Navigation Links
Researchers use X-ray diffraction microscope to reveal 3-D internal structure of whole cell
Date:6/15/2010

Three-dimensional imaging is dramatically expanding the ability of researchers to examine biological specimens, enabling a peek into their internal structures. And recent advances in X-ray diffraction methods have helped extend the limit of this approach.

While significant progress has been made in optical microscopy to break the diffraction barrier, such techniques rely on fluorescent labeling technologies, which prohibit the quantitative 3-D imaging of the entire contents of cells. Cryo-electron microscopy can image structures at a resolution of 3 to 5 nanometers, but this only works with thin or sectioned specimens.

And although X-ray protein crystallography is currently the primary method used for determining the 3-D structure of protein molecules, many biological specimens such as whole cells, cellular organelles, some viruses and many important protein molecules are difficult or impossible to crystallize, making their structures inaccessible. Overcoming these limitations requires the employment of different techniques.

Now, in a paper published May 31 in Proceedings of National Academy of Sciences, UCLA researchers and their collaborators demonstrate the use of a unique X-ray diffraction microscope that enabled them to reveal the internal structure of yeast spores. The team reports the quantitative 3-D imaging of a whole, unstained cell at a resolution of 50 to 60 nanometers using X-ray diffraction microscopy, also known as lensless imaging.

Researchers identified the 3-D morphology and structure of cellular organelles, including the cell wall, vacuole, endoplasmic reticulum, mitrochondria, granules and nucleolus. The work may open a door to identifying the individual protein molecules inside whole cells using labeling technologies.

The lead authors on the paper are Huaidong Jiang, a UCLA assistant researcher in physics and astronomy, and John Miao, a UCLA professor of physics and astrono
'/>"/>

Contact: Jennifer Marcus
jmarcus@cnsi.ucla.edu
310-267-4839
University of California - Los Angeles
Source:Eurekalert

Page: 1 2 3

Related biology technology :

1. Researchers discover new properties of Worlds thinnest material
2. Sandia researchers Phillips, Moody named MRS Fellows
3. First Self-Replicating, Synthetic Bacterial Cell Constructed by J. Craig Venter Institute Researchers
4. Investigating how spiders spin their silk, researchers unravel a key step
5. UCLA researchers use new microscope to see atoms for first time
6. NIST researchers holding steady in an atomic-scale tug-of-war
7. Researchers use improved nanogenerators to power sensors based on zinc oxide nanowires
8. Researchers identify a gene that may play a key role in atherosclerosis and other diseases
9. Thomson Reuters Names the Worlds Hottest Researchers
10. Cleveland researchers use natural and artificial sheaths to mend traumatic bone loss
11. MIT researchers discover new way of producing electricity
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/10/2014)... researchers at the University of Illinois at Urbana-Champaign ... the interplay of spin and heat at the ... spintronic devices for data storage and information processing. ... as spin-angular momentum. In a typical charge current, ... spin current," explained David Cahill, a professor of ...
(Date:1/15/2014)... approach akin to assembling a club sandwich at ... (NIST) researchers have succeeded in crafting a uniform, ... of foam commonly used in upholstered furniture and ... nanotube-coated polyurethane foam was reduced 35 percent compared ...
(Date:1/15/2014)... JOLIET, Ill. , Jan. 15, 2014  Bill Jacobs Automotive, ... , is teaming up with the Heartland Blood Center ... annual blood drive. The drive takes place Saturday, Jan. 18 ... a.m. - 2 p.m. (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  ...
(Date:1/15/2014)... FALLS, N.Y., Jan. 15, 2014   Niagara Thermal ... been selected by Buffalo BioBlower Technologies LLC ("B3") as ... SBIR Phase 2 award from the Army Corps of ... system that kills all biological contaminants and destroys VOCs ...
Breaking Biology Technology:University of Illinois study advances limits for ultrafast nano-devices 2Layered security: Carbon nanotubes promise improved flame-resistant coating 2Join the Bill Jacobs Auto blood drive and receive a free oil change 2Niagara Thermal Products LLC Selected as Critical Supplier for Buffalo BioBlower Air Purification Systems 2
... can make a professor lose his cool more than having ... for making a mistake are endless: a DVD made specifically ... skipping around uncontrollably or even an overhead projector falling to ... duty of solving such technical issues and more falls on ...
... Madison, Wis. - If wireless keeps growing at its ... get their work done, says a wireless channel manager for ... at a recent Accelerate Madison program, saying the ... for corporations over the next few years. With costs falling ...
... Eau Claire, Wis . Faculty in the chemistry ... more than $600,000 in grant money from the National ... help them purchase state-of-the-art instrumentation and support faculty-student research. ... a department like ours and it'll have a significant ...
Cached Biology Technology:Interest drives investment of instructional technology 2Interest drives investment of instructional technology 3Interest drives investment of instructional technology 4Cisco manager touts wireless advantages 2UW-Eau Claire chemistry faculty get $600,000 in NSF grants 2UW-Eau Claire chemistry faculty get $600,000 in NSF grants 3
(Date:7/10/2014)... NEW YORK , June 27, 2014 /PRNewswire/ ... against Provectus Biopharmaceuticals, Inc. ("Provectus" or the "Company")(NYSE-MKT: ... action, filed in United States District Court, Middle ... Division, is on behalf of a class consisting ... otherwise acquired Provectus securities between December 17, 2013 ...
(Date:7/10/2014)... -- NXT-ID, Inc., (OTCQB: NXTD) a biometric authentication company focused on the ... was interviewed on July 1 st on the ... . Mr. Pereira discusses the company,s next generation smart wallet ... the Wocket™ aims to replace a traditional wallet with a ... tells Gino he has never heard of the Wocket ...
(Date:7/10/2014)... GOTHENBURG, Sweden , July 8, 2014 ... FPC1021 has won a new design win (DW). An Asian ... targeted date for mass production start in August 2014.   ... an Asian smartphone OEM, which has a planned date for ... received an initial ramp order of SEK 5M, for delivery ...
Breaking Biology News(10 mins):SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 2Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 4FPC Wins DW and Initial Ramp Order for FPC1021 Touch Sensor for Flagship Smartphone from Asian OEM 2
... biology textbooks, a single genetic mutation is rarely enough to ... a series of mutations that work together to trigger tumor ... that distinct cancer-causing mutations in neighboring cells can cooperate to ... Cancer biologists have long known that it takes ...
... , CARDIFF, Calif. , Jan. 13 ... Mind, Inc. was in high demand at this year,s Consumer Electronics Show ... many interviews with major media organizations during the 3 day event. Among ... Up TV , with Mark Albertson . Additionally, we have been ...
... Cold Spring Harbor, N.Y. Two scientists from ... international team that has discovered a genetic mutation that ... is a devastating inherited neurological disease that is very ... often among Ashkenazi Jews. The study was ...
Cached Biology News:Mutations in different cells cooperate to set the stage for cancer 2Mutations in different cells cooperate to set the stage for cancer 3Jedi Mind Receives Vast Media Coverage at CES in Las Vegas 2Jedi Mind Receives Vast Media Coverage at CES in Las Vegas 3CSHL scientists use next-gen sequencing to rapidly discover genetic cause of devastating disorder 2
This antibody is specific for human J-chain as defined by ELISA assay using purified human immunoglobulin fragments as solid phase target antigen....
Tissue-Stat ( Tissue Adherent for Mounting Formalin-Fixed and Frozen Tissue Sections.)...
Clarin Mounting Medium (Permanent environmentally safe mounting medium for general histology & cytology use)...
IHC Zinc Fixative - , 1000 ml Consult technical datasheet for details....
Biology Products: