(PRWEB) September 30, 2013
German scientists were seeking to compare studies that used different assays. In their study, published in the January 2013 issue of Psychoneuroendocrinology, they concluded that among commercially available assays, the Salimetrics assay for cortisol exhibits the best linearity and comes closest to the results found in tandem mass spectrometry. They confirmed that the different cortisol assays of Salimetrics, IBL, DRG, DSL and DELFIA produced results which could not be easily compared.
The scientists further suggest the inclusion of nonlinear model components to compensate for the “accuracy decrease towards lower cortisol values” which “is assumed to reflect the impact of cross-reactive saliva matrix antigens, mostly cortisone.”
“Of all the commercially available immunoassays, Salimetrics has the least cross reactivity with cortisone and therefore the Salimetrics product is the most accurate,” Salimetrics Principal Scientist Jon Peterson, Ph.D., explained. He added that “it is the mission of Salimetrics to provide immunoassays that are the standard for saliva-based biomarker research.”
The vast majority of salivary cortisol measurements are determined using immunoassays, the study’s authors, Robert Miller, Franziska Plessow, Manfred Rauh, Michael Groschl and Clemens Kirschbaum, noted. “Due to its non-invasiveness, precision and cost efficiency, the measurement of steroid levels from saliva has become a valuable tool in psychoneuroendocrinological research over the past two decades.” The scientists created models to compare the accuracies of different assay products to liquid chromatography coupled with tandem mass spectroscopy (LC-MS/MS) control results. These models showed that the Salimetrics assay
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