Navigation Links
New mechanism for human gene expression discovered
Date:7/4/2013

channel proteins with the mutation still seemed to function normally.

Suspecting another factor at play, Gomez and his team instead focused on α1ACT, a poorly understood, free-floating fragment of the CACNA1A calcium channel protein known to express extra copies of glutamine in SCA6 cells. The researchers first looked at its origin and found that, to their surprise, α1ACT was generated from the same mRNA sequence as the CACNA1A calcium channel.

For the first time, they had evidence of a human gene that coded one strand of mRNA that coded two separate, structurally distinct proteins. This occurred due to the presence of a special sequence in the mRNA known as an internal ribosomal entry site (IRES). Normally found at the beginning of an mRNA sequence, this IRES site sat in the middle, creating a second location for ribosomes, the cellular machines that read mRNA, to begin the process of protein production.

Looking at function, Gomez and his team found that normal α1ACT acted as a transcription factor and enhanced the growth of specific brain cells. Importantly, mutated α1ACT appeared to be toxic to nerve cells in a petri dish, and caused SCA6-like symptoms in an animal model.

The team hopes to discover other examples of human genes with similar IRES sites to better understand the implications of this new class of "bifunctional" genes on our basic biology. For now, they are focused on leveraging their findings toward helping SCA6 patients and already are working on ways to silence mutated α1ACT.

"We discovered this genetic phenomenon in the pursuit of a disease cause and, in finding it, immediately have a potential strategy for developing preclinical tools to treat that disease," Gomez said. "If we can target the IRES and inhibit production of this mutant form of α1ACT in SCA6, we may be able to stop the progression of the disease."


'/>"/>

Contact: Kevin Jiang
kevin.jiang@uchospitals.edu
773-795-5227
University of Chicago Medical Center
Source:Eurekalert

Page: 1 2

Related biology technology :

1. UCSB researchers identify the mechanisms underlying salt-mediated behaviors in fruit flies
2. Understanding the molecular mechanisms underlying Alzheimers disease
3. Inflamax Research selected by the Immune Tolerance Network and the National Institutes of Health to conduct a landmark clinical study on the underlying mechanisms of allergic inflammation.
4. Penn research shows mechanism behind wear at the atomic scale
5. SomaGenics Reports Study on Mechanism of Action of Its Therapeutic Platform
6. Drexels Gogotsi and team advance understanding of energy storage mechanisms in Nature Materials
7. Nutra Pharma Announces Publication of a New Mechanism Study of Alpha-Cobratoxin in the Treatment of Pain
8. A New Mechanism for Tau Protein Pathology in Alzheimers Disease was Presented by Oligomerix, Inc. at the Society for Neuroscience Annual Meeting
9. Sorrento Therapeutics Presents Preclinical Data of its Fully Human Antibody Programs Targeting Anti-PD-L1 and Anti-PD-1 at the 8th Annual Next Generation Protein Therapeutics Summit
10. PROLOR Biotech To Present New Data On Its Long-Acting Human Growth Hormone And Long-Acting Oxyntomodulin At ENDO 2013
11. ChanTest Develops New Cardiotoxicity and Contractility Assay with Human Stem Cell Cardiomyocytes
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/30/2014)... 2014 BioHealth Innovation, Inc. (BHI), ... innovations and increasing access to early-stage funding in ... capitalist, Tania Fernandez , Ph.D., has joined the ... be a member of the management team for a ... $50 million in seed and early-stage equity investments to ...
(Date:9/30/2014)... iLab Solutions announced the results of ... which focuses on core facility operations, growth and utilization ... today. , In its 4th year running, a number ... the year-over-year decrease in the percentage of revenue that ... feeling the constraints of this trend. When asked, “What ...
(Date:9/30/2014)... The custom stainless steel fabrication company ... 2014. The Boston Area Chapter of ISPE (International Society ... event for Wed., Oct. 1 at Gillette Stadium Clubhouse ... largest in ISPE Boston’s long history, with more than ... expected. , HOLLOWAY AMERICA President David Simpson is ...
(Date:9/30/2014)... Boston, MA (PRWEB) September 30, 2014 ... communications (UC) and collaboration company is dedicated to ... data connectivity needs. Julie Power, a ... team to ensure collaboration solutions stays connected and ... response to the increased movement of businesses to ...
Breaking Biology Technology:Seasoned Life Sciences Venture Capitalist Tania Fernandez Joins BHI to Help Manage New BioHealth Gap Fund 2iLab Solutions Announces the Results of the 2014 Core Facility Benchmarking Study 2iLab Solutions Announces the Results of the 2014 Core Facility Benchmarking Study 3HOLLOWAY AMERICA to Exhibit at ISPE Boston Product Show 2014 2HOLLOWAY AMERICA to Exhibit at ISPE Boston Product Show 2014 3SoundConnect Expands Carrier Services Division 2
... Alliance, the leading,management consulting firm specializing in the ... has been appointed Chief,Operating Officer. His former role ... of the firm,s Brand Management Practice will be ... This new senior executive management role for Mr. ...
... Ondine Biopharma Corporation,(TSX: OBP; AIM: OBP), a ... that the results of a,study using the ... will be published in the peer-reviewed,journal Photochemistry ... e-published ahead of the print version and ...
... - GeneNews Limited (TSX: GEN), a,company focused on ... diseases and personalized health management, today reported,operational and ... to date,ended June 30, 2008., "The launch ... to assess a patient,s current risk of having ...
Cached Biology Technology:Nader Naeymi-Rad Appointed Chief Operating Officer at Campbell Alliance 2Nader Naeymi-Rad Appointed Chief Operating Officer at Campbell Alliance 3Ondine Announces Publication of Positive Results from Pseudomonas aeruginosa Eradication Study 2Ondine Announces Publication of Positive Results from Pseudomonas aeruginosa Eradication Study 3GeneNews Announces Second Quarter Results 2GeneNews Announces Second Quarter Results 3GeneNews Announces Second Quarter Results 4GeneNews Announces Second Quarter Results 5GeneNews Announces Second Quarter Results 6GeneNews Announces Second Quarter Results 7GeneNews Announces Second Quarter Results 8
(Date:9/29/2014)... CORVALLIS, Ore. Parents with children at home should ... from Oregon State University are recommending, after a new ... and asthma, asthma symptoms and chronic bronchitis. , "In ... the prevalence of asthma and wheezing is higher than ... ventilation," said Ellen Smit, an associate professor in the ...
(Date:9/29/2014)... beetles get a bad rap, and understandably so. The ... tens of millions of acres in the Western U.S. ... contrary to popular belief, these pests may not be ... have recently swept through the region. Instead, weather and ... of fires than these bark-boring beetles. , New research ...
(Date:9/29/2014)... Juan Manuel Santos has presented Dr. Cristin Samper, ... with the prestigious Order of San Carlos. , ... foreign civilians and military officers who have made ... field of international relations. , An international authority ... was invited to the Colombian Residence in New ...
Breaking Biology News(10 mins):Childhood asthma linked to lack of ventilation for gas stoves, OSU study shows 2Childhood asthma linked to lack of ventilation for gas stoves, OSU study shows 3Tree killers, yes, fire starters, no: Mountain pine beetles get a bad rap, study says 2Tree killers, yes, fire starters, no: Mountain pine beetles get a bad rap, study says 3Tree killers, yes, fire starters, no: Mountain pine beetles get a bad rap, study says 4
... holes in the limestone of the ocean floor using teeth ... their secrets, which might give engineers insights into creating ever-sharp ... fit their globular bodies using their five teeth, which, like ... continue to grow on the other end throughout the animals, ...
... Many proteins do not function by themselves as ... larger structures called protein complexes. The May issue ... ) features a set of methods that can be ... article details the generation of viral vectors for gene ...
... Fla. (May 4, 2009) The American Society for ... April 30 May 2 in Clearwater Beach, Florida, has ... to Howard J. Federoff, MD, PhD, Executive Vice President for ... at Georgetown University. , The Award is named for Bernard ...
Cached Biology News:True grit 2Protein analysis methods, viral vectors featured in Cold Spring Harbor Protocols 2Georgetown University's Howard J. Federoff, M.D., Ph.D. receives Bernard Sanberg Memorial Award 2
... - 5 units/µL ,• High specific activity ... Leaves an 'A' overhang ,• Processes ... very high activity in primer extension and ... quality of the enzyme, each lot has ...
Recombinant DNA polymerase from Thermus aquaticus expressed in E. coli; 5 units/uL; 25 tubes of 1, 000 units enzyme/tube w/ Buffer I...
... AmpliTaq Gold® DNA Polymerase is a ... When the chemical moiety is attached to ... set-up and the first ramp of thermal ... non-optimal annealing temperatures), the enzyme is inactive. ...
A robust formulation of Taq Polymerase supplied with a 10X colored reaction buffer which allows Direct Gel-Loading...
Biology Products: