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New laboratory method uses mass spectrometry to rapidly detect staph infections
Date:1/12/2012

Researchers from the Georgia Institute of Technology and the Centers for Disease Control and Prevention (CDC) have developed a new laboratory test that can rapidly identify the bacterium responsible for staph infections. This new test takes advantage of unique isotopic labeling combined with specific bacteriophage amplification to rapidly identify Staphylococcus aureus.

Quickly and accurately detecting infections caused by S. aureus is critical because the pathogenic bacterium causes a broad spectrum of infections, ranging from acute to chronic disease, which need to be treated in a prompt manner with the correct antibiotic.

The test uses mass spectrometry to quantify the number of S. aureus organisms in a large number of samples in just a few hours, compared to a day or two for culturing techniques typically used to detect this bacterium.

"Our method for detecting staph infections using mass spectrometry will be valuable in a variety of situations, but will be crucial when a large number of people need to be tested very quickly, which will ultimately improve treatment," said Facundo Fernndez, an associate professor in the Georgia Tech School of Chemistry and Biochemistry.

Details of the new staph infection detection method were published in the January issue of the journal Molecular and Cellular Proteomics. Partial funding for this research was provided by 3M and the CDC/Georgia Tech seed award program.

Fernndez together with Carrie Pierce, Jon Rees and John Barr from the CDC's Division of Laboratory Sciences created this test.

"The simplicity of sample preparation, the low cost of required reagents and the increased availability of mass spectrometers in clinical laboratories make this new method a cost-effective way to rapidly and effectively detect staph infections, which must be treated quickly to prevent spread of the disease," explained Pierce, a research chemist at the
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Contact: Abby Robinson
abby@innovate.gatech.edu
404-385-3364
Georgia Institute of Technology Research News
Source:Eurekalert  

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