Navigation Links
New technique allows simultaneous tracking of gene expression and movement
Date:12/16/2008

Flies expressing green fluorescent protein (GFP) in their retina cells or other tissues can be tracked by specially modified video cameras, creating a real time computer record of movement and gene expression. The new technique, described in the open access journal BMC Biotechnology, will allow detailed analyses of correlations between behavior, gene expression and aging.

John Tower led a team of researchers from the University of Southern California, Los Angeles, who carried out the fluorescent experiments in Drosophila flies. When the flies are illuminated with blue light, the authors' video tracking system allows tissue-specific GFP expression to be visualized, then quantified and correlated with 3D animal movement in real time. According to Tower, "These methods allow specific temporal patterns of gene expression to be correlated with temporal patterns of animal activity, behavior and mortality".

The green fluorescent protein gene is isolated from the jellyfish Aequorea victoria and encodes a protein that absorbs blue light and emits green light. When a fly expressing GFP is illuminated by blue LEDs, filtered cameras can detect the green fluorescence that results and the fly's movement can be tracked at a rate of 60 frames per second. By linking the expression of GFP to the expression of other reporter genes, it is possible to determine when these genes are on or off, and how this is associated with a fly's behavior.

Tower said, "A large number of strains exist where GFP or some other auto-fluorescent protein is used as a reporter for specific gene expression in Drosophila and other organisms. -Our methods should be readily adaptable to such reagents, for example we have recently been successful in tracking DsRED fluorescent flies".


'/>"/>

Contact: Graeme Baldwin
graeme.baldwin@biomedcentral.com
44-079-205-86437
BioMed Central
Source:Eurekalert

Page: 1

Related biology technology :

1. New technique producing small things in large quantities
2. New DNA-based technique for assembly of nano- and micro-sized particles
3. NIH Announces Advanced Cell Technologys Single Cell Embryo Biopsy Technique as a Means to Derive Embryonic Stem Cells to be Considered for Federal Funding
4. New technique can be breakthrough for early cancer diagnosis
5. University of Leicester scientists discover technique to help friendly bacteria
6. Glycominds Joins Biomolecular Photonic (BMP) Consortium to Develop New Molecular Imaging Technique
7. Doping technique brings nanomechanical devices into the semiconductor world
8. Cardica and Intuitive Surgical to Host Educational Symposium on Advanced Techniques and Technologies in Robotic Coronary Revascularization and Mitral Valve Repair
9. DNA technique yields 3-D crystalline organization of nanoparticles
10. Unique infrared technique finds applications in nanoscience
11. Assembly technique for tiny wires may eventually help detect cancer and other diseases
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/28/2017)... WA (PRWEB) , ... March 28, 2017 , ... ... to announce the initial release of LabKey’s newest software solution, LabKey Biologics ... council of leading pharma and biotech organizations, LabKey Biologics provides drug research teams ...
(Date:3/28/2017)... ... March 28, 2017 , ... Currently symptomatic therapies for Parkinson’s ... study published in STEM CELLS suggests that human neural stem cell (hNSC) transplantation ... produce more neural cells. , Strategies involving transplantation of these cells into ...
(Date:3/28/2017)... NEW YORK , March 28, 2017 /PRNewswire/ ... a highly volatile and unpredictable sector due to ... space. Markets served include medical, agricultural, environmental, and ... these four stocks: Portola Pharmaceuticals Inc. (NASDAQ: PTLA), ... OCRX), and Ocular Therapeutix Inc. (NASDAQ: ...
(Date:3/28/2017)... (PRWEB) , ... March 28, ... ... to launch its brand-new, fully-certified hygienic SWB805 MultiMountTM weigh modules. These weigh ... guidelines and certified by National Sanitation Foundation (NSF). , As fully integrated ...
Breaking Biology Technology:
(Date:3/2/2017)... , March 2, 2017 Who risk to ... Download the full report: https://www.reportbuyer.com/product/4313699/ ... FINGERPRINT SENSOR FIELD? Fingerprint sensors using capacitive technology ... fingerprint sensor vendor Idex forecasts an increase of 360% ... devices and of the fingerprint sensor market between 2014 ...
(Date:3/1/2017)... , March 1, 2017  Aware, Inc. (NASDAQ: ... announced that Richard P. Moberg has resigned, ... co-President and Chief Financial Officer and Treasurer of Aware ... to serve as a member of the Board of ... , Aware,s co-Chief Executive Officer and co-President, General Counsel ...
(Date:2/28/2017)... DORTMUND, Germany , February 28, 2017 ... ... Amsterdam from 14 to 16 March, ... to destination, and show how seamless travel is a real benefit ... Materna has added biometrics to their passenger touch point solutions to ...
Breaking Biology News(10 mins):