New technique produces genetically identical ...(ause all cells in the mouse contain the ...)
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New technique produces genetically identical stem cells
Date:7/1/2008
ause all cells in the mouse contain the same number of viral integrations in the same location within the genome. With previous approaches, each reprogrammed cell differed because the viruses used to insert the reprogramming genes could integrate anywhere in the cell's DNA with varying frequency.
Wernig and Lengner's method also increases the reprogramming efficiency from one in a thousand cells to one in twenty.
The large numbers of IPS cells that can be created by this method can aid experiments requiring millions of identical cells for reprogramming, such as large-scale chemical library screening assays.
"In experiments, the technique will eliminate many of the reprogramming process's unpredictable variables and simplify enormously the research on the reprogramming mechanism and the screening for virus replacements," says Jaenisch, who is also a professor of biology at Massachusetts Institute of Technology.
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| Contact: Cristin Carr carr@wi.mit.edu 617-324-0460 Whitehead Institute for Biomedical Research Source:Eurekalert |
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