Navigation Links
Optical microscope without lenses produces high-resolution 3-D images on a chip
Date:5/3/2011

UCLA researchers have redefined the concept of a microscope by removing the lens to create a system that is small enough to fit in the palm of a hand but powerful enough to create three-dimensional tomographic images of miniscule samples.

The advance, featured this week in the early online edition of the journal Proceedings of the National Academy of Sciences, represents the first demonstration of lens-free optical tomographic imaging on a chip, a technique capable of producing high-resolution 3-D images of large volumes of microscopic objects.

"This research clearly shows the potential of lens-free computational microscopy," said Aydogan Ozcan, senior author of the research and an associate professor of electrical engineering at UCLA's Henry Samueli School of Engineering and Applied Science. "Wonderful progress has been made in recent years to miniaturize life-sciences tools with microfluidic and lab-on-a-chip technologies, but until now optical microscopy has not kept pace with the miniaturization trend."

An optical imaging system small enough to fit onto an opto-electronic chip provides a variety of benefits. Because of the automation involved in on-chip systems, scientific work could be sped up significantly, which might have a great impact in the fields of cell and developmental biology. In addition, the small size not only has great potential for miniaturizing systems but also leads to cost savings on equipment.

The optical microscope, invented more than 400 years ago, has tended to grow larger and more complex as it has been modified to image ever-smaller objects with better resolution. To address this lack of progress in miniaturization, Ozcan's research group with graduate student Serhan Isikman and postdoctoral scholar Waheb Bishara as lead researchers developed the new tomographic microscopy platform through the next evolution of a lens-free imaging technology the group created and has been improving for years.

Ozcan, a researcher at the California NanoSystems Institute at UCLA, makes the analogy that a traditional optical microscope is like a huge set of pipes delivering content, in the form of images, to the user. Over years of development, bottlenecks occur that impede further improvement. Even if one part of the system that is, one bottleneck is improved, other bottlenecks keep that improvement from being fully realized. Not so with the lens-free system, according to Ozcan.

"Lens-free imaging removes the pipes altogether by utilizing an entirely new design," he said.

The system takes advantage of the fact that organic structures, such as cells, are partially transparent. So by shining a light on a sample of cells, the shadows created reveal not only the cells' outlines but details about their sub-cellular structures as well.

"These details can be captured and analyzed if the shadow is directed onto a digital sensor array," Isikman said. "The end result of this process is an image taken without using a lens."

Ozcan envisions this lens-free imaging system as one component in a lab-on-a-chip platform. It could potentially fit beneath a microfluidic chip, a tool for the precise control and manipulation of sub-millimeter biological samples and fluids, and the two tools would operate in tandem, with the microfluidic chip depositing and subsequently removing a sample from the lens-free imager in an automated, or high-throughput, process.

The platform's 3-D images are created by rotating the light source to illuminate the samples from multiple angles. These multiple angles also allow the system to utilize tomography, a powerful imaging technique. Through the use of tomography, the system is able to produce 3-D images without sacrificing resolution.

"The field of view of lens-based microscopes is limited because the lens focuses on a narrow area of a sample," Bishara said. "A lens-free microscope has both a much larger field of view and depth of field because the imaging is done by the digital sensor array and is not constrained by a lens."


'/>"/>

Contact: Mike Rodewald
mrodewald@cnsi.ucla.edu
310-267-5883
University of California - Los Angeles
Source:Eurekalert

Related biology technology :

1. University of Pennsylvania scientists move optical computing closer to reality
2. SpectraScience Awarded Patent for Its Optical Biopsy System and Methods for Tissue Diagnosis
3. Paperwork: Buckypapers clarify electrical, optical behavior of nanotubes
4. New research field promises radical advances in optical technologies
5. Nanoscale dimensioning is fast, cheap with new NIST optical technique
6. Finesse Demonstrates TruFluor(TM) pH, a Single-Use Optical pH Sensor and Transmitter
7. Shamir Optical Industry Ltd. Announces Availability of Its Annual Report on Form 20-F Through Its Website
8. New nanolaser key to future optical computers and technologies
9. Reportlinker Adds Worldwide Optical, Transmission TEM, and Scanning SEM Electron Microscope Market Shares, Strategies, and Forecasts, 2009 to 2015
10. Small optical force can budge nanoscale objects
11. An optical traffic cop for rapid communication
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/24/2017)... As Ebola resurfaces in the Democratic Republic of ... now reported, a new analysis of the Ebola gene polymerase ... the 2014 and 2017 outbreaks of the disease.  ... preceded the 2014 outbreak. An analysis of the latest data ... 2014-15, which again precedes the current outbreak in the DRC. ...
(Date:5/23/2017)... (PRWEB) , ... May 23, 2017 , ... ... celebrates this month its 20th anniversary, marking the occasion with a strong presence ... the meeting’s Welcome Reception and further extends an invitation to all attendees to ...
(Date:5/23/2017)... ... 23, 2017 , ... Energetiq Technology, a world leader in ... to accommodate its rapid growth. , The renovations at the company’s headquarters in ... existing areas. The expansion includes, a state-of-the-art engineering facility, and a second clean ...
(Date:5/23/2017)... ... May 23, 2017 , ... ... re-engineer their control technology again and again. METTLER TOLEDO has released two new ... The videos illustrate how integration of the ACT350 into Siemens and Allen Bradley ...
Breaking Biology Technology:
(Date:4/5/2017)... April 5, 2017 Today HYPR Corp. ... the server component of the HYPR platform is officially ... the end-to-end security architecture that empowers biometric authentication across ... has already secured over 15 million users across the ... of connected home product suites and physical access represent ...
(Date:3/30/2017)...  On April 6-7, 2017, Sequencing.com will host the ... hackathon at Microsoft,s headquarters in Redmond, Washington ... developing health and wellness apps that provide a unique, ... is the first hackathon for personal genomics and ... in the genomics, tech and health industries are sending ...
(Date:3/28/2017)... 28, 2017 The report "Video ... Monitors, Servers, Storage Devices), Software (Video Analytics, VMS), and ... Global Forecast to 2022", published by MarketsandMarkets, the market ... is projected to reach USD 75.64 Billion by 2022, ... The base year considered for the study is 2016 ...
Breaking Biology News(10 mins):