Navigation Links
Penn researchers show single drug and soft environment can increase platelet production
Date:7/13/2011

on, such as flexing one's muscles. In many cells, it is responsible for the integrity of the outer membranes, for cell division and for key aspects of adhesion. Because megakaryocytes are best when they are large, multi-nucleated and fragment easily, inhibiting myosin-II helps produce more platelets in three distinct ways.

"The first factor is when cells normally divide, there is a contracting force between the dividing cells that cleave them apart," Shin said. "But if you inhibit myosin, there is no contracting force and cells grow without dividing. That's how they become multi-nucleated and how the cell mass becomes bigger.

"The second factor is cytoskeletal stiffness and tension in the cell. When myosin is active, the cell is stiff and tense like well-toned muscle, but if you inhibit the myosin, the cell becomes flaccid and more easy to push around and fragment," he said.

"The third factor is that cells are able to sense the stiffness of their microscopic environment and react to it, which is also regulated by cellular contractivity," Shin said. "Adhering to bone inhibits megakaryocyte growth; without myosin-II, they grow as if they were adhering to something soft."

By testing the megakaryocytes' growth in different cell culture dishes and gels, the researchers were also able to show that a soft matrix, similar to squishy bone marrow, induced more platelet production than a rigid matrix.

After growing the platelets in Petri dishes saturated with the myosin-II inhibitor blebbistatin, the megakaryocytes were soft enough to spontaneously fragment into platelets. Because platelets need functioning myosin-II to form rigid clots, the researchers subsequently washed away the drug to show that the platelets could still activate.

While the researchers also transplanted blebbistatin-treated human megakaryocytes into genetically modified mice to show that they maintained their increased platelet production within l
'/>"/>

Contact: Evan Lerner
elerner@upenn.edu
215-573-6604
University of Pennsylvania
Source:Eurekalert

Page: 1 2 3

Related biology technology :

1. SANYO and BD Biosciences Collaborate to Offer Researchers Valuable Technology and Research Tools
2. TUM researchers develop environmentally friendly process to improve storage stability of probiotics
3. Researchers image graphene electron clouds, revealing how folds can harm conductivity
4. Researchers clarify properties of confined water within single-walled carbon nanotube pores
5. Researchers engineer the environment for stem cell development to control differentiation
6. Penn researchers break light-matter coupling strength limit in nanoscale semiconductors
7. Researchers From More Than 30 Countries Share Findings About the Use of Ultrasound in the Prevention, Diagnosis and Treatment of Heart Disease
8. Singapore researchers invent broadband graphene polarizer
9. Livermore researchers develop battery-less chemical detector
10. Researchers create terahertz invisibility cloak
11. UCLA researchers now 1 step closer to controlled engineering of nanocatalysts
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/12/2014)... (PRWEB) July 12, 2014 ... various reagents used in the life science ... the life science reagents market towards remarkable ... manufacturers and providers of life science reagents. ... Report: http://www.transparencymarketresearch.com/life-sciences-reagents-analytical-reagents.html . , The global ...
(Date:7/11/2014)... BEACH, Fla. , July 11, 2014 /PRNewswire-iReach/ -- ... of high-quality peptides that are used solely in scientific ... honor two successful years in business, Maxim Peptide, which ... has just launched a new and updated website that ... research blog section has been updated with fascinating articles ...
(Date:7/11/2014)... , July 11, 2014 , ... part) in, into or from any jurisdiction where to do ... such jurisdiction.  Shire plc ("Shire" or the "Company") ... article this afternoon.  Shire confirms it has held a meeting ... made by Shire without the prior agreement or approval of ...
(Date:7/11/2014)... 2014 Research and Markets  has announced ... - Global Industry Analysis, Size, Share, Growth, Trends and ... http://photos.prnewswire.com/prnh/20130307/600769 Transfection ... DNA or RNA) into cells. This technology helps the ... and protein metabolism by affecting the nuclear genes. The ...
Breaking Biology Technology:Transparency Market Research: Life Science Reagents Market- PCR Reagent Kits, Cell and Tissue Culture, Flow Cytometry, Electrophoresis and Chromatography 2Transparency Market Research: Life Science Reagents Market- PCR Reagent Kits, Cell and Tissue Culture, Flow Cytometry, Electrophoresis and Chromatography 3Maxim Peptide Celebrates Second Anniversary Online with an Updated Website and Special Deals Section 2Shire plc - Statement re: Media Speculation 2International Transfection Technology Market - Industry Analysis, Size, Share, Growth, Trends and Forecast to 2019 2
... labeling and mass spectrometry-based techniques, Mark Chance, PhD, director ... Center for Proteomics and Bioinformatics and professor of physiology ... Center for Proteomics and Bioinformatics; and a research team ... were able to take a high resolution picture of ...
... , , ... Ormsby reports: , , , ... that the Company has engaged the services of Robert B. Nealon, Esq. as ... in Nealon & Associates, P.C., a Washington, D.C. based law and government relations ...
... association studies have uncovered a number of DNA variants ... risk variants lie outside of genes, posing a challenge ... In a report published online today in ... a functional DNA element associated with prostate cancer, lending ...
Cached Biology Technology:Case Western Reserve University-led team takes high resolution photo of a K+ channel 2Case Western Reserve University-led team takes high resolution photo of a K+ channel 3IGEN Networks engages new Director and Chairman of the Board 2IGEN Networks engages new Director and Chairman of the Board 3Prostate cancer risk variant found to be in a functional DNA sequence linked with disease 2Prostate cancer risk variant found to be in a functional DNA sequence linked with disease 3
(Date:7/11/2014)... A new report from the Wildlife Conservation Society ... only help economically valuable species such as lobster, ... also help re-colonize nearby reef areas. , The ... a systematic review of research literature from no-take ... by Dr. Craig Dahlgren, a recognized expert in ...
(Date:7/11/2014)... Tenn., July 11, 2014--Researchers at the Department of ... R&D 100 awards, presented by R&D Magazine in ... , "These awards recognize the tremendous value of ... Moniz. "Research and development at the National Labs ... challenges and pursue the scientific and technological innovations ...
(Date:7/11/2014)... were many fires burning in eastern New South Wales, ... 11. , At 03:35 UTC on July 11 (12:35 ... Aqua passed over eastern New South Wales (NSW), the ... visible image of the region and spotted smoke (light ... MODIS,s thermal bands, are outlined in red. , The ...
Breaking Biology News(10 mins):Belize's lobster, conch, and fish populations rebuild in no-take zones 2ORNL wins eight R&D 100s 2ORNL wins eight R&D 100s 3ORNL wins eight R&D 100s 4
... for the popular television show "Are You Smarter than a 5th ... The conventional wisdom: rods and cones. The human retina contains ... movement, and about 7 million cones, which in addition detect color. ... not exist. But that might not be true, according to ...
... of all acute leukemias represents a small population of ... standard treatments available today. Though current chemotherapy can produce ... of experiencing a recurrence and dying from the disease. ... rare form of leukemia called acute promyelocytic leukemia (APL), ...
... ANN ARBOR, Mich.---In cells, as in cities, disposing ... is an essential service. In both city and cell, ... New research by University of Michigan cell ... how the cell,s garbage dump and recycling center, the ...
Cached Biology News:Blind mice can 'see' thanks to special retinal cells 2Grant to fund research into eradicating the leukemia stem cell 2Opening the gate to the cell's recycling center 2
... membrane and filter paper sandwiches are composed ... two sheets of thick filter paper that ... filter paper sandwich. These 7 x 8.5 ... immunoblotting fit Ready Gel-sized gels and are ...
... inhibits protein synthesis. Acts by inhibiting ... Reported to induce apoptosis in a ... cells, Jurkat cells, ventricular myocytes, and ... and immediate early gene induction. Selective ...
... The RC DC protein assay kit I provides ... concentration in the presence of reducing agents and ... colorimetric assay. The kit includes reagents and standards ... reagent I, 250 ml RC reagent II, 250 ...
Recommended for use as a cell culture substratum at 0.1-0.2 mg/cm 2 or 5-10 μl/cm 2 . Optimal concentration depends on cell type as well as the application or research objectives. Type B...
Biology Products: