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Scientists take up golf to prove long-standing theory of cell stickiness
Date:10/4/2011

age due to toxins."

To fabricate the intricate details of the micro putter, charged particles were fired at a cantilever, using a technique known as focused ion beam (FIB), to remove material from its surface.

The micro putter was used to test the adhesion of live and dead yeast cells that were cultured in the lab for two days. The tests were carried out in an environmental scanning electron microscope (ESEM) a high-powered microscope that allows the user to image specimens when they are wet, meaning cells can be visualised in their natural environment.

Once inside the ESEM, the yeast cells were delicately placed on three surfaces of varying cell adhesion tungsten, gold and indium tin oxide and nudged with the micro putter until they were removed from their spot. The microscope was able to capture images of the movement of the cells whilst the tiny deflection of the putter was recorded as a measure of the cell's adhesion.

The adhesion force of a living cell was approximately 19 micronewtons, three times the force of a dead cell which had a value of approximately six micronewtons, confirming the 'cell adhesion model'.

"We hope the micro putter can be used to evaluate the actual health conditions of cells in the future. Combined with a single cell surgery technique, the micro putter could help to understand the mechanisms of disease and develop effective new drugs," continued Shen.


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Contact: Michael Bishop
michael.bishop@iop.org
01-179-301-032
Institute of Physics
Source:Eurekalert

Page: 1 2

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