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Nikon Instruments Launches Fully-Automated A1 Confocal Laser Microscope Series
Date:1/29/2008

l and spatial resolution of intermolecular interaction. This is done using the standard galvanometer scanner for stimulation, while the high-speed resonant scanner captures the image. Ultra stable clock sync pulses are generated optically, offering images that are completely even in intensity without flicker or distortion, even at the highest speed. Analysis software for FRAP and FRET is also provided. The A1 system without the resonant scanner provides all the unique features of the A1R minus the high speed resonant scanner.

Among the A1's advanced features are three beam introduction ports, which allow the connection of two fiber coupled laser sets and one air space coupled laser. The AOTF modulated 4-laser unit, which provides as many as seven laser lines (choices from 405nm, 440nm, 457nm, 488nm, 514nm, 543nm, 562nm and 638nm), the AOM modulated 3-laser unit, which provides an additional three laser lines, and an optional picosecond or faster pulsed laser are just three of the laser options available. Laser power for two of the input ports are continuously monitored and reported to the control system ensuring excellent quantitative and uniform imaging performance.

There are also three output ports to allow optical fiber connection to as many as three separate detector units. Specifically, the A1 features a new optional spectral detector for concurrent acquisition of up to 32 channels. Three spectral resolutions or channel widths are available (2.5nm, 6nm and 10nm), simultaneously covering up to 320nm of spectrum at each frame scan. Simultaneous excitation using up to four laser lines can be achieved with frames speeds of two fps at 512 x 512 pixel resolution, or as fast as 16 fps at 512 x 64 pixels. Nikon's original linear unmixing algorithms and high- speed data processing enable fast and accurate unmixing during image acquisition in less than a second. When coupled with high-speed spectral imaging, an image with no crosstalk or auto-fluorescence c
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SOURCE Nikon Instruments, Inc.
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