Navigation Links
New technique allows simultaneous tracking of gene expression and movement
Date:12/16/2008

Flies expressing green fluorescent protein (GFP) in their retina cells or other tissues can be tracked by specially modified video cameras, creating a real time computer record of movement and gene expression. The new technique, described in the open access journal BMC Biotechnology, will allow detailed analyses of correlations between behavior, gene expression and aging.

John Tower led a team of researchers from the University of Southern California, Los Angeles, who carried out the fluorescent experiments in Drosophila flies. When the flies are illuminated with blue light, the authors' video tracking system allows tissue-specific GFP expression to be visualized, then quantified and correlated with 3D animal movement in real time. According to Tower, "These methods allow specific temporal patterns of gene expression to be correlated with temporal patterns of animal activity, behavior and mortality".

The green fluorescent protein gene is isolated from the jellyfish Aequorea victoria and encodes a protein that absorbs blue light and emits green light. When a fly expressing GFP is illuminated by blue LEDs, filtered cameras can detect the green fluorescence that results and the fly's movement can be tracked at a rate of 60 frames per second. By linking the expression of GFP to the expression of other reporter genes, it is possible to determine when these genes are on or off, and how this is associated with a fly's behavior.

Tower said, "A large number of strains exist where GFP or some other auto-fluorescent protein is used as a reporter for specific gene expression in Drosophila and other organisms. -Our methods should be readily adaptable to such reagents, for example we have recently been successful in tracking DsRED fluorescent flies".


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-079-205-86437
BioMed Central
Source:Eurekalert

Page: 1

Related biology technology :

1. New technique producing small things in large quantities
2. New DNA-based technique for assembly of nano- and micro-sized particles
3. NIH Announces Advanced Cell Technologys Single Cell Embryo Biopsy Technique as a Means to Derive Embryonic Stem Cells to be Considered for Federal Funding
4. New technique can be breakthrough for early cancer diagnosis
5. University of Leicester scientists discover technique to help friendly bacteria
6. Glycominds Joins Biomolecular Photonic (BMP) Consortium to Develop New Molecular Imaging Technique
7. Doping technique brings nanomechanical devices into the semiconductor world
8. Cardica and Intuitive Surgical to Host Educational Symposium on Advanced Techniques and Technologies in Robotic Coronary Revascularization and Mitral Valve Repair
9. DNA technique yields 3-D crystalline organization of nanoparticles
10. Unique infrared technique finds applications in nanoscience
11. Assembly technique for tiny wires may eventually help detect cancer and other diseases
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/15/2014)... , Dec. 15, 2014  GlassesOff Inc. (OTCBB: GLSO) ... as an independent director of the Company,s Board ... AeroScout and served as its CEO until its acquisition ... a $240 million transaction. Recognized as the inventor of ... leadership, AeroScout provided Wi-Fi -based RFID solutions focused on ...
(Date:12/13/2014)... CHARLOTTE, N.C. (PRWEB) December 13, 2014 ... (UNC Charlotte) spin-out company has changed its name to ... more accurate representation of where we are today,” said ... “We’ve extended our platform’s applications beyond breast cancer diagnostics ... is a reflection of that evolution.” , Founded in ...
(Date:12/13/2014)... The DNA Microarray market market ... is expected to grow at a CAGR of ... the TOC of the DNA Microarray for an ... with help of various tables and figures. , ... market consists of instrument, consumables, and services, where ...
(Date:12/13/2014)... According to population studies: “North Carolina ... and 2030." If a population explosion like that actually ... state in the country. , To that end, North ... of North Carolina Forum on December 16th to ask ... necessary infrastructure in place? Enough clean water? And will ...
Breaking Biology Technology:AeroScout Founder Joins GlassesOff's Board of Directors 2AeroScout Founder Joins GlassesOff's Board of Directors 3CanDiag Inc. Expands Product Scope Under a New Name: OncoTab, Inc. 2DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 2DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 4North Carolina Chamber of Commerce: Asking Questions & Gathering Answers with a Futurist 2
... years, dieters and researchers around the world have seen the ... Gonadotropin) supplements can have on the battle against obesity. Now, ... Bioscience, a leader in diet support products , dieters ... of the powerful metabolic boost of hCG without unwanted hormones ...
... Inc. (NYSE Amex: PIP ) announced today ... of its second generation anthrax vaccine, SparVax™.  In addition ... scale and demonstrating 36 month stability, the Company announced ... engineering production run for SparVax™.  As a result, PharmAthene ...
... By looking to Mother Nature for solutions, researchers ... battery electrodes that not only could boost energy storage, ... used to manufacture the components. Known as alginate, ... In tests so far, it has helped boost energy ...
Cached Biology Technology:Hormone-free hCG Zero Capsules™ Boost Metabolism to Enhance Weight Loss Without Starvation 2Hormone-free hCG Zero Capsules™ Boost Metabolism to Enhance Weight Loss Without Starvation 3Hormone-free hCG Zero Capsules™ Boost Metabolism to Enhance Weight Loss Without Starvation 4PharmAthene Achieves Significant Progress in SparVax™ Anthrax Vaccine Program 2PharmAthene Achieves Significant Progress in SparVax™ Anthrax Vaccine Program 3PharmAthene Achieves Significant Progress in SparVax™ Anthrax Vaccine Program 4Powered by seaweed: Polymer from algae may improve battery performance 2Powered by seaweed: Polymer from algae may improve battery performance 3Powered by seaweed: Polymer from algae may improve battery performance 4
(Date:12/17/2014)... 2014 Research and Markets ( ... "Global Chemical Sensor Market 2015-2019" report ... http://photos.prnewswire.com/prnh/20130307/600769 One major trend upcoming ... medical sensors in biomedical applications. Chemical sensors help ... correct diagnosis during surgical procedures. The Global Chemical ...
(Date:12/11/2014)... , Dec. 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced ... India 2015-2019" report to their offering. ... market is the adoption of multimodal biometric systems. ... of an individual for verification and identification purposes. ...
(Date:12/10/2014)... , Dec. 9, 2014  Valencell, a leader in ... seeing a staggering demand from its licensees for highly ... revealed, is not solely coming from fitness and health ... "A wearable is only as useful as ... is the ultimate driver in long-term mass consumer adoption ...
Breaking Biology News(10 mins):Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2Wearable Technology Products Demand Highly Accurate Biometric Technology 2
... took the wraps off a new,line of sleek, powerful, ... Weighing just over 3 pounds, the VAIO Z model ... LCD,technology for scratch-resistant durability and 100 percent color,saturation., ... display,providing a 40 percent wider workspace than a standard ...
... The Ecological Society of America (ESA) will present societal ... ESA meeting in Milwaukee, Wisconsin on Monday, August 4, ... , Michael Rosenzweig of the University of Arizona is ... to a senior ecologist in recognition of an outstanding ...
... Society for Microbiology (ASM) Procter & Gamble Award ... to Edward F. DeLong, Professor, Massachusetts Institute of ... and development in applied and environmental microbiology. ... in the field of marine microbiology. His work ...
Cached Biology News:New Sony PC Delivers High Performance in Ultra-Portable Package 2New Sony PC Delivers High Performance in Ultra-Portable Package 3Ecological Society of America announces 2008 award recipients 2Ecological Society of America announces 2008 award recipients 3Ecological Society of America announces 2008 award recipients 4American Society for Microbiology honors Edward F. DeLong 2
... At the forefront of Agilent analytical solutions ... local area networking (LAN) allows you to ... fast and informed decision making. Just as ... all the flexibility and performance needed for ...
The novel and unique HCTplus delivers unmatched information-rich data-dependent LC/MSn data for todays challenge in proteomics and metabonomics work....
The essence of fluorescence analysis is sensitivity. The high throughput optical system in the RF-5301PC employs a blazed holographic grating, photomultiplier and digital signal processing to provide...
... In Situ Assay Kit is a fluorescent-based ... cells undergoing apoptosis. The kit is for ... diagnostic or therapeutic procedures. CHEMICON®s CaspaTag™ Pan-Caspase ... approach to detect active caspases. The methodology ...
Biology Products: