Navigation Links
Scientists target bacterial transfer of resistance genes
Date:10/25/2012

CHAMPAIGN, Ill. The bacterium Streptococcus pneumoniae which can cause pneumonia, meningitis, bacteremia and sepsis likes to share its antibiotic-defeating weaponry with its neighbors. Individual cells can pass resistance genes to one another through a process called horizontal gene transfer, or by "transformation," the uptake of DNA from the environment.

Now researchers report that they can interrupt the cascade of cellular events that allows S. pneumoniae to swap or suck up DNA. The new findings, reported in the journal PLoS ONE, advance the effort to develop a reliable method for shutting down the spread of drug resistance in bacteria.

"Within the last few decades, S. pneumoniae has developed resistance to several classes of antibiotics," said University of Illinois pathobiology professor Gee Lau, who led the study. "Importantly, it has been shown that antibiotic stress the use of antibiotics to treat an infection can actually induce the transfer of resistance genes among S. pneumoniae. Our approach inhibits resistance gene transfer in all strains of S. pneumoniae, and does so without increasing selective pressure and without increasing the likelihood that resistant strains will become dominant."

Lau and his colleagues focused on blocking a protein that, when it binds to a receptor in the bacterial cell membrane, spurs a series of events in the cell that makes the bacterium "competent" to receive new genetic material. The researchers hypothesized that interfering with this protein (called CSP) would hinder its ability to promote gene transfer.

In previous work published late last year in the journal PLoS Pathogens, Lau's team identified proteins that could be made in the lab that were structurally very similar to the CSP proteins. These artificial CSPs can dock with the membrane receptors, block the bacterial CSPs' access to the receptors and reduce bacterial competence, as well as reducing the infectious capacity of S. pneumoniae.

In the new study, the researchers fine-tuned the amino acid structure of more than a dozen artificial CSPs and tested how well they inhibited the S. pneumoniae CSPs. They also tested their ability (or, more desirably, their inability) to mimic the activity of CSPs in bacterial cells.

"The chemical properties of individual amino acids in a protein can greatly influence the protein's activity," Lau said.

The team identified several artificial CSPs that both inhibited the bacterial CSPs and reduced S. pneumoniae competence by more than 90 percent.

"This strategy will likely help us reduce the spread of antibiotic-resistance genes among S. pneumoniae and perhaps other species of streptococcus bacteria," Lau said.


'/>"/>
Contact: Diana Yates
diya@illinois.edu
217-333-5802
University of Illinois at Urbana-Champaign
Source:Eurekalert  

Related biology technology :

1. Scientists defuse the Vietnam time bomb
2. Singapore scientists lead human embryonic stem cell study
3. Sheffield scientists shine a light on the detection of bacterial infection
4. Nanowiggles: Scientists discover graphene nanomaterials with tunable functionality in electronics
5. Scientists solve mystery of colorful armchair nanotubes
6. iBioSeminars and iBioMagazine: Free, Online Biology Seminars and Short Talks by Leading Scientists
7. Chinese Scientists Zhen-Yi Wang and Zhu Chen Awarded 7th Annual Szent-Gyorgyi Prize for Progress in Cancer Research
8. Scientists decode brain waves to eavesdrop on what we hear
9. Receptos Scientists Publish Determination of a High Resolution Sphingosine 1-Phosphate Receptor 1 Structure in Science
10. Scientists learn how to out run damage with imaging technique
11. Design eye for the science guy: Drop-in clinic helps scientists communicate data
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Scientists target bacterial transfer of resistance genes
(Date:12/7/2016)... ... December 07, 2016 , ... JULABO USA is ... cart. The new website has been designed to provide the best user-friendly experience ... to access detailed product information, read educational industry content as well as share ...
(Date:12/6/2016)... , Dec. 6, 2016 /PRNewswire/ - SQI Diagnostics Inc. ("SQI" or ... results for the fourth quarter and fiscal year ended September 30, ... , , ... -based life sciences and diagnostics company that develops and commercializes ... Commercial Highlights Achieved revenues of $1.4 million more ...
(Date:12/6/2016)... ... December 06, 2016 , ... Superior Controls, Inc ... today announced the company has successfully completed its 50th consecutive audit. For ... systems integration services to leading companies in life sciences, food and beverage, energy ...
(Date:12/6/2016)... DIEGO , Dec. 6, 2016  Creative Medical ... Kesari , MD, PhD, FANA, FAAN to the Company,s ... neurology and clinical trials to assist the Company,s clinical ... stroke. The AmnioStem product is a universal donor stem cell ... activity in animal models of stroke 1 .  ...
Breaking Biology Technology:
(Date:11/24/2016)... 23, 2016 Cercacor today introduced Ember TM ... trainers non-invasively measure hemoglobin, Oxygen Content, Oxygen ... Respiration Rate in approximately 30 seconds. Smaller than a ... immediate access to key data about their bodies to ... regimen. Hemoglobin carries oxygen to muscles. ...
(Date:11/22/2016)... , Nov. 22, 2016   MedNet Solutions , ... entire spectrum of clinical research, is pleased to announce ... LiveWire Healthcare and Life Sciences Awards as "Most ... off an unprecedented year of recognition and growth for ... over 15 years. iMedNet ™ ...
(Date:11/19/2016)... 18, 2016 Securus Technologies, a leading provider ... safety, investigation, corrections and monitoring, announced today that it ... to have an independent technology judge determine who has ... high tech/sophisticated telephone calling platform, and the best customer ... do most of what we do – which clearly ...
Breaking Biology News(10 mins):