Researchers at UCLA's California NanoSystems Institute, the David Geffen School of Medicine at UCLA and the Howard Hughes Medical Institute have modeled the structure of the largest cellular particle ever crystallized, suggesting ways to engineer the particles for drug delivery.
The research study, which focuses on new engineered nanomaterial vaults for use as a drug-therapy vehicle, appears in the Nov. 27 edition of the peer-reviewed, open-access journal PLoS Biology, published by the Public Library of Science, and is available at http://biology.plosjournals.org.
The team of researchers at UCLA is led by David Eisenberg and Leonard H. Rome of the departments of biological chemistry at the Geffen School of Medicine and the California NanoSystems Institute and associate researchers Daniel H. Anderson, Valerie A. Kickhoefer and Stuart A. Sievers. Eisenberg, Anderson and Sievers are also members of the Howard Hughes Medical Institute and the UCLAU.S. Department of Energy Institute for Genomics and Proteomics.
Vaults are large, barrel-shaped particles found in the cytoplasm of all mammalian cells; they may function in innate immunity. As naturally occurring nanoscale capsules, vaults may be useful to engineer as therapeutic delivery vehicles. For the study, the team of researchers proposed an atomic structure for the thin outer shell of the vault.
Using X-ray diffraction and computer modeling, the research team developed a draft atomic model for the major vault protein, which forms the shell-like enclosure of the vault.
"Our draft model is essentially an atomic-level vault with a completely unique structure, like a barrel with staves. It is unlike any other large structure found in nature," Rome said. "The outside of the vault structure is like an eggshell a continuous protective barrier with no gaps."
The shell is made up of 96 identical p
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