The terms of the contract call for Precision Antibody to apply its proprietary rapid antibody-generation technology to develop three monoclonal antibodies against each of 20 cancer-related target antigens produced by Argonne National Laboratory that have been identified to be of high interest to the cancer proteomics community. Other terms were not disclosed.
Following screening and initial characterization by Precision Antibody, SAIC-F will undertake screening and additional characterization of the antibodies. Techniques used in the rigorous characterization process may include ELISA and indirect ELISA, Western blot, antibody isotype, SDS-PAGE, surface plasma resonance, immunohistochemistry, immunoprecipitation, immunofluorescence, and immuno-mass spectrometry. Final characterization will be completed by the Biodesign Institute at Arizona State University and by the Royal Institute of Technology in Stockholm, Sweden, which runs the Human Protein Atlas, a comprehensive database that provides protein expression profiles for a large number of human proteins.
Once characterized and produced, NCI's final renewable, highly characterized, high-affinity antibodies and their producing hybridoma cells will be made commercially available to the research community through the Developmental Studies Hybridoma Bank at the University of Iowa. To ensure that experiments can be both repeated and compared among researchers, detailed standard operating procedures will also be provided, along with characterization data that will be available to the scientific community via a web portal (http://antibodies.cancer.gov)
|SOURCE Precision Antibody|
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