Navigation Links
Scientists Find Way to Fast-Track Production of Stem Cells that Show Promise in Treating AMD
Date:4/18/2013

Durham, NC (PRWEB) April 18, 2013

Controlling the differentiation of human pluripotent stem cells is the goal of many laboratories, both to study normal human development and to generate cells for transplantation in treating various diseases and conditions. RPE (retinal pigmented epithelial) is one important cell type under investigation as it protects and nourishes the photoreceptors and is vital in maintaining healthy eyesight.

In fact, the dysfunction and death of RPE is thought to be behind the leading cause of blindness in the Western world — age related macular degeneration.

Transplantation of RPE cells into the retina to treat AMD has been demonstrated in animals and is now being tested in clinical trials in humans. However, protocols to generate RPE from human pluripotent stem cells are time consuming and relatively inefficient. But a team of scientists at the University of California, Santa Barbara, reports in the latest issue of STEM CELLS Translational Medicine that it has found a way to isolate RPE cells as early as 14 days following the onset of differentiation.

"RPE cells are required for visual function and are a reasonable candidate for use in cellular therapy to treat macular degeneration. This study shows that it is now possible to produce homogeneous cultures in a shorter period of time," said James Thomson, a Wisconsin-based researcher who was the first to isolate human embryonic stem cells, commenting on the study.

The UCSB research team was led by Dennis Clegg, Ph.D., Peter Coffey, Ph.D., and David Buchholz, Ph.D. They based their study on earlier reports that neural retinal progenitors could be generated through the application of a handful of regulatory proteins, known as factors, that stimulate cell growth and function.

“As RPE and the neural retina arise from a common progenitor pool, we sought to determine whether this protocol could be altered to direct pluripotent stem cells to RPE with a similar efficiency,” Dr. Clegg explained. “Through the combined use of the retinal inducing factors IGF1, Noggin, Dkk1, bFGF and other factors added at specific times, we found that pluripotent stem cells could be directed to RPE, also with an efficiency of about 80 percent — and it only took 14 days. ”

“Protocols currently being used to generate RPE cells take months; this protocol should be useful for quickly generating quantities of RPE cells for transplantation as well as for the study of RPE development,” Dr. Buchholz added.

“This report is important because stem cell derived RPE are currently in clinical trials for the treatment of age related macular degeneration and Stargardt disease,” said Anthony Atala, M.D., Editor of STEM CELLS Translational Medicine and director of the Wake Forest Institute for Regenerative Medicine. “The protocol could prove useful for rapidly generating banks of cells for treating these conditions.”

###

The full article, “Rapid and efficient directed differentiation of human pluripotent stem cells into retinal pigmented epithelium,” can be accessed at http://www.stemcellstm.com.

About STEM CELLS Translational Medicine: STEM CELLS TRANSLATIONAL MEDICINE (SCTM), published by AlphaMed Press, is a monthly peer-reviewed publication dedicated to significantly advancing the clinical utilization of stem cell molecular and cellular biology. By bridging stem cell research and clinical trials, SCTM will help move applications of these critical investigations closer to accepted best practices.

About AlphaMed Press: Established in 1983, AlphaMed Press with offices in Durham, NC, San Francisco, CA, and Belfast, Northern Ireland, publishes two other internationally renowned peer-reviewed journals: STEM CELLS® (http://www.StemCells.com), in its 31st year, is the world's first journal devoted to this fast paced field of research. The Oncologist® (http://www.TheOncologist.com), also a monthly peer-reviewed publication, entering its 18th year, is devoted to community and hospital-based oncologists and physicians entrusted with cancer patient care. All three journals are premier periodicals with globally recognized editorial boards dedicated to advancing knowledge and education in their focused disciplines.

Read the full story at http://www.prweb.com/releases/2013/4/prweb10646350.htm.


'/>"/>
Source: PRWeb
Copyright©2012 Vocus, Inc.
All rights reserved

Related biology technology :

1. A bright idea: Tiny injectable LEDs help neuroscientists study the brain
2. Highly lethal Ebola virus has diagnostic Achilles heel for biothreat detection, scientists say
3. NineSigma Announces Winners in $25,000 Sustainable Packaging Challenge in Conjunction With Scientists Without Borders and Partners
4. Research Team led by JCVI Scientists Uncover More Complexity and Detail in Southern African Genomic Diversity
5. Dr. Ali S. Faqi and Leading Scientists Author Ground-Breaking Toxicology Book from Elsevier
6. Scientists discover molecule that does double duty in stopping asthma attacks
7. Scientists Warn of Long Term Consequences of Budget Sequestration: BioInformatics LLC Report
8. UC Santa Barbara scientists develop a whole new way of harvesting energy from the sun
9. The Elsevier Foundation, TWAS, and OWSD Honor Early Career Women Scientists in Developing Countries at the Annual AAAS Meeting
10. BioInformatics LLC New Market Report – Best Practices Guide for Advertising to Life Scientists
11. Science of Hiring: Demand for Scientists Grows 15%
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/11/2016)... ... , ... Global Stem Cells Group, a world ... Cells Network (GSCN) to distribute exosome injection and other biological products to stem ... Dominican Republic, Colombia, Argentina, Nicaragua, Panama, El Salvador, Venezuela, Peru, Ecuador, Paraguay, Puerto ...
(Date:2/10/2016)... Md. , Feb. 10, 2016  The Maryland ... Busch , has announced that University of Maryland School ... PhD, MBA and University of Maryland Medical System President ... of the "Speaker,s Medallion," the highest honor given to ... of Delegates. Dean Reece and Mr. ...
(Date:2/10/2016)... -- IsoRay, Inc. (NYSE MKT: ISR), a medical technology company ... for the treatment of prostate, brain, lung, head and ... for the second quarter and six months of fiscal ... --> --> Revenue was $1.19 ... ended December 31, 2015, a 12% increase compared to ...
(Date:2/10/2016)... ... 2016 , ... HOLLOWAY AMERICA, a leading custom stainless steel ... Chapter 21st Annual Vendor Exhibition on Thursday, February 18, 2016. The Rocky Mountain ... annual event, which will run from 3:00 p.m. - 8:30 p.m. at The ...
Breaking Biology Technology:
(Date:2/8/2016)... Republic , February 8, 2016 ... global payment platform which presents innovation for clients, ... Authentication feature called VoiceKey. --> Worldcore ... which presents innovation for clients, comfort and unbeatable ... VoiceKey. --> Worldcore is ...
(Date:2/4/2016)... The field of Human Microbiome research ... popular hubs of the biotechnology industry. While the ... of human microbiota, have garnered a lot of ... space has literally exploded in terms of both ... focuses on biomedical aspects of research, development, and ...
(Date:2/3/2016)... 3, 2016 --> ... report "Automated Fingerprint Identification System Market by Component (Hardware ... (Banking & Finance, Government, Healthcare, and Transportation) and Geography ... market is expected to be worth USD 8.49 Billion ... 2015 and 2020. The transformation and technology evolution from ...
Breaking Biology News(10 mins):