Navigation Links
Sangamo BioSciences Presents Data at ICAAC Demonstrating 'In Vivo' Protection Against HIV Infection by CCR5-ZFN Therapeutic
Date:10/28/2008

ic ZFNs resulted in the generation of an HIV-resistant population of primary human T-cells by the permanent genetic modification of the CCR5 gene. These ZFN-modified CD4 T-cells expanded stably in HIV-infected cultures for several weeks and appeared to behave identically to untreated T-cells except that they were resistant to infection by HIV. ZFN treated primary CD4 T-cells and transformed CD4 cell lines resisted infection with R5-tropic HIV (virus that uses the CCR5 co-receptor to enter cells), resulting in enrichment of ZFN-generated CCR5-disrupted cells in the population upon exposure to virus. Importantly, in the presence of HIV, ZFN-modified CD4 T-cells also preferentially expanded in a mouse model. The modified cells were infused into mice that lack a normal immune system and thus do not reject human cells. After 33 days, the mice were sacrificed and analyzed for the presence of ZFN-modified cells. Researchers determined that ZFN-modified cells engrafted normally in the mouse and that the proportion of modified cells present at the end of the experiment was greater than two to three fold higher in mice in the presence of HIV infection (p=0.008). It was also determined that 50 days after infection, mice given the ZFN-modified cells had increased numbers of CD4 cells and a statistically significant seven-fold reduction in viral load in their peripheral blood (P<0.001) compared to mice given control cells. A high level of specificity of the CCR5-ZFNs for their target site was demonstrated by immunochemistry and direct genomic sequence analysis of ZFN-treated human CD4 T-cells. These data suggest that, in the presence of HIV, the ZFN-modified cells have a selective advantage allowing them to evade infection and destruction leaving them able fight opportunistic infections and HIV itself.

In addition, Sangamo and its collaborators have demonstrated successful ZFN-modification of clinical-scale quantities of human CD4 T-cells and that these modified cells exh
'/>"/>

SOURCE Sangamo BioSciences, Inc
Copyright©2008 PR Newswire.
All rights reserved

Page: 1 2 3 4 5

Related biology technology :

1. Sangamo BioSciences Announces Third Quarter Conference Call and Webcast
2. Sangamo BioSciences to Present at Two Upcoming Investor Conferences
3. Sangamo BioSciences to Present at the UBS Global Life Sciences Conference
4. Sangamo BioSciences to Present at the Canaccord Adams Global Growth Conference
5. Sangamo BioSciences to Present at the BMO Capital Markets Focus on Healthcare Conference
6. Sangamo BioSciences Reports Second Quarter 2008 Financial Results
7. Sangamo BioSciences Announces Second Quarter Conference Call and Webcast
8. Sangamo BioSciences and Sigma-Aldrich Announce Research and License Agreement With Roche for the Use of ZFN Technology for Generation of Transgenic Animals
9. Dow AgroSciences Announces Early Exercise of Option for Commercial License With Sangamo BioSciences for Plants
10. Dow AgroSciences, Sangamo BioSciences Announce the Achievement of Key Milestones in Plant Agriculture Collaboration
11. Sangamo BioSciences to Present at the Canaccord Adams Diabetes and Obesity Conference
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/29/2014)... A major challenge before new biotechnology start-up ... of Boston-Cambridge, is gaining visibility that can lead to ... Director of Boston’s Adult Stem Cell Technology Center, LLC ... of his company an important priority since he started ... a social media marketing campaign launched earlier ...
(Date:8/28/2014)... USA (PRWEB) August 28, 2014 SPIE ... of Physics and Optical Science and Director of the ... of North Carolina at Charlotte, and co-founder and Chairman ... to serve as the 2015 Vice President of SPIE, ... 2014 President Philip Stahl announced recent election results at ...
(Date:8/28/2014)... 28, 2014 Best Sanitizers, Inc., the ... alcohol-based hand sanitizer, asks food processors and food handling ... hand sanitizer they’re currently using to Best Sanitizers’ ... is critical to fighting cross-contamination and the spread of ... believes there are key criteria that make a hand ...
(Date:8/28/2014)... A new research platform uses a laser ... structures undergoing stress and heating, an approach ... for microelectronics and batteries., This new technique, ... how heating and the surface stress of ... have discussed the merits of surface-stress influence ...
Breaking Biology Technology:The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 3The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 4Glenn Boreman, UNC Professor and Plasmonics Co-Founder, Elected to SPIE Presidential Chain 2Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 2Food Processors and Food Handlers are Encouraged to Prepare for Fall Harvest by Evaluating Their Current Hand Sanitizer Using Nine Key Criteria 3New analytical technology reveals 'nanomechanical' surface traits 2New analytical technology reveals 'nanomechanical' surface traits 3
... ... line of Spectrum benchtop laboratory equipment that is now available for the budget-conscious researcher ... baths, mini centrifuges, hotplates & magnetic stirrers for general sample preparation and laboratory mixing ... Gardena, ...
... ... general release , ... Charlottesville, VA, San Francisco, CA, New York, NY and Paris, France (PRWEB) January 8, ... software solution for managing and reporting aggregate spend. The company will be officially launching ...
... , , HAYWARD, Calif., Jan. 8 ... that it has obtained commitments from accredited institutional investors ... and warrants in a private placement. The financing was ... Great Point Partners along with participation from other existing ...
Cached Biology Technology:Spectrum Introduces New Line of Spectrum Branded Benchtop Laboratory Equipment 2OpenQ Announces openSpend for Physician Payment Sunshine Act Compliance 2Solta Medical to Raise $17.2 Million in Private Placement 2Solta Medical to Raise $17.2 Million in Private Placement 3Solta Medical to Raise $17.2 Million in Private Placement 4Solta Medical to Raise $17.2 Million in Private Placement 5
(Date:8/28/2014)... Research Careers) Program has announced the travel award recipients ... 18 22, 2014 in San Diego, California. ... students, post doctorates and scientists from underrepresented groups into ... encourage the participation of young scientists at the American ... 16 awards totaling $29,600., The FASEB MARC Program is ...
(Date:8/28/2014)... developmental on-off switch for Streptomyces , a group ... the world,s naturally derived antibiotic medicines. , Their ... possible to manipulate this switch to make nature,s antibiotic ... 28 in Cell , found that a unique ... larger protein called BldD ultimately controls whether a bacterium ...
(Date:8/28/2014)... method for measuring and imaging how quickly blood flows ... understand how drug abuse affects the brain, which may ... lead to better treatment options for recovering drug addicts. ... from Stony Brook University in New York, USA and ... in The Optical Society,s (OSA) open-access journal ...
Breaking Biology News(10 mins):Small molecule acts as on-off switch for nature's antibiotic factory 2Small molecule acts as on-off switch for nature's antibiotic factory 3This is your brain's blood vessels on drugs 2This is your brain's blood vessels on drugs 3
... flying in chevron formation or cyclists learned the value of ... their spores so as to spread them as high and ... Sclerotinia sclerotiorum , spews thousands of spores nearly simultaneously to ... even creates a wind that carries many of the spores ...
... to clean water and environmental causes, his dedication as ... the pursuit of discovery, Professor Xiaoguang Meng received an ... Technology at the university,s annual Convocation Ceremony on September ... Charles V. Schaefer, Jr. School of Engineering and Science, ...
... is now open to the press for the ... of America (ESA) and National Education Association, with ... October 14-15, 2010 in Washington, D.C. The summit ... educators, scientists, and policy professionals from a wide ...
Cached Biology News:Fungal spores travel farther by surfing their own wind 2Fungal spores travel farther by surfing their own wind 3Fungal spores travel farther by surfing their own wind 4Prof. Xiaoguang Meng receives honorary master of engineering from Stevens 2Prof. Xiaoguang Meng receives honorary master of engineering from Stevens 3Ecology and Education Summit to address environmental literacy 2
... units/µL ,• High specific activity and processivity ... 'A' overhang ,• Processes ... high activity in primer extension and other ... of the enzyme, each lot has been ...
Recombinant DNA polymerase from Thermus aquaticus expressed in E. coli; 5 units/L; 1 tube of 1, 000 units enzyme/tube w/Buffer II and MgCl2...
... 5 units/µL ,• High specific activity and ... an 'A' overhang ,• Processes ... high activity in primer extension and other ... of the enzyme, each lot has been ...
Recombinant DNA polymerase from Thermus aquaticus expressed in E. coli; 5 units/uL; 25 tubes of 1, 000 units enzyme/tube w/ Buffer I...
Biology Products: