Navigation Links
Researchers outline structure of largest nonvirus particle ever crystallized
Date:11/26/2007

Researchers at UCLA's California NanoSystems Institute, the David Geffen School of Medicine at UCLA and the Howard Hughes Medical Institute have modeled the structure of the largest cellular particle ever crystallized, suggesting ways to engineer the particles for drug delivery.

The research study, which focuses on new engineered nanomaterial vaults for use as a drug-therapy vehicle, appears in the Nov. 27 edition of the peer-reviewed, open-access journal PLoS Biology, published by the Public Library of Science, and is available at http://biology.plosjournals.org.

The team of researchers at UCLA is led by David Eisenberg and Leonard H. Rome of the departments of biological chemistry at the Geffen School of Medicine and the California NanoSystems Institute and associate researchers Daniel H. Anderson, Valerie A. Kickhoefer and Stuart A. Sievers. Eisenberg, Anderson and Sievers are also members of the Howard Hughes Medical Institute and the UCLAU.S. Department of Energy Institute for Genomics and Proteomics.

Vaults are large, barrel-shaped particles found in the cytoplasm of all mammalian cells; they may function in innate immunity. As naturally occurring nanoscale capsules, vaults may be useful to engineer as therapeutic delivery vehicles. For the study, the team of researchers proposed an atomic structure for the thin outer shell of the vault.

Using X-ray diffraction and computer modeling, the research team developed a draft atomic model for the major vault protein, which forms the shell-like enclosure of the vault.

"Our draft model is essentially an atomic-level vault with a completely unique structure, like a barrel with staves. It is unlike any other large structure found in nature," Rome said. "The outside of the vault structure is like an eggshell a continuous protective barrier with no gaps."

The shell is made up of 96 identical protein chains each made of 873 amino acid residues folded into 14 domains. Each chain forms an elongated stave of half the vault, as well as the cap of the barrel-like shell.

"These nanostructured vaults offer a human-friendly nanocontainer, like a molecular-level C-5A transport jet, with a cargo hold large enough to encompass a whole ribosome with its hundreds of proteins and nucleic acids, or enough drugs to control a cell," Eisenberg said.

The construction of the draft atomic model lays the foundation for further studies of vaults and will guide vault engineering projects focused on the targeted release of vault contents for drug delivery.


'/>"/>

Contact: Jennifer Marcus
jmarcus@cnsi.ucla.edu
310-267-4839
University of California - Los Angeles  
Source:Eurekalert

Related biology technology :

1. Researchers improve ability to write and store information on electronic devices
2. Long-awaited international ethical guidelines for biobank researchers
3. CU researchers shed light on light-emitting nanodevice
4. Stevens researchers provide new information about mass spectrometry
5. Researchers measure carbon nanotube interaction
6. Researchers underscore limitations of genetic ancestry tests
7. ASU researchers improve memory devices using nanotech
8. UD researchers race ahead with latest spintronics achievement
9. Anesiva Outlines Comprehensive Commercialization Plan for FDA Approved Product Zingo(TM)
10. New Initiative Has Shocking Effect: Thousands of Missourians with Abnormal Chromosome Structures Are Not Human
11. Two nanostructures are better than one
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researchers outline structure of largest nonvirus particle ever crystallized
(Date:8/18/2017)... ... August 18, 2017 , ... Producers of the award ... Inc. in an upcoming episode, scheduled to broadcast fourth quarter 2017. American Farmer ... Hybrids, the independent, family-owned seed company. Educating audiences about its broad portfolio of ...
(Date:8/16/2017)... PA (PRWEB) , ... August 16, 2017 , ... ... taking part in sessions at the ISPE Annual Meeting and Expo , to ... San Diego Marina. The event’s theme is “Driving innovation to advance patient therapies.” , ...
(Date:8/16/2017)... ... August 16, 2017 , ... Today, 3Bar ... has secured $2M in funding from an impressive group of investors, including Rev1 ... Thrive Fund. With this investment, 3Bar is broadening availability of its groundbreaking offering ...
(Date:8/15/2017)... After spending the past two years building a state-of-the-art technology which ... offers this platform to healthcare stakeholders (hospitals, foundations, biopharma companies etc.) ... collection vis a vis their members, under their own brand. Three ... offer. ... ...
Breaking Biology Technology:
(Date:5/6/2017)... 5, 2017 RAM Group , ... new breakthrough in biometric authentication based on a ... properties to perform biometric authentication. These new sensors are ... created by Ram Group and its partners. This sensor ... supply chains and security. Ram Group is a ...
(Date:4/19/2017)... 2017 The global military biometrics ... marked by the presence of several large global players. ... five major players - 3M Cogent, NEC Corporation, M2SYS ... nearly 61% of the global military biometric market in ... global military biometrics market boast global presence, which has ...
(Date:4/17/2017)... Florida , April 17, 2017 NXT-ID, ... technology company, announces the filing of its 2016 Annual Report on ... and Exchange Commission. ... on Form 10-K is available in the Investor Relations section of ... as on the SEC,s website at http://www.sec.gov . ...
Breaking Biology News(10 mins):