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Nucleic Acids Research Journal Publishes Sapphire Energy Peer-Reviewed Research Paper on a Chloroplast Genome From Green Algae
Date:12/5/2011

SAN DIEGO, Dec. 5, 2011 /PRNewswire/ -- Sapphire Energy, Inc., one of the world leaders in algae-based crude oil, today announced that Nucleic Acids Research Journal has published its white paper, "An exogenous chloroplast genome for complex sequence manipulation in algae."  As the site of photosynthesis in plants and algae, the chloroplast is responsible for producing organic molecules from atmospheric carbon dioxide (CO2) and is thus a crucial metabolic engineering target.  Sapphire Energy cultivates algae that use sunlight and CO2 to create Green Crude, a low carbon renewable crude oil substitute that can be refined into jet fuel, diesel and gasoline.

This white paper (http://bit.ly/tfgaMI)  describes methodology for the design, construction, modification, and cellular introduction of the chloroplast genome from the green alga, Chlamydomonas reinhardtii.  Using this methodology, the authors simultaneously modify multiple independent regions, including genes that encode core subunits of the photosynthetic apparatus, in the chloroplast genome of living algae cells.  This work should allow for the creation of genetic diversity in all regions of a chloroplast genome, potentially in any photosynthetic organism. 

"With this breakthrough, Sapphire Energy has shown that it is possible to make algae--the world's most efficient photosynthetic organism--even more efficient," said Jason Pyle, Sapphire Energy founder and CEO.  "This work represents the first steps toward a novel approach for creating genetic diversity in any or all regions of a chloroplast genome, and may have applications in other plants."  

Chloroplast genomes present a unique opportunity for the field of synthetic biology.  In a single, relati
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SOURCE Sapphire Energy, Inc.
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