Navigation Links
New label-free method tracks molecules and drugs in live cells
Date:12/18/2008

at the growing imbalance of saturated and unsaturated fats in our diets."

SRS microscopy could also prove useful in neuroimaging, since neurons are coated with fatty myelin sheaths.

The researchers' use of SRS microscopy to analyze skin tissue could also open new frontiers in drug development. Xie and colleagues used SRS microscopy to view how well retinoic acid, a topical acne medication, is absorbed into skin cells. They also used the technique to capture deep-skin penetration by dimethyl sulfoxide (DMSO), a compound added to many topical medications and ointments to enhance absorption.

Scientists currently use a variety of techniques to visualize biomolecules, but most have significant limitations that are sidestepped by SRS microscopy. A jellyfish protein first discovered in 1962, green fluorescent protein (GFP), is now used extensively as a label for observing the activity of biomolecules. GFP labeling provides sharp images, but the bulky protein can perturb delicate biological pathways, especially in cases where its heft overwhelms smaller biomolecules. Also, GFP's characteristic glow subsides with time, making it infeasible for long-term tracking.

Much like SRS microscopy, conventional infrared (IR) and Raman microscopies measure the vibrations of chemical bonds between atoms. But they are low-sensitivity imaging techniques, and require either desiccated samples or high laser power, which limits use in imaging live specimens. Coherent anti-Stokes Raman scattering (CARS) microscopy, a field pioneered by Xie's own group, cannot provide clear enough contrast for most molecules.


'/>"/>

Contact: Steve Bradt
steve_bradt@harvard.edu
617-496-8070
Harvard University
Source:Eurekalert

Page: 1 2

Related biology technology :

1. Pitt researchers create nontoxic clean-up method for potentially toxic nano materials
2. Parion Sciences Receives NIH Biodefense Grant to Study Novel Methods for Mitigating Radiation-Induced Lung Injury
3. Nikon Instruments Introduces Exclusive VAAS Detector Imaging Method for Confocal Microscopy
4. Method sorts out double-walled carbon nanotube problem
5. Stem Cell Sciences Signs Service Agreement With Pfizer to Provide Access to Proprietary Technologies and Methods
6. Researchers discover method for mass production of nanomaterial graphene
7. Stretching silicon: A new method to measure how strain affects semiconductors
8. Waters Publishes Rapid and Specific Method of Detection for Melamine in Infant Formula and Liquid Milk, in Support of Chinas Ministry of Science and Technology
9. SpectraScience Awarded Patent for Its Optical Biopsy System and Methods for Tissue Diagnosis
10. UC Riverside researcher develops novel method to grow human embryonic stem cells
11. PerkinElmer Expertise and Solutions Integral to Food Safety Monitoring for the Beijing Games : Methods, Applications Expertise and Instrumentation Deployed in Mobile Laboratory
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/23/2014)... Cambridge Semantics, the leading provider of ... named in the recent ‘Hype Cycle for Life Sciences ... report details key insights pertaining to the benefits in ... analysts Michael Shanler and Stephen Davies, “The use of ... relationships with scientific stakeholders, and support collaboration and innovation ...
(Date:9/23/2014)... 23, 2014 Marktech Optoelectronics has introduced ... violet emitters in the 280 nm wavelength range. This ... which range from 280nm to 405nm. These new devices ... as well as curing. Mark Campito, Marktech’s CEO states ... interest to manufacturers is that we offer it in ...
(Date:9/23/2014)... 23, 2014 New spin-out ... Innovations, Cambridge Innovation Capital and Johnson & Johnson ... genomics company focused on harnessing the potential of ... testing and treatment, today announced it has raised ... Innovations and including Cambridge Innovation Capital and Johnson ...
(Date:9/22/2014)... 2014 Research and Markets ... the "Global Biotechnology Reagents Market 2014-2018" ... http://photos.prnewswire.com/prnh/20130307/600769) Biotechnology reagents are chemical compounds ... to detect and examine another substance to provide ... laboratories to prepare, collect, and examine specimens from ...
Breaking Biology Technology:Cambridge Semantics Identified in Gartner’s ‘Hype Cycle for Life Sciences 2014’ Report 2Marktech Introduces its New Line of Deep UV Emitters 2Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 2Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 3Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 4Global Biotechnology Reagents Market 2014-2018: Key Vendors are Agilent, BD Biosciences, Bio-Rad, F. Hoffmann-La Roche, Sigma-Aldrich and Thermo Fisher 2Global Biotechnology Reagents Market 2014-2018: Key Vendors are Agilent, BD Biosciences, Bio-Rad, F. Hoffmann-La Roche, Sigma-Aldrich and Thermo Fisher 3
... via Single ... Platform, MirixaPro(SM), RESTON, Va. and WASHINGTON, Oct. ... creator of,the Diabetes Ten City Challenge and a partner in the ... that will,deliver all of APhA Foundation,s HealthMapRx commercial programs via,Mirixa. MirixaPro(SM) ...
... Oct. 9 eResearchTechnology,Inc. (eRT), (Nasdaq: ERES ... technology, ePRO and other services to the pharmaceutical,biotechnology, ... will release its 2008 third quarter results for ... 30, 2008 after the market closes.,After the release, ...
... CEL-SCI Corporation,(NYSE: CVM ) announced today ... facility for its lead drug Multikine(R). This dedicated,facility, ... Multikine that,will be used for CEL-SCI,s pivotal Phase ... head and neck cancer patients, and,subsequently for sale ...
Cached Biology Technology:Mirixa Corporation and the APhA Foundation Form Partnership 2Mirixa Corporation and the APhA Foundation Form Partnership 3eResearchTechnology, Inc. to Announce 2008 Third Quarter Results on October 30, 2008 2CEL-SCI Takes Delivery of New Manufacturing Facility 2
(Date:9/22/2014)... 2011 the Society of Environmental Toxicology and Chemistry (SETAC) ... and prioritize the scientific research needed to understand the ... the environment. The effort was extended, and results ... Society,s international journal, Integrated Environmental Assessment and Management ... a podcast interviewing the lead author of the study, ...
(Date:9/22/2014)... Additive manufacturing (AM), or 3D printing, has rapidly ... metal components strong enough for structural applications. However, ... as well as quality standards to test the ... technology. Engineers at the University of Pittsburgh,s Swanson ... modeling and simulation (M&S) technology and new qualification ...
(Date:9/22/2014)... and larger areas of sea ice melt in the summer ... it is thinner and more reduced. As the Arctic summers ... warming, because reduced sea ice in the Arctic will remove ... our results are representative, then sea ice plays a greater ... in future global CO2 budgets", says Dorte Haubjerg Sgaard, PhD ...
Breaking Biology News(10 mins):Priorities for research on pharmaceutical and personal care products in the environment 2Pitt engineers receive grants to enhance additive manufacturing 2Pitt engineers receive grants to enhance additive manufacturing 3Arctic sea ice helps remove CO2 from the atmosphere 2
... Nov. 21, 2011 Already partnering with 43 of ... Top 100 Innovators, NineSigma today announced a record year ... is critical to their long-term growth. "The ... never hesitate in their quest to innovate," said Andy ...
... data from electronic tags, ear bone microchemistry and fisheries catch ... of the Atlantic influences the other side. Bluefin populations ... since 1950, according to the study published today in the ... estimates the number of Atlantic bluefin tuna remaining in the ...
... FLAGSTAFF, Ariz. More peer-reviewed scientific studies ... developments and operations are needed to adequately assess their ... scientific literature review conducted by the U.S. Geological Survey ... In their literature review, the authors of the paper, ...
Cached Biology News:Surging Demand for Innovation Services Leads to Record Expansion for NineSigma 2Surging Demand for Innovation Services Leads to Record Expansion for NineSigma 3New study provides comprehensive view of the status of Atlantic bluefin tuna 2New study provides comprehensive view of the status of Atlantic bluefin tuna 3Literature review on solar energy and wildlife impacts research 2
MHC Volumes 1 & 2...
Hucal Agx Antigen Expression Service Specificity: Hucal Agx ...
MitoScreen (JC-1) Kit , 100 tests Consult technical datasheet for details....
Large-fragment, single-stranded carrier DNA of the highest quality, perfectly suited for all types of yeast transformation....
Biology Products: