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Reportlinker Adds Sample Preparation and Library Preparation for DNA Sequencers (Results of The Kalorama Survey of Labs)
Date:1/11/2011

g Process
  • Sources of the Samples (human, bacteria, plant)

  • Types of Targeted Enrichment Products Used

  • Systems Used for Checking Concentration, Size, and/or Quality

  • Importance of Cost, Throughput, Flexibility, Reproducibility, Robustness, User-Friendliness, Accuracy and other factors

  • Brands Used for DNA or RNA Extraction and Purification

  • Brands Used for Fragmentation and Library Preparation

  • Brands Used for Amplification/ Polymerases, Dyes, and Sequencing Cleanup

  • Third Party Liquid Handlers Used

  • Barriers to 2nd- and 3rd-generation Sequencer Use

  • Analyst Findings

The study focuses on a survey of 120 laboratories which was carried out from July to September of 2010, with the majority in the latter portion. Along with questions about the specific sequencers used in the labs, the respondents were asked about the sample preparation and library preparation kits, reagents, and other consumables used for sequencing.

The survey effort targeted any lab that uses sequencing for any application, but with the goal of achieving as close as possible a representative breadth of labs. Due to the increasing importance of 2nd- and 3rd-generation systems in the market, the scope leans towards these types of sequencers.

However, the market inevitably also includes capillary sequencers, as 47 of the 87 surveyed labs with next-generation systems also have capillary sequencers. Around a third of the systems in the respondents' labs ove
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SOURCE Reportlinker
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