/>"/>

Contact: Mary Beth O'Leary
moleary@cell.com
617-397-2802
Cell Press
Source:Eurekalert  

Related biology technology :

1. Dr. Rongxiang Xu to Reveal How Human Organs can Achieve Self-Regeneration during His Acceptance of the International Golden Biatec 2013 Award
2. Damaged Tissues and Organs Could be Repaired With New Scaffolding Technique
3. Dr. Rongxiang Xu, Owner of Patented Technology to Regenerate Damaged Organs, Announces Lawsuit Against 2012 Nobel Laureate Dr. Shinya Yamanaka
4. Findings could be used to engineer organs
5. Engineered anti-toxin antibodies improve efficacy
6. Monoclonal Antibodies Market in Colorectal Cancer to 2019
7. Industry Shares, Size, Trend, Analysis, and Forecasts Report Available Online by Researchmoz.us
8. The Engineered T-Cell Receptor in Fusion Proteins, Antibodies & Cells: Emerging Opportunities for the Biopharmaceutical Industry
9. NKT Therapeutics Identifies Novel anti-iNKT Antibodies for Depleting or Activating iNKT Cells
10. Monoclonal Antibodies Market in Breast Cancer to 2019
11. Identifying targets of autoantibodies
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
See-through organs and bodies will accelerate biomedical discoveries
(Date:1/12/2017)... Jan. 12, 2017   Protein Sciences Corporation ... of Flublok Influenza Vaccine ®, announced today ... had good safety results and induced strong neutralizing ... The product is expected to advance into human ... addition, the Institute of Technology in Immunobiologicals of ...
(Date:1/12/2017)... (PRWEB) , ... January 12, ... ... market’s rapid adoption of Limfinity® Cloud, RURO has enhanced the platform to ... user groups, federated login, rapid data searching, and more. In addition to ...
(Date:1/11/2017)... ... January 11, 2017 , ... As ... in Peru studying the pathogens that cause malaria and tuberculosis. Seeing firsthand the ... of discovery. , Now, as an assistant professor of biology and biotechnology at ...
(Date:1/11/2017)... Linda, Ca (PRWEB) , ... January 11, 2017 ... ... the U.S. each year and costing healthcare systems more than $23.7 billion, ... while controlling costs. , Among the most common sepsis-causing pathogens are bacteria ...
Breaking Biology Technology:
(Date:1/11/2017)... Jan. 11, 2017  Michael Johnson, co-founder of Visikol Inc. a ... Inc., has been named to the elite "Forbes 30 Under 30" ... of 600 people in 20 fields nationwide to be recognized as ... 15,000 applicants were selected. ... He is currently a PhD candidate at Rutgers University. ...
(Date:1/6/2017)... 2017  Delta ID Inc., a leader in consumer-grade ... automotive at CES® 2017. Delta ID has collaborated with ... the use of iris scanning as a secure, reliable ... in a car, and as a way to elevate ... Delta ID and Gentex will demonstrate (booth #7326 ...
(Date:1/3/2017)... LAS VEGAS , Jan. 3, 2017 ... announced the introduction of Onitor Track, an innovative biometric ... and men, showcasing this month at the 2017 Consumer ... . In the U.S., the World ... affect more than two-thirds of adults who are overweight ...
Breaking Biology News(10 mins):