Navigation Links
Novel chip-based platform could simplify measurements of single molecules
Date:8/14/2014

Researchers at UC Santa Cruz have developed a new approach for studying single molecules and nanoparticles by combining electrical and optical measurements on an integrated chip-based platform. In a paper published July 9 in Nano Letters, the researchers reported using the device to distinguish viruses from similarly-sized nanoparticles with 100 percent fidelity.

Combining electrical and optical measurements on a single chip provides more information than either technique alone, said corresponding author Holger Schmidt, the Kapany Professor of Optoelectronics in the Baskin School of Engineering and director of the W. M. Keck Center for Nanoscale Optofluidics at UC Santa Cruz. Graduate student Shuo Liu is first author of the paper.

The new chip builds on previous work by Schmidt's lab and his collaborators at Brigham Young University to develop optofluidic chip technology for optical analysis of single molecules as they pass through a tiny fluid-filled channel on the chip. The new device incorporates a nanopore that serves two functions: it acts as a "smart gate" to control the delivery of individual molecules or nanoparticles into the channel for optical analysis; and it allows electrical measurements as a particle passes through the nanopore.

"The nanopore delivers a single molecule into the fluidic channel, where it is then available for optical measurements. This is a useful research tool for doing single-molecule studies," Schmidt said.

Biological nanopores, a technology developed by coauthor David Deamer and others at UC Santa Cruz, can be used to analyze a DNA strand as it passes through a tiny pore embedded in a membrane. Researchers apply voltage across the membrane, which pulls the negatively charged DNA through the pore. Current fluctuations as the DNA moves through the pore provide electrical signals that can be decoded to determine the genetic sequence of the strand.

With the new device, researchers are able to gather electrical measurements on a nanoparticle as it moves through a pore in a solid membrane, and then measure the optical signals when the particle encounters a beam of light in the channel. By correlating the strength of the current decrease as a particle moves through the pore, the intensity of the optical signal, and the time of each measurement, the researchers are able to discriminate among particles with different sizes and optical properties and to determine the flow speed of particles through the channel.

The chip can also be used to differentiate particles of similar size but different composition. In one experiment, the researchers combined influenza viruses with nanobeads of a similar diameter and placed the mixture above the nanopore. The virus was labeled with a red fluorescent tag and the beads were labeled with a blue tag. The researchers correlated the electrical signal with the fluorescent wavelength and the time of each measurement. They found that the blue nanobeads traveled faster through the channel than red influenza virus, perhaps because of a difference in surface charge or mass. Besides identifying pathogens in a mixture, the researchers can also count the number of virus particles.

"This could be used as an analytical device to do reliable counts of virus particles in a sample," Schmidt said.

Currently, Schmidt's group is working on methods to add optical trapping to the device. This would allow a molecule in the channel to be held in one place, investigated, and released, with the potential to analyze hundreds of molecules in an hour. "Having this all on one chip would make single-molecule measurements much easier and more convenient," Schmidt said.


'/>"/>

Contact: Tim Stephens
stephens@ucsc.edu
831-459-2495
University of California - Santa Cruz
Source:Eurekalert

Related biology technology :

1. Synthetic Biologics Academic Collaborator to Present Novel Monoclonal Antibody Combination for Treatment of Whooping Cough (Pertussis) at 54th ICAAC
2. Diagenode Launches Novel CRISPR/Cas9 Monoclonal Antibody
3. Harnessing Novel Techniques to Beat Dreadful Diabetes
4. University of Illinois researchers demonstrate novel, tunable nanoantennas
5. Novel Ms Techniques Speed Detection of Biopharmaceutical Product and Process Impurities, New Webinar Hosted by Xtalks
6. Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information
7. Sequenom Announces Issuance Of European Patent For Novel Methods Of Detecting Fetal Aneuploidy
8. Data Highlights BGB324 as a Potential Novel Treatment Option for Drug Resistant Chronic Myeloid Leukemia
9. NKT Therapeutics Identifies Novel anti-iNKT Antibodies for Depleting or Activating iNKT Cells
10. Phase 2a Findings Demonstrate that CSL112, A Novel Apolipoprotein A-I Infusion Therapy, Has a Favorable Safety Profile, is Well Tolerated and Increases Cholesterol Efflux Capacity in Stable Atherothrombotic Patients
11. SignalRX Pharmaceuticals Issued U.S. Patent Covering Novel Kinase Inhibitors For Treating Cancer and Other Diseases
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/23/2016)... WA (PRWEB) , ... June 23, 2016 , ... ... announces the release of its second eBook, “Clinical Trials Patient Recruitment and Retention ... recruitment and retention in this eBook by providing practical tips, tools, and strategies ...
(Date:6/23/2016)...   Boston Biomedical , an industry leader ... target cancer stemness pathways, announced that its lead ... Designation from the U.S. Food and Drug Administration ... gastroesophageal junction (GEJ) cancer. Napabucasin is an orally ... stemness pathways by targeting STAT3, and is currently ...
(Date:6/23/2016)... , June 23, 2016 A person commits a ... crime scene to track the criminal down. An ... Food and Drug Administration (FDA) uses DNA evidence to track ... Sound far-fetched? It,s not. The FDA has increasingly ... support investigations of foodborne illnesses. Put as simply as possible, ...
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, Inc. ... test has received AOAC Research Institute approval 061601. , “This is another AOAC-RI ... stated Bob Salter, Vice President of Regulatory and Industrial Affairs. “The Peel Plate ...
Breaking Biology Technology:
(Date:6/9/2016)... 2016  Perkotek an innovation leader in attendance control systems is proud to announce ... for employers to make sure the right employees are actually signing in, and to ... ... ... ...
(Date:6/2/2016)... , June 2, 2016 The Department ... has awarded the 44 million US Dollar project, for the ... Vehicle Plates including Personalization, Enrolment, and IT Infrastructure , ... in the production and implementation of Identity Management Solutions. Numerous ... however Decatur was selected for the ...
(Date:5/24/2016)... , May 24, 2016 Ampronix facilitates superior patient care by providing unparalleled ... medical LCD display is the latest premium product recently added to the range of ... ... ... Sony 3d Imaging- LCD Medical Display- Ampronix News ...
Breaking Biology News(10 mins):