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Nikon Corporation Acquires License From Harvard University For 'STORM' Super Resolution Microscopy -- Will Create Innovative New 'N-STORM' Microscope
Date:12/3/2009

s. Using this concept, two- and three-dimensional, multicolor fluorescence images of molecular complexes, cells and tissues with a few tens of nanometers resolution has been achieved. This new form of fluorescence microscopy allows molecular interactions in cells and cell-cell interactions in tissues to be imaged at the nanometer scale.

An order of magnitude better resolution than conventional optical microscopes

N-STORM is based on the world renowned Nikon Eclipse Ti research inverted microscope incorporating CFI60 objectives featuring high numerical apertures developed using unique optical design, coatings and manufacturing techniques. N-STORM provides dramatically enhanced resolution of that is 10 times or better than that of conventional optical microscopes. The N-STORM instrumentation will be capable of multi-spectral two-dimensional and three-dimensional nanoscopy, with lateral resolution to approximately 20nm and axial resolution to approximately 50nm, extending the role of the optical microscope to near molecular level resolution.

Image construction by overlaying single molecule images

The STORM method is new technology that reconstructs high resolution fluorescence images (2D and 3D) from localization information of fluorophores detected with high accuracy and calculated from multiple exposures. It generates much more information from detection of single molecule fluorescence emissions and goes one step further, from structural to molecular understanding of the specimen.

Three-dimensional information capability

The N-STORM Super Resolution microscope system will not only provide high-resolution two-dimensional image acquisition capability, but with the addition of a 3D optical device switchover built in the microscope, it will also acquire multi-color high-resolution fluorescence images of the same specimen in 3D, without the need for time consuming serial section acqui
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SOURCE Nikon Instruments Inc.
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