Navigation Links
On the trail of rogue genetically modified pathogens
Date:3/17/2008

Bacteria can be used to engineer genetic modifications, thereby providing scientists with a tool to combat many challenges in areas from food production to drug discovery. However, this sophisticated technology can also be used maliciously, raising the threat of engineered pathogens. New research published in the online open access journal Genome Biology shows that computational tools could become a vital resource for detecting rogue genetically engineered bacteria in environmental samples.

Jonathan Allen, Shea Gardner and Tom Slezak of the Lawrence Livermore National Laboratory in California, US, designed new computational tools that identify a set of DNA markers that can distinguish between artificial vector sequences and natural DNA sequences. Natural plasmids and artificial vector sequences have much in common, but these new tools show the potential to achieve high sensitivity and specificity, even when detecting previously unsequenced vectors in microarray-based bioassays.

A new computational genomics tool was developed to compare all available sequenced artificial vectors with available natural sequences, including plasmids and chromosomes, from bacteria and viruses. The tool clusters the artificial vector sequences into different subgroups based on shared sequence; these shared sequences were then compared with the natural plasmid and chromosomal sequence information so as to find regions that are unique to the artificial vectors. Nearly all the artificial vector sequences had one or more unique regions. Short stretches of these unique regions are termed candidate DNA signatures and can be used as probes for detecting an artificial vector sequence in the presence of natural sequences using a microarray. Further tests showed that subgroups of candidate DNA signatures are far more likely to match unseen artificial than natural sequences.

The authors say that the next step is to see whether a bioassay design using DNA signatures on microarrays can spot genetically modified DNA in a sample containing a mixture of natural and modified bacteria. The scientific community will need to cooperate with computational experts to sequence and track available vector sequences if DNA signatures are to be used successfully to support detection and deterrence against malicious genetic engineering applications. Scientists would be able to maintain an expanding database of DNA signatures to track all sequenced vectors.

As with any attempt to counter malicious use of technology, detecting genetic engineering in microbes will be an immense challenge that requires many different tools and continual effort, says Allen.


'/>"/>

Contact: Charlotte Webber
charlotte.webber@biomedcentral.com
44-020-763-19980
BioMed Central
Source:Eurekalert

Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/23/2016)... , June 23, 2016 Houston ... with the Cy-Fair Sports Association to serve as ... the agreement, Houston Methodist Willowbrook will provide sponsorship ... and connectivity with association coaches, volunteers, athletes and ... with the Cy-Fair Sports Association and to bring ...
(Date:6/23/2016)... June 23, 2016   EpiBiome , a precision ... million in debt financing from Silicon Valley Bank (SVB). ... and to advance its drug development efforts, as well ... "SVB has been an incredible strategic partner ... a traditional bank would provide," said Dr. Aeron ...
(Date:6/23/2016)... LOUISVILLE, Ky. , June 23, 2016 /PRNewswire/ ... from two Phase 1 clinical trials of its ... double-blind, placebo-controlled, single and multiple ascending dose studies ... and pharmacodynamics (PD) of subcutaneous injection in healthy ... APL-2 subcutaneously (SC) either as a single dose ...
(Date:6/23/2016)... ... 23, 2016 , ... ClinCapture, the only free validated electronic ... showcase its product’s latest features from June 26 to June 30, 2016 for ... Disrupting Clinical Trials in The Cloud during the conference. DIA (Drug Information ...
Breaking Biology Technology:
(Date:6/22/2016)... BETHESDA, Md. , June 22, 2016  The American ... by Trade Show Executive Magazine as one of ... Summit on May 25-27 at the Bellagio in ... based on the highest percentage of growth in each of ... number of exhibiting companies and number of attendees. The 2015 ...
(Date:6/16/2016)... 16, 2016 The global ... to reach USD 1.83 billion by 2024, according ... Inc. Technological proliferation and increasing demand in commercial ... to drive the market growth.      ... The development of advanced multimodal techniques for biometric ...
(Date:6/3/2016)... LONDON , June 3, 2016 /PRNewswire/ ... Transport Management) von Nepal ... ,Angebot und Lieferung hochsicherer geprägter Kennzeichen, einschließlich ... weltweit führend in der Produktion und Implementierung ... an der Ausschreibung im Januar teilgenommen, aber ...
Breaking Biology News(10 mins):