Reproducible, Quantitative Fluorescent Imaging Data
Deepak Sharma, Ph.D., senior product manager at Photometrics, will present the Evolve camera line’s advanced features, including those that enable researchers to measure and quantify data in photoelectrons, which allows data generated by any researcher, using any camera, at any time to be directly and quantitatively compared.
Currently, imaging data from EMCCD and CCD cameras is reported in non-standardized units with arbitrary meaning. Researchers cannot use these units (known by many names, including fluorescence units, gray scale values, and analog-to-digital units) to reliably compare or reproduce imaging data between labs—or even within their own.
The problem is that those units are merely electronic representations of incident photons. Photons that hit CCD and EMCCD sensors generate photoelectrons, the fundamental unit of measurement for these devices. These photoelectrons are processed and amplified by the camera in a variable manner, dependent on user-defined settings as well as sensor and camera characteristics. These characteristics differ even between the same make and model of camera. Certain characteristics, such as camera gain values, are in most cases not measured or revealed by camera manufacturers*.
Photometrics’ technology is the first to report imaging data in a camera’s fundamental unit, photoelectrons, not the variable units used by other cameras. Dr. Sharma will explain how this technology and unit of measurement will allow researchers seamlessly reproduce and compare imaging data—a previously impossible task.
The advanced features of the Evolve camera line that Dr. Sharma’s presentation will include are:
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