Navigation Links
New label-free method tracks molecules and drugs in live cells
Date:12/18/2008

at the growing imbalance of saturated and unsaturated fats in our diets."

SRS microscopy could also prove useful in neuroimaging, since neurons are coated with fatty myelin sheaths.

The researchers' use of SRS microscopy to analyze skin tissue could also open new frontiers in drug development. Xie and colleagues used SRS microscopy to view how well retinoic acid, a topical acne medication, is absorbed into skin cells. They also used the technique to capture deep-skin penetration by dimethyl sulfoxide (DMSO), a compound added to many topical medications and ointments to enhance absorption.

Scientists currently use a variety of techniques to visualize biomolecules, but most have significant limitations that are sidestepped by SRS microscopy. A jellyfish protein first discovered in 1962, green fluorescent protein (GFP), is now used extensively as a label for observing the activity of biomolecules. GFP labeling provides sharp images, but the bulky protein can perturb delicate biological pathways, especially in cases where its heft overwhelms smaller biomolecules. Also, GFP's characteristic glow subsides with time, making it infeasible for long-term tracking.

Much like SRS microscopy, conventional infrared (IR) and Raman microscopies measure the vibrations of chemical bonds between atoms. But they are low-sensitivity imaging techniques, and require either desiccated samples or high laser power, which limits use in imaging live specimens. Coherent anti-Stokes Raman scattering (CARS) microscopy, a field pioneered by Xie's own group, cannot provide clear enough contrast for most molecules.


'/>"/>

Contact: Steve Bradt
steve_bradt@harvard.edu
617-496-8070
Harvard University
Source:Eurekalert

Page: 1 2

Related biology technology :

1. Pitt researchers create nontoxic clean-up method for potentially toxic nano materials
2. Parion Sciences Receives NIH Biodefense Grant to Study Novel Methods for Mitigating Radiation-Induced Lung Injury
3. Nikon Instruments Introduces Exclusive VAAS Detector Imaging Method for Confocal Microscopy
4. Method sorts out double-walled carbon nanotube problem
5. Stem Cell Sciences Signs Service Agreement With Pfizer to Provide Access to Proprietary Technologies and Methods
6. Researchers discover method for mass production of nanomaterial graphene
7. Stretching silicon: A new method to measure how strain affects semiconductors
8. Waters Publishes Rapid and Specific Method of Detection for Melamine in Infant Formula and Liquid Milk, in Support of Chinas Ministry of Science and Technology
9. SpectraScience Awarded Patent for Its Optical Biopsy System and Methods for Tissue Diagnosis
10. UC Riverside researcher develops novel method to grow human embryonic stem cells
11. PerkinElmer Expertise and Solutions Integral to Food Safety Monitoring for the Beijing Games : Methods, Applications Expertise and Instrumentation Deployed in Mobile Laboratory
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/28/2014)... July 28, 2014 Appistry, Inc. ... that bring the power of genomics to next-generation medicine, ... Appistry Pipeline Challenge . , Two Appistry staff ... the National Center for Genome Resources, and Mr. Neil ... Clinics of Kansas City. Rounding out the panel are ...
(Date:7/28/2014)... -- Research and Markets has announced the addition ... report to their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... used to determine the size of a particle in a ... analyze the size of particles as it can affect the ... to determine the behavior and nature of a material, and ...
(Date:7/25/2014)... Trillions of bacteria live in each person,s digestive tract. ... food and stave off harmful infections, but their role ... help shed light on the role of these bacteria, ... Eric Alm recently tracked fluctuations in the bacterial populations ... findings, described in the July 25 issue of the ...
(Date:7/25/2014)... The Conferee Networking committee is pleased to ... These two-hour sessions provide a unique networking opportunity for ... discuss new techniques, or brainstorm new ideas in an ... for consideration is August 31, 2014, and instructions can ... committee will review the topics, and status notifications will ...
Breaking Biology Technology:Appistry Names Industry Experts from the National Center for Genome Resources and Mercy Children's, Kansas City, to Judge Pipeline Challenge Competition 2Global Particle Size Analysis Market 2014-2018 2Monitoring the rise and fall of the microbiome 2Monitoring the rise and fall of the microbiome 3Pittcon 2015 Announces Call for Topics for Conferee Networking Sessions 2
... from McGill,s Physics Department and Mike Lilly from Sandia ... electronic circuits. It is formed by two wires separated ... This discovery, published in the journal Nature Nanotechnology ... and power of the ever smaller integrated circuits of ...
... LONDON, December 7, 2011 Silence Therapeutics plc ... ( RNAi ) therapeutics company, announces a positive outcome from the ... (EPO) over opposition to Silence,s granted European Patent EP 1 536 ... The granted patent refers to the gene target PKN3, protein ...
... 2011  PDL BioPharma, Inc. (PDL) (NASDAQ: PDLI ) ... , 2.875% Convertible Senior Notes due February 15, 2015 ... $1,000 principal amount or approximately $6.44 per share, effective December ... May 1, 2015 (May 2015 Notes) is 135.9849 shares of ...
Cached Biology Technology:Silence Therapeutics Announces Positive Outcome From Oral Hearing at the European Patent Office 2Silence Therapeutics Announces Positive Outcome From Oral Hearing at the European Patent Office 3Silence Therapeutics Announces Positive Outcome From Oral Hearing at the European Patent Office 4PDL BioPharma Announces Adjusted Conversion Rates for Its Convertible Notes 2
(Date:7/27/2014)... study has revealed that nicotine and cotinine, a metabolite ... a certain carcinogen in smoke. , The carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone ... leaves and ultimately ends up in the tobacco smoke. ... liver, where it is activated by a variety of ... has revealed that nicotine can partially interfere with the ...
(Date:7/27/2014)... the southern Western Ghats, a mountain range along the west ... that any mayfly belonging to the genus Labiobaetis ... called Labiobaetis soldani , "is named in honor of ... of the Ephemeroptera of Palaearctic and Oriental realms," according to ... in the Journal of Insect Science . , The ...
(Date:7/27/2014)... at Barts Cancer Institute have found that targeting a ... more effective, according to research published in Nature ... part of Queen Mary University of London, have found ... the body to repair itself after chemotherapy or radiotherapy, ... researchers removed FAK from blood vessels that grew in ...
Breaking Biology News(10 mins):Nicotine found to inhibit DNA-strand break caused by a certain carcinogen in smoke 2New drug target can break down cancer's barrier against treatment 2
... antibacterial medicine that kills the superbug MRSA is being ... Trust. The Seeding Drug Discovery initiative is aimed at ... unmet need. , Prolysis, an R&D company based ... Drug Discovery awards for its new antibacterial compounds aimed ...
... discovery in stem cell research may mean big things for ... a research team at the University of Kentucky published their ... , The researchers genetically mapped a stem cell gene and ... the investigation all the way through to the identification of ...
... as SORL1 may be a factor in the development of ... discovered. The genetic clue, which could lead to a better ... Genetics online, Jan. 14, 2007, and was supported in part ... researchers suggest that faulty versions of the SORL1 gene contribute ...
Cached Biology News:New antibacterials being developed to tackle MRSA superbug 2Researchers first to map gene that regulates adult stem cell growth 2Scientists find new genetic clue to cause of Alzheimer's disease 2
5-bromo-4-chloro-3-indolyl b -D-galactopyranoside (X-Gal)...
... Attoglow Western Blot Analysis Kit is ... kit developed by Biochain. It detects ... g) or yoctomole (10 -24 ... three parts: Millennium Enhancer, protein interaction ...
... BioNumerics is the universal bioinformatics software that ... data. The unique modular design of BioNumerics ... virtually any type of data employed for ... microarrays, DNA fingerprints, 2-D protein gels, HPLC, ...
... the early 80s Biosearch Technologies has ... oligonucleotide tools to accelerate the discovery ... you need dual-labeled FRET probes and ... oligos for microarray applications, Biosearch can ...
Biology Products: