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Photometrics to Offer Presentations on EMCCD Bio-Imaging Advances at 2010 Biophysical Annual Meeting
Date:2/19/2010

Presentations to Highlight Technologies for Quantitative, Reproducible Imaging and Enhancing Fluorescence Correlation Spectroscopy Data

(PRWEB) February 19, 2010 -- Photometrics, a designer and manufacturer of high-performance CCD and EMCCD cameras for life sciences, will offer two educational presentations at the 2010 Biophysical Annual Meeting. The presentations will cover technologies in the Evolve camera line that markedly improve data quality for fluorescence correlation spectroscopy (FCS) experiments and, for the first time, make fluorescence imaging data reproducible between laboratories and cameras.

Improvements to Fluorescence Correlation Spectroscopy

Researchers use FCS to track diffusion and other fast dynamic properties of molecules, which demand very low noise levels. Methods such as total internal reflectance fluorescence (TIRF) can be used to reduce image background noise and increase signal-to-noise ratio while maintaining good Z-resolution for FCS measurements. EMCCD cameras are often used in such experiments as they can provide higher signal-to-noise ratios than normal CCD cameras.

However, EMCCD cameras have an additional noise source often called “background events” , resulting from the multiplication of spurious events caused by clock induced charge and thermal generated charge. This noise produces what are referred to as “speckles” on the image, resulting in the image containing more noise.

A technology unique to Photometrics Evolve EMCCD cameras, “Background Event Reduction Technology” (BERT), helps improve FCS data clarity and consistency by reducing the “speckles
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Photometrics to Offer Presentations on EMCCD Bio-Imaging Advances at 2010 Biophysical Annual Meeting
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