Navigation Links
Researchers develop new way to see single RNA molecules inside living cells
Date:4/6/2009

Biomedical engineers have developed a new type of probe that allows them to visualize single ribonucleic acid (RNA) molecules within live cells more easily than existing methods. The tool will help scientists learn more about how RNA operates within living cells.

Techniques scientists currently use to image these transporters of genetic information within cells have several drawbacks, including the need for synthetic RNA or a large number of fluorescent molecules. The fluorescent probes developed at the Georgia Institute of Technology circumvent these issues.

"The probes we designed shine bright, are small and easy to assemble, bind rapidly to their targets, and can be imaged for hours. These characteristics make them a great choice for studying the movement and location of RNA inside a single cell and the interaction between RNA and binding proteins," said Philip Santangelo, an assistant professor in the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University.

Details of the probe production process and RNA imaging strategy were published online in the journal Nature Methods on April 6. In addition to Santangelo, Georgia Tech graduate student Aaron Lifland, Emory University associate professor Gary Bassell and Vanderbilt University professor James Crowe Jr. also contributed to this research. This research was funded by new faculty support from Georgia Tech.

In the study, the probes produced by attaching a few small fluorescent molecules called fluorophores to a modified nucleic acid sequence and combining the sequences with a protein exhibited single-molecule sensitivity and allowed the researchers to target and follow native RNA and non-engineered viral RNA in living cells.

"The great thing about these probes is that they recognize RNA sequences and bind to them using the same base pairing most people are familiar with in regards to DNA," explained Santangelo. "By addin
'/>"/>

Contact: Abby Vogel
avogel@gatech.edu
404-385-3364
Georgia Institute of Technology Research News
Source:Eurekalert  

Page: 1 2 3

Related biology technology :

1. Researchers peer into nanowires to measure dopant properties
2. Researchers find better way to manufacture fast computer chips
3. Collexis Connects Biomedical Researchers and Clinical Scientists with the Collexis Expert Platform for Translational Research
4. Researchers create catalysts for use in hydrogen storage materials
5. Thomson Reuters Names Hottest Researchers and Papers of 2007-2008
6. Researchers discover a potential on-off switch for nanoelectronics
7. University of Alberta and NINT researchers make solar energy breakthrough
8. Seminar Will Bring ALS Researchers to Patient Groups
9. Researchers at the University of Georgia Discover Protein is Crucial to Reproduction of Parasites Involved in Disease
10. FDA Approves Clinical Studies of a Novel Anti-Cancer Drug Developed by Italian Researchers
11. In Analyst Interview, Cord Blood America Says Stem Cells Now Front and Center for Researchers and Investors
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Researchers develop new way to see single RNA molecules inside living cells
(Date:8/28/2015)... Aug. 28, 2015 /PRNewswire/ - RepliCel Life Sciences ... medicine company focused on the development of autologous ... quarter ended June 30, 2015, corporate highlights, and ... report are available at www.sedar.com ,  ... "RepliCel expects to launch its RCS-01 ...
(Date:8/28/2015)... ... August 28, 2015 , ... Since the initial launch of FireflySci Inc. (FFS) in ... and excellent customer service. Images such as the Fonz in a lab coat and ... flavor to the humdrum spectroscopy field. , FireflySci is proud to be the ...
(Date:8/27/2015)... ... August 27, 2015 , ... Capital Edge Consulting ... business problems, is pleased to announce their ranking as one of the top 5000 ... the fastest-growing private companies across the nation. , “It is truly an honor to ...
(Date:8/27/2015)... , Aug. 27, 2015 /PRNewswire/ - Portage ... Exchange: PBT.U), and Biohaven Pharmaceutical Holding Company Limited ... a Phase I study of BHV-0223, a glutamate ... (IND) regarding BHV-0223 and recently obtained clearance from ... proceed with human testing. Portage holds 54% equity ...
Breaking Biology Technology:RepliCel Reports Second Quarter 2015 Financial Results 2RepliCel Reports Second Quarter 2015 Financial Results 3RepliCel Reports Second Quarter 2015 Financial Results 4RepliCel Reports Second Quarter 2015 Financial Results 5Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 2Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 3Portage's Biohaven doses first human subject in Phase 1 trial with lead drug candidate BHV-0223 4
... You Land a Job , ... San Mateo, CA (PRWEB) July 1, ... hesitate to use the Delete key on your professional resume or bio. ... also be a handicap in the job hunt, says a leading ...
... receive recombinant Bovine Somatotropin (rbST) make more milk, all the ... according to a Cornell University study to be published in ... (June 30, 2008.) , Producing milk uses large quantities ... biotech product used on American farms has been in ...
... Life,Sciences, Inc. (Nasdaq: CALP ), a leading provider ... research, today announced that it has been,added to the ... indexes captures the 4,000,largest U.S. stocks as of the ... in the Russell 3000, which remains in place for,one ...
Cached Biology Technology:Facelift for Resumes Offers Hope for Job Seekers, says Resume Writing Service 2Facelift for Resumes Offers Hope for Job Seekers, says Resume Writing Service 3Carbon hoofprint: Cows supplemented with rbST reduce agriculture's environmental impact 2Caliper Life Sciences Added to Russell 3000(R) Index 2
(Date:8/28/2015)... -- According to a new market research ... (Pen & Paper Based, Hosted, Biometrics), Service, Application (Clinical ... Vertical and Region - Global Forecast to 2020", published ... from USD 2.4 Billion in 2015 to USD 7.5 ... (CAGR) of 25.6% from 2015 to 2020. ...
(Date:8/26/2015)... The report "Multi-Factor Authentication (MFA) Market ... (Travel & Immigration, Government, Banking, Defense, Commercial Security, Consumer ... published by MarketsandMarkets, Multi-Factor Authentication Market is expected to ... CAGR of 17.7% between 2015 and 2020. ... spread through 169 P ages and ...
(Date:8/25/2015)... BELLEVUE, Wash. , Aug. 25, 2015 /PRNewswire/ ... announced today it will unveil its "Guardian S" snake ... the National Tactical Officers Association Conference on August 30 ... S is the first-ever commercially available energetically autonomous snake ... and in-field trials and is protected by more than ...
Breaking Biology News(10 mins):Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 2Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 3Cognitive Assessment and Training Market Worth 7.5 Billion USD by 2020 4Multi-Factor Authentication Market Worth 9.60 Billion USD by 2020 2Multi-Factor Authentication Market Worth 9.60 Billion USD by 2020 3Multi-Factor Authentication Market Worth 9.60 Billion USD by 2020 4Multi-Factor Authentication Market Worth 9.60 Billion USD by 2020 5Sarcos to Debut First-of-its-Kind Snake Robot 2Sarcos to Debut First-of-its-Kind Snake Robot 3
... Biologists have tracked down genes that control the handedness of ... the genes used by humans to set up the left ... online in advance of publication in Nature by ... have been responsible for establishing the left-right asymmetry of animals ...
... a licensing deal to enable bio-based production of ... drugs and solvents and as a food additive. ... advanced starch and starch-derivatives businesses, was intrigued by ... produce bio-based succinic acid from renewable resources via ...
... 2008 The Translational Genomics Research Institute (TGen), Scottsdale ... that could help cancer patients by stimulating the immune ... being conducted at TGen Clinical Research Services at Scottsdale ... Healthcare Corp., and at Mayo Clinic in Arizona. ...
Cached Biology News:Snails and humans use same genes to tell right from left 2Snails and humans use same genes to tell right from left 3Snails and humans use same genes to tell right from left 4'Green' chemicals closer to market 2TGen, Scottsdale Healthcare, Mayo Clinic study new drug to stimulate immune system of cancer victims 2
This kit is designed to select cells labeled with biotinylated antibodies. Kit includes reagents for labeling 3 x 10 9 cells and EasySep magnet....
Designed for use with the Agencourt CleanSEQ and Agencourt AMPure 384-well SPRI reagent kits. For use with 384-well thermal cycling plates....
SDS-OutT Precipitation Reagent...
Physical Form: saline suspension with ethanol Particle Dim: mean particle size 50 μm Matrix: HyperD F. Useful for the removal of detergents from protein samples....
Biology Products: