Navigation Links
Scientists discover how cancer may take hold

Stanford, CA-- A team, led by researchers at the Carnegie Institution,* has found a key biochemical cycle that suppresses the immune response, thereby allowing cancer cells to multiply unabated. The research shows how the biomolecules responsible for healthy T-cells, the bodys first defenders against hostile invaders, are quashed, permitting the invading cancer to spread. The same cycle could also be involved in autoimmune diseases such as multiple sclerosis. The work is published in the September 25, 2007, issue of PLoS Biology.

The scientists used special molecular nanosensors for the work. We used a technique called fluorescence resonance energy transfer, or FRET, to monitor the levels of, tryptophan, one of the essential amino acids human cells need for viability, explained lead author Thijs Kaper. Humans get tryptophan from foods such as grains, legumes, fruits, and meat. Tryptophan is essential for normal growth and development in children and nitrogen balance in adults. T-cells also depend on it for their immune response after invading cells have been recognized. If they dont get enough tryptophan, the T-cells die and the invaders remain undetected.

The scientists looked at the chemical transformations that tryptophan undergoes as it is processed in live human cancer cells. When tryptophan is broken down in the cancer cells, an enzyme (dubbed IDO) forms molecules called kynurenines. This reduces the concentration of tryptophan in the local tissues and starves T-cells for tryptophan. A key finding of the research was that a transporter protein (LAT1), present in certain types of cancer cells, exchanges tryptophan from the outside of the cell with kynurenine inside the cell, resulting in an excess of kynurenine in the body fluids, which is toxic to T-cells.

Its double trouble for T-cells, remarked Wolf Frommer. Not only do they starve from lack of tryptophan in their surroundings, but it is replaced by the toxic kynurenines, which wipes T-cells out.

The scientists think that this cycle may be also be involved in cells involved in certain autoimmune diseases. In these cases the cells may not be able to take up or convert enough tryptophan. Without enough of the amino acid or the IDO enzyme to convert tryptophan, the cells cannot produce enough kynurenine. Lacking kynurenine, the bodys own T-cells cannot be kept in check, so they rebel and attack the body.

The FRET system detects metabolites such as sugars and amino acids using a biosensor tag. A protein is genetically fused to tags at opposite ends of a molecule. The tags are made from different colors of the jellyfish green fluorescent protein (GFP). When a metabolite binds to the biosensor, it changes the shape of the sensors backbone, altering the position of the fluorescent tags. When a specific wavelength of light activates one tag, it fluoresces. When the metabolite causes the tags to move close together, the other tag will also fluoresceresonating like a tuning fork. This system allows the scientists to visually track the location and concentration of certain biochemicals.

Our FRET technology with the novel tryptophan nanosensor has an added bonus, said Thijs. It can be used to identify new drugs that could reduce the ability of cancer cells to uptake tryptophan or their ability to degrade it. We believe that this technology could be a huge boost to cancer treatment.


Contact: Wolf Frommer
650-325-1521 x208
Carnegie Institution

Related biology technology :

1. UW computer scientists fighting computer virus "Cold War"
2. Scientists find way to make human collagen in lab
3. Wisconsin scientists to be recognized for innovative biofuel technology
4. UW-Madison scientists to mimic nature for newest cancer drugs
5. UW scientists study strange material with communications potential
6. Scientists find nanotech method for examining cells
7. UW space scientists use Keck telescope to study wild weather of Uranus
8. UW computer scientists tout achievements and explain industry shortcomings
9. Facing shortage of U.S. scientists, UW wants to boost math enrollment
10. UW-Madison scientists find a key to cell division
11. TIP/UW Scientists Provide Mars Rover Commentary
Post Your Comments:
(Date:11/24/2015)... SAN DIEGO , Nov. 24, 2015 Halozyme Therapeutics, ... Jaffray Healthcare Conference in New York on ... Dr. Helen Torley , president and CEO, will provide a ... New York at 1:00 p.m. ET/10:00 a.m. ... communication and investor relations, will provide a corporate overview. --> ...
(Date:11/24/2015)... Inc., a worldwide provider of clinical research services headquartered in ... has set a new quarterly earnings record in Q3 of 2015.  ... Q3 of 2014 to Q3 of 2015.   ... the establishment of an Asia-Pacific office to ... and Mexico , with the establishment ...
(Date:11/24/2015)... November 24, 2015 --> ... research report released by Transparency Market Research, the global ... a CAGR of 17.5% during the period between 2014 ... - Global Industry Analysis, Size, Volume, Share, Growth, Trends ... prenatal testing market to reach a valuation of US$2.38 ...
(Date:11/24/2015)... ... ... InSphero AG, the leading supplier of easy-to-use solutions for production, culture, and ... as Chief Operating Officer. , Having joined InSphero in November 2013 as ... promoted to Head of InSphero Diagnostics in 2014. There she has built up ...
Breaking Biology Technology:
(Date:11/9/2015)... , Nov. 9, 2015  Synaptics Inc. (NASDAQ: ... today announced broader entry into the automotive market with ... match the pace of consumer electronics human interface innovation. ... are ideal for the automotive industry and will be ... Europe , Japan ...
(Date:10/29/2015)... , Oct. 29, 2015  The J. Craig Venter ... titled, "DNA Synthesis and Biosecurity: Lessons Learned and Options ... of Health and Human Services guidance for synthetic biology ... --> --> ... has the potential to pose unique biosecurity threats. It ...
(Date:10/29/2015)... , October 29, 2015 ... authentication company focused on the growing mobile commerce ... announces that StackCommerce, a leading marketplace to discover ... the Wocket® smart wallet on StackSocial for this ... ("NXT-ID" or the "Company"), a biometric authentication company ...
Breaking Biology News(10 mins):