Navigation Links
RNAi Helps Illuminate the Inner Workings of the Cell Cycle: Synthetic RNAissance™ Genes Created by DNA2.0 Enable Key Research
Date:11/13/2008

DNA2.0 has designed RNAi-resistant genes that enabled a breakthrough in understanding the inner workings of the cell cycle. DNA2.0's RNAissance genes are simultaneously optimized for expression in the desired host and to escape inhibition by siRNA that is targeted to the natural gene.

Menlo Park, Calif. (PRWEB) November 13, 2008 -- DNA2.0, the leading synthetic genomics company, announced today that the company's RNAissance genes had been used to shed new light on the mechanisms that ensure that each daughter cell receives a full complement of genetic material when cells divide. The study, published this week in Nature Cell Biology, was a collaboration between DNA2.0 and researchers in Dr. Jonathon Pines' group at the Wellcome/CR UK Gurdon Institute and Department of Zoology at Cambridge.

DNA2.0's RNAissance genes are widely used to ensure that phenotypes obtained when cells are treated with RNAi are not the unintended result of off-target effects. For the work reported in Nature Cell Biology, Dr. Pines went a step further. He made RNAi-resistant versions of both a wildtype and a mutated protein (Cdc20). In the mutated version, all 23 lysines found in the wildtype protein were changed to arginine, a change that prevented the mutant Cdc20 from becoming ubiquitinated. By eliminating the natural Cdc20 from cells using RNAi, the Cambridge group was able to show that ubiquitination of Cdc20 is required to prevent a cell from dividing before all of its chromosomes are lined up on the mitotic spindle. This cell cycle checkpoint is essential to prevent serious genetic damage in animals from yeast to humans.

"We were delighted to help Dr. Pines by designing and making our RNAissance genes," said Dr. Jeremy Minshull, the DNA2.0 coauthor of the study. "Making RNAi-resistant genes that encode mutant versions of cellular proteins has the potential to help us understand the molecula
'/>"/>

Source: PRWeb
Copyright©2008 Vocus, Inc.
All rights reserved

1

GOOD

Page: 1 2 3

Related biology technology :

1. Baxter to Launch GARDian(TM), a Groundbreaking Initiative That Helps Provide Continued Access to GAMMAGARD for Current Patients
2. Edison Innovation Fund Helps Tris Pharma Expand
3. New Cardiology Software Helps Heart Centers Manage for Optimal Clinical Performance
4. Free White Paper Helps Biotech Laboratories Maintain a High Level of Infection Control
5. NASA technology helps predict and prevent future pandemic outbreaks
6. Proteon Therapeutics Appoints Experienced Biopharmaceutical Executive, Gregory D. Phelps to its Board of Directors
7. D-Ribose Helps Energy-Starved Hearts
8. Skin & Aging Article Finds Derma Sciences MEDIHONEY(TM) Helps to Promote Healing Where Antibiotics Fail
9. NASA Nanotechnology-Based Biosensor Helps Detect Biohazards
10. White Paper Helps Hospitals Prepare Staff for Joint Commission Environment of Care Interview Sessions
11. Derma Sciences MEDIHONEY(TM) Helps to Save Patients Limb

Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/2/2009)...2 Reportlinker.com announces that ...atalogue: ,, Drug Discovery Technologies - A G... ,, http://www.reportlinker.com/p0164246/Drug-...-Trends--Opportunities.html ,, Drug discovery ...y pharmaceutical company,s business strategy. Ther...
(Date:12/2/2009)...c. 2 Peregrine Pharmaceu...etion of patient enrollment in a dose confirmation...ith relapsed glioblastoma multiforme (GBM), the de...d monoclonal antibody linked to a radioisotope bei... Cotara specifically targets cells at the center ...
(Date:12/2/2009)...therlands, December 2 Pepscan Hold...y focusing on protein mimicking,technology for the... that it,has appointed Wim E.M. Mol, Ph.D. as new ...e responsible for all of Pepscans corporate functi...tion of the company. He brings to,Pepscan more tha...
(Date:12/2/2009)... 2 S*BIO Pte Ltd today announced d..., SB1518, at The American Society of Hematology 51..., Data will be presented on the clinical result... detailing its safety, tolerability and pharmacoki...ls ranging from 100 to 600 mg. These doses were ad...
Breaking Biology Technology:Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 2Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 3Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 4Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 5Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 6Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 7Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 8Reportlinker Adds Drug Discovery Technologies - A Global Update of Market Trends & Opportunities 9Peregrine Completes Patient Enrollment in Cotara(R) Dose Confirmation and Dosimetry Brain Cancer Trial 2Peregrine Completes Patient Enrollment in Cotara(R) Dose Confirmation and Dosimetry Brain Cancer Trial 3Peregrine Completes Patient Enrollment in Cotara(R) Dose Confirmation and Dosimetry Brain Cancer Trial 4Peregrine Completes Patient Enrollment in Cotara(R) Dose Confirmation and Dosimetry Brain Cancer Trial 5Dutch Biotech Company Pepscan Appoints New CEO 2S*BIO Novel Oral JAK2 Inhibitor SB1518 Demonstrates Safety and Tolerability in Phase 1 Studies for the Treatment of Myeloproliferative and Other Hematological Disorders 2S*BIO Novel Oral JAK2 Inhibitor SB1518 Demonstrates Safety and Tolerability in Phase 1 Studies for the Treatment of Myeloproliferative and Other Hematological Disorders 3S*BIO Novel Oral JAK2 Inhibitor SB1518 Demonstrates Safety and Tolerability in Phase 1 Studies for the Treatment of Myeloproliferative and Other Hematological Disorders 4S*BIO Novel Oral JAK2 Inhibitor SB1518 Demonstrates Safety and Tolerability in Phase 1 Studies for the Treatment of Myeloproliferative and Other Hematological Disorders 5
...evelopment, Biomarker Identification ..., Oct. 10 MultiCell,Technologies, Inc. (OTC Bulle...ve license and technology purchase agreement with ... Company,s Fa2N-4 immortalized,adult human liver c...and has agreed to purchase,certain inventory and e...
...herapeutics Corporation (Nasdaq: TARG ) today ann...of 5,750,000 shares of its common stock at a price...res are being offered by,Targanta. Targanta has gr...ase up to an additional 862,500 shares of common s...ing discounts and commissions, to cover,over-allot...
...efits of Unsaturated Fatty Acids in ... 10 Amarin Corporation plc,(NASDAQ: AMRN ) today...development strategy to capitalize on the known th...ardiovascular disease. Amarin plans to utilize,its...e to develop a series,of products targeting the va...
Other Biology Technology:MultiCell Technologies Signs Worldwide Exclusive License Agreement With Corning Incorporated 2MultiCell Technologies Signs Worldwide Exclusive License Agreement With Corning Incorporated 3Targanta Therapeutics Announces Pricing of its Initial Public Offering 2Amarin Announces Initiation of Cardiovascular Development Strategy 2Amarin Announces Initiation of Cardiovascular Development Strategy 3Amarin Announces Initiation of Cardiovascular Development Strategy 4Amarin Announces Initiation of Cardiovascular Development Strategy 5
(Date:12/3/2009)...els that repels grime and dirt? Expanded battery s...el Aviv University research, just published in Na...sembling peptides at the nano-scale level that cou... a few years. , Operating in the range of 100 na...en smaller, graduate student Lihi Adler-Abramovich...
(Date:12/3/2009)...nality change in warmer water, according to an int...ay make some species more aggressive. , Experime...lia,s Great Barrier Reef have shown for the first ...imid, or consistently bold, and that these individ...eratures rise. , A slight lift of just one or tw...
(Date:12/3/2009)...m the U.S. Department of Energy,s (DOE) Brookhaven...Indian Institute of Science has made a major step ...information in DNA that is necessary to carry out ...ished in the December 1, 2009 edition of Nature S... proteins responsible for interacting at specific ...
Breaking Biology News(10 mins):A window that washes itself? 2Fish with attitude: Some like it hot 2Grooving down the helix 2Grooving down the helix 3Folk Medicine Therapy May Prevent Alcoholic Relapse 54302 1Folk Medicine Therapy May Prevent Alcoholic Relapse 54302 2maxIT Assists Medical Center to Achieve Prestigious Stage 6 Recognition 54299 1maxIT Assists Medical Center to Achieve Prestigious Stage 6 Recognition 54299 2maxIT Assists Medical Center to Achieve Prestigious Stage 6 Recognition 54299 3Members of Over 71 Million U S Households Ignored by Universal Healthcare 54296 1Members of Over 71 Million U S Households Ignored by Universal Healthcare 54296 2Members of Over 71 Million U S Households Ignored by Universal Healthcare 54296 3Members of Over 71 Million U S Households Ignored by Universal Healthcare 54296 4
...f the 2006-2007 activities of UC San Diegos engine...y they recently took home the nations most outstan...oss fundraiser. , Youll find the detailed lesson ...ineering and science fundamentals to 75 fifth grad... find all kinds of information about the tutoring ...
...ajor role in an international scientific team that...ely related fruit fly species. As well has having ...tation to evolving immune systems -- the analysis ...on of each species., From the results of the resea... two papers published this week in the journal Nat...
...y of Idaho researchers are crossing academic and g...ses against Staphylococcus aureus bacteria and oth... effort is to create much faster and more accurate...otic methicillin, formally known as methicillin-re...rough detection technologies are already in hand i...
Other Biology News:UC-San Diego Engineering Honor Society wins most outstanding chapter award 2International team compares 12 fruit fly genomes 2International team compares 12 fruit fly genomes 3Developing kryptonite for Superbug 2Developing kryptonite for Superbug 3Developing kryptonite for Superbug 4Developing kryptonite for Superbug 5
Chaps Cell Extract Buffer (10X) from Cell Signaling Technology
UltraClean Microbial DNA Isolation Kit from Mo Bio Laboratories, Inc.
Custom Oligo Assay Single Locus 1920 samples from Illumina
Anti-Human L-Ficolin Monoclonal Antibody, Unconjugated, Clone GN4 from Cell Sciences
Biology Products: