Navigation Links
Novel surface triples stem-cell growth in culture
Date:11/8/2011

FINDINGS: By irradiating typical polystyrene lab plates with ultraviolet (UV) waves, Whitehead Institute and MIT scientists have created a surface capable of tripling the number of human embryonic stem (ES) and induced pluripotent stem (iPS) cells that can be grown in culture by current methods. Moreover, use of this novel surface eliminates the need for layers of mouse "feeder cells" to support ES- and iPS-cell growth.

RELEVANCE: Because of their ability to regenerate and to become almost any cell type in the body, ES and iPS cells hold promise for studying diseases, such as Parkinson's and Alzheimer's, screening potential drugs for these diseases, and possibly creating therapeutic cells for transplantation into patients with diabetes, neurodegenerative diseases, or nerve or other tissue damage. Such techniques could require millions or even billions of cells, but current cell cultivation methods act as a bottleneck and are unable to efficiently produce such large numbers of cells.

CAMBRIDGE, Mass. (Nov. 7, 2011) By irradiating typical polystyrene lab plates with ultraviolet (UV) waves, Whitehead Institute and MIT scientists have created a surface capable of tripling the number of human embryonic stem (ES) and induced pluripotent stem (iPS) cells that can be grown in culture by current methods. Use of this novel surface also eliminates the need for layers of mouse "feeder cells" to support ES- and iPS-cell growth.

"Polystyrene is the most common cell culture surface used in labs, and to be able to do a simple treatment and get something that works better than the mouse feeder layers is great and potentially has a lot of utility," says Daniel Anderson, Associate Professor in the Harvard-MIT Division of Health Sciences & Technology.

The research is published online this week in the Proceedings of the National Academy of Sciences (PNAS).

The promise of ES and iPS cells lies in their ability to regenerate and to become almost any cell type in the body. By harnessing this ability, scientists can use ES and iPS cells to create cell lines that model difficult-to-study diseases, such as Parkinson's and Alzheimer's, and screen for potential drugs for these diseases. Also, researchers may one day manipulate iPS cells into therapeutic cells for transplantation into patients with diabetes, neurodegenerative diseases, and nerve or other tissue damage.

Some scientists estimate that such techniques would require millions or even billions of cells, but current cell cultivation methods thwart efficient production of such mass quantities. Current approaches to cell culturing also rely on supportive mouse feeder cells, which can contaminate the stem cells by transferring viruses or macromolecules from the mouse cells to the human cells. The resulting cells would be deemed unsuitable for transplantation into humans.

To address both the bottleneck in stem cell cultivation and the potential risk for contamination, Krishanu Saha and Ying Mei, co-first authors of the PNAS paper, have been investigating what ES and iPS cells require to grow quickly on a lab plate. In a Nature Materials paper in 2010, Mei and Saha reported inducing significant increases in cell growth by using synthetic polymer substrates with high content of hydrocarbon and ester groups. Although successful, that process is less practical for large-scale production because the required polymers are difficult to synthesize in various cell culture formats.

"The last paper is trying to figure out what is the correct, optimum surface chemistry to support stem cells," says Mei, a research associate in the labs of Robert Langer and Daniel Anderson in MIT's chemical engineering department. "This paper says, with the knowledge we have about this optimal surface chemistry, what is the easiest way and the most reasonable, cost-effective way to generate this surface chemistry so we can make dishes efficiently grow stem cells."

In the current paper, Saha and Mei have taken an unconventional route to producing the ideal concentration of hydrocarbon and ester groups on the surface of the polystyrene platesirradiating them with UV waves from a UV ozone unit in the MIT chemical engineering department. Saha and Mei found that a mere 2.5 minutes of UV irradiation produced the impressive growth rates in the absence of feeder cells.

"I think it's going to be a useful technique," says Saha, who is a postdoctoral researcher in Whitehead Member Rudolf Jaenisch's lab. "There is a lot of push, at least in the field, to [eliminate animal products]. If you were one day to inject these cells into patients, you wouldn't have to worry about as many safety risks as if you had co-cultured them with animal cells."


'/>"/>
Contact: Nicole Giese
giese@wi.mit.edu
617-258-6851
24/7 Consulting
Source:Eurekalert

Related biology technology :

1. Epitomics, Inc. Receives Notice of Allowance from the United States Patent and Trademark Office for a Novel Method for Identifying Differentially Expressed Proteins
2. Silence Therapeutics Signs Collaboration With Mirna Therapeutics to Evaluate Delivery of Novel microRNA Therapeutics
3. Oxford BioMedica Announces US IND Approval for Novel Ocular Product in Usher Syndrome Type 1B
4. Carbon nanotube muscles generate giant twist for novel motors
5. Cleave Biosciences Raises $42 Million in Series A Financing for Novel Cancer Therapies
6. Wideband Antenna for Novelda Nanoscale Impulse Radar
7. Roche Researchers Sequence Complete Genome of Cynomolgus Monkey and Develop Novel Gene Expression Microarray for Drug Safety Assessment
8. SanBio Announces Site Opening of Phase 1/2a Clinical Trial of Novel Cell Therapy in Stable Stroke Patients at University of Pittsburgh Medical Center
9. ALS Therapy Development Institute and Aestus Therapeutics, Inc., Expand Collaboration on Development of Novel ALS Therapies
10. Stevens researchers pioneer novel technique to make plasmonic nanogap arrays
11. Amsterdam Molecular Therapeutics Licenses Additional Novel Gene Therapy Vectors from National Institutes of Health
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/9/2017)... ... 09, 2017 , ... At its national board meeting in North Carolina, ... University’s Departments of Physics and Astronomy, has been selected for membership in ARCS ... for the 2015 Breakthrough Prize in Fundamental physics for the discovery of the accelerating ...
(Date:10/7/2017)... (PRWEB) , ... October 06, ... ... years’ experience providing advanced instruments and applications consulting for microscopy and surface ... expertise in application consulting, Nanoscience Analytical offers a broad range of contract ...
(Date:10/6/2017)... Mass. (PRWEB) , ... October 06, 2017 , ... ... female entrepreneurship within the healthcare and technology sector at their fourth annual Conference ... panels featuring 30 inspiring speakers and the ELEVATE pitch competition showcasing early stage ...
(Date:10/5/2017)... ... ... Understanding the microbiome, the millions of bacteria that live in our guts, is ... the newest exhibit on display at the University City Science Center’s Esther Klein Gallery ... of the gut microbiome. , Gut Love opens October 12, 2017, and runs ...
Breaking Biology Technology:
(Date:4/11/2017)... 11, 2017 Crossmatch®, a globally-recognized leader ... today announced that it has been awarded a ... Activity (IARPA) to develop next-generation Presentation Attack Detection ... "Innovation has been a driving force within Crossmatch ... allow us to innovate and develop new technologies ...
(Date:4/11/2017)... MELBOURNE, Florida , April 11, 2017 ... "Company"), a security technology company, announces the appointment of independent ... John Bendheim to its Board of Directors, furthering the ... ... behalf of NXT-ID, we look forward to their guidance and ...
(Date:4/5/2017)... -- Today HYPR Corp. , leading innovator in ... the HYPR platform is officially FIDO® Certified . ... that empowers biometric authentication across Fortune 500 enterprises and ... 15 million users across the financial services industry, however ... suites and physical access represent a growing portion of ...
Breaking Biology News(10 mins):