Navigation Links
Scientists use light to control proteins
Date:10/16/2008

A team of researchers from Penn State and the University of Texas Southwestern Medical Center has discovered a way to use light to control certain proteins that catalyze biochemical reactions. "This is one of the first examples of someone successfully controlling the activity of a protein using light," said Stephen Benkovic, Penn State Evan Pugh Professor of Chemistry, holder of the Eberly Family Chair in Chemistry, and one of the team's leaders. "The technology one day could be expanded to have multiple uses, including the ability to turn off the activities of some disease-causing proteins in the cell," he said. The team's results will appear in the 17 October issue of the journal Science.

In their experiment, the scientists designed a hybrid protein by inserting a light-sensing protein from an oat plant into an enzyme -- a type of protein that catalyzes biochemical reactions -- from the bacterium E. coli. After engineering the two components together, the researchers found that the enzyme's activity could be manipulated by shining a light on the light-sensing protein, which the scientists refer to as a "domain." "The technology works like a light switch," said Benkovic. "When we shine a light on the light-sensing domain, the enzyme's activity increases, and when we shut the light off, the enzyme's activity decreases."

According to Jeeyeon Lee, a postdoctoral scholar in the Penn State Department of Chemistry and one of the paper's authors, the team had to consider a number of factors when designing the hybrid protein, including the protein's shape, or what is referred to as its conformation. "The conformation of a protein is important in determining its function," she said. "Without the proper conformation, our protein would not have responded to the light."

Another important factor that the team had to consider was the proper location on the enzyme into which the light-sensing protein from the oat plant would be inserted. Vishal Nashine, also a postdoctoral scholar in the Department of Chemistry and an author of the paper, said the team was surprised to find that the switch worked only when they attached the light-sensing domain to the enzyme at a particular site. The switch did not operate when they attached the molecule to other locations on the enzyme. "The fact that the switch worked only when the light-sensing domain was attached to the enzyme at a specific site suggests that a unique network is active at that site through which signals, such as those responding to light, are transmitted," he said.

The successful site was located, among more than a hundred different possibilities, using a computational algorithm called the Statistical Coupling Analysis (SCA), which was pioneered by Rama Ranganathan, a professor of pharmacology at the University of Texas Southwestern Medical Center and one of the paper's leading authors.

The team's future research will investigate how the signal triggered by the light was transmitted from the light-sensing domain to the enzyme. "It is not yet clear how this process works," said Benkovic. "So far, the effect has been small, but we plan to optimize the technology to see if we can use light to modulate the enzyme's activity in alternative ways."


'/>"/>

Contact: Barbara K. Kennedy
science@psu.edu
814-863-4682
Penn State
Source:Eurekalert  

Related biology technology :

1. Nu Skin Scientists Correlate the Appearance of Aging With arNOX Free Radical Generator
2. Scientists engineer superconducting thin films
3. New nanoscale process created by UCSB scientists will help computers run faster and more efficiently
4. CU scientists create worlds thinnest balloon -- just one atom thick
5. Tengion Scientists Publish Positive Preclinical Findings With Neo-Organ Demonstrating Long-term Durability and Growth With Skeletal Maturation
6. Brewing better beer: Scientists determine the genomic origins of lager yeasts
7. Vermillion and Stanford Scientists Receive Best Research Award From the PAD Coalition
8. SACHEM Launches 2-D HPLC e-Learning Program : New e-Learning Program Teaches Scientists How to Better Analyze and Prove Product Purity Through Greater Sensitivity and Precision in Identification of Trace Components
9. Scientists peel away the mystery behind golds catalytic prowess
10. The National Cancer Institute Joins the Global Community of Scientists Now Using BIOMARKERcenter From Thomson Reuters
11. Scientists grow nanonets able to snare added energy transfer
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scientists use light to control proteins
(Date:10/11/2017)... ... October 11, 2017 , ... At its ... announced Dr. Suneel I. Sheikh, the co-founder, CEO and chief research scientist of ... been selected for membership in ARCS Alumni Hall of Fame . ASTER ...
(Date:10/11/2017)... Oct. 11, 2017  VMS BioMarketing, a leading provider of ... oncology Clinical Nurse Educator (CNE) network, which will launch this ... communication among health care professionals to enhance the patient care ... staff, and other health care professionals to help women who ... ...
(Date:10/10/2017)... ... October 10, 2017 , ... San Diego-based team building ... corporate rebranding initiative announced today. The bold new look is part of a ... company moves into a significant growth period. , It will also expand its service ...
(Date:10/10/2017)... SomaGenics announced the receipt of a Phase ... (Single Cell), expected to be the first commercially available ... from single cells using NGS methods. The NIH,s recent ... development of approaches to analyze the heterogeneity of cell ... for measuring levels of mRNAs in individual cells have ...
Breaking Biology Technology:
(Date:4/13/2017)... 13, 2017 UBM,s Advanced Design and Manufacturing ... feature emerging and evolving technology through its 3D Printing ... run alongside the expo portion of the event and ... demonstrations focused on trending topics within 3D printing and ... manufacturing event will take place June 13-15, 2017 at the ...
(Date:4/11/2017)... Florida , April 11, 2017 ... a security technology company, announces the appointment of independent Directors ... Bendheim to its Board of Directors, furthering the company,s ... ... of NXT-ID, we look forward to their guidance and benefiting ...
(Date:4/4/2017)...   EyeLock LLC , a leader of iris-based ... Patent and Trademark Office (USPTO) has issued U.S. Patent ... an iris image with a face image acquired in ... 45 th issued patent. "The ... the multi-modal biometric capabilities that have recently come to ...
Breaking Biology News(10 mins):