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ctrometry . 49

Imaging mass spectrometry .. 49

MALDI mass spectrometry for biomarker discovery 50

Quantitative tandem MS . 51 - 4 -

Single-molecule mass spectrometry using a nanopore .. 51

Requirements for MS-based proteomic biomarker development . 52

Protein tomography . 52

Protein biochips/microarrays and biomarkers . 52

Antibody-based biomarker discovery .. 53

Detection of biomarkers using peptide array technology .. 53

Protein nanobiochip .. 53

Gene expression microarray data as a source of protein biomarkers 54

Quantification of protein biomarkers .. 54

Mass spectrometry for quantification of protein biomarkers . 54

Real-time PCR for quantification of protein biomarkers . 54

CyTOF for quantification of biomarkers .. 55

Search for biomarkers in body fluids . 55

Challenges and strategies for discovey of protein biomarkers in plasma . 55

3-D structure of CD38 as a biomarker 56

BD™ Free Flow Electrophoresis System . 57

Isotope tags for relative and absolute quantification .. 57

Plasma protein microparticles as biomarkers 58

Proteome partitioning .. 58

Stable isotope tagging methods 58

Technology to measure both the identity and size of the biomarker .. 59

Biomarkers in the urinary proteome .. 59

Peptides in body fluids and tissues as biomarkers of disease . 60

Analysis of peptides in bodily fluids . 60

Serum peptidome patterns . 61

SISCAPA method for quantitating proteins and peptides in plasma 61

Comparison of proteomic profiling technologies for discovery of biomarkers . 62

Verification for interlaboratory reproducibility of protein biomarkers 62

Significance of similar protein biomarkers in different tissues 63

Glycomic technologies . 63

Metabolomic technologies . 64

Genome-wide as

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