Navigation Links
New force-fluorescence device measures motion previously undetectable
Date:10/11/2007

. Therefore, by monitoring the brightness of the two dyes, the researchers can determine the motion of the molecule.

The optical trap, on the other hand, functions somewhat like the fictional tractor beam in Star Trek. In this case, a focused laser beam locks onto a microsphere attached to one end of the molecule to be studied. The optical trap can then pull on the molecule like a pair of tweezers.

By combining the two techniques, we get the best of both worlds, said Ha, who also is an affiliate of the universitys Institute for Genomic Biology and of the Howard Hughes Medical Institute. Using the optical trap, we can pull on DNA strands with forces as small as half a pico-newton. Using single-molecule fluorescence resonance energy transfer, we can measure the resulting conformational changes with nanometer precision.

By probing the dynamics of the Holliday junction in response to pulling forces in three different directions, the researchers mapped the location of the transition states and deduced the structure of the transient species present during the conformational changes.

Based on our previous studies, we knew the Holliday junction fluctuated between two structures, Ha said, but how it moved from one place to the other, and what intermediates were visited along the pathway, were unknown.

With this latest work, the researchers have deduced the pathway of the conformational flipping of the Holliday junction, and determined the intermediate structure is similar to that of a Holliday junction bound to its own processing enzyme.

The next challenge is to obtain a timeline of movement by force, for example, due to the action of DNA processing enzymes, and correlate it with the enzyme conformational changes simultaneously measured by fluorescence, Ha said.

With Ha, co-authors of the paper are former U. of I. postdoctoral research associate and lead-author Sungchul Hohng (now at Seoul National University); physics professor Klaus Schulten; gr
'/>"/>

Contact: James E. Kloeppel, Physical Sciences Editor
kloeppel@uiuc.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Page: 1 2 3

Related biology technology :

1. Comparison of Performance Characteristics of Different Biolistic Devices
2. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
3. TomoTherapy to sell medical devices in India
4. Security concerns grow with mobile tech devices
5. Am I spoiled if I expect well-designed mobile devices?
6. Midwest retains dominant role in 2005 world medical device market
7. Students will show off biomedical devices to professors and investors
8. RFID medical devices - Opportunities and challenges
9. State of biotech, medical device sector is focus of upcoming conference
10. Midwest plays key role in developing new medical device companies
11. Medical device industry growing in importance in Midwest
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/2/2015)... 2, 2015  RXi Pharmaceuticals Corporation (NASDAQ: ... on discovering and developing innovative therapies primarily ... today announced that Dr. Pamela Pavco , ... the 23 rd World Congress of ... include in-depth presentations by world-class clinicians and/or ...
(Date:6/2/2015)... 2, 2015  Alere Inc. (NYSE: ALR ... the White House Antibiotic Stewardship Forum today to ... and advance the President,s National Action Plan to ... number of measurable goals and objectives over the ... healthcare providers protect their patients through antibiotic stewardship, ...
(Date:6/2/2015)... Through rare discovery of new physics ... is enabling World Scientists to actually see, watch, ... displaying the full nature (position, shape, size, number) of ... Porter, Co-Founder of the Yancy Corporation, says, "Our rare ... They come once in a lifetime, if at all. ...
(Date:6/2/2015)... Sunnyvale, CA (PRWEB) June 02, 2015 ... their official United States distributor for the Moenninghoff ... Finger stated, “I am pleased to announce that the ... U.S. market. For our firm, founded in 1916, this ... that R.M. Hoffman is the kind of professional distributor ...
Breaking Biology Technology:RXi Pharmaceuticals to Present at the 23rd World Congress of Dermatology 2RXi Pharmaceuticals to Present at the 23rd World Congress of Dermatology 3RXi Pharmaceuticals to Present at the 23rd World Congress of Dermatology 4Alere Announces Broad Initiatives to Help Combat Antibiotic Resistance during White House Antibiotic Stewardship Forum 2Scientists Eying Rare Discovery Of New Physics & Light Microscope Technology 2R.M. Hoffman Company designated official United States distributor for the Moenninghoff product line. 2R.M. Hoffman Company designated official United States distributor for the Moenninghoff product line. 3
... CITY, Oct. 28 /PRNewswire-FirstCall/ - AEterna Zentaris Inc.,(NASDAQ: ... company focused on,endocrine therapy and oncology, today announced ... Paul Blake, MD, will present a company,overview at ... November 3, 2008 at 8:00 am (Eastern Time), ...
... at the ... conference, booth 519, ... development of cost-effective, easy-to-use gene-expression analysis,systems, announced that renowned breast ... in the Department of,Biochemistry and Molecular Biology at the University ...
... bones of mammals, the protective shells of mollusks, and the ... made-from-scratch wonders of nature. , Used to crush food, for ... teeth and bones are composed are the strongest and most ... long sought to mimic them. , Now, harnessing the process ...
Cached Biology Technology:James L. Wittliff, Ph.D., FACB, to Present 'Predicting Breast Cancer Outcome with Gene Expression Signatures on the Ziplex(R) System' at the Association for Molecular Pathology Conference, October 30 2James L. Wittliff, Ph.D., FACB, to Present 'Predicting Breast Cancer Outcome with Gene Expression Signatures on the Ziplex(R) System' at the Association for Molecular Pathology Conference, October 30 3Sea urchin yields a key secret of biomineralization 2Sea urchin yields a key secret of biomineralization 3
(Date:4/27/2015)... Profile Solutions, Inc. (OTC: PSIQ), a leading ... pleased to announce that Dr Gerry Bedore ... of its scientific advisory board. ... leader in technology-enhanced learning models. He was a co-founder ... studies and books focused on online student success and ...
(Date:4/20/2015)... , April 20, 2015 Huntington Memorial Hospital ... to implant a new miniaturized, wireless monitoring sensor to ... the first and only FDA-approved heart failure monitoring device ... when used by physicians to manage heart failure. ... is implanted in the pulmonary artery (PA) during a ...
(Date:4/17/2015)... BURNABY, Canada , April 17, 2015 /PRNewswire/ ... projects, growing security concerns, and technological advancement to ... Arabia until 2020   ... " Saudi Arabia Biometric Systems Market Forecast &  Opportunities, 2020 ", biometric ... is projected to register growth at CAGR ...
Breaking Biology News(10 mins):Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 2Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 3Profile Solutions Names Medicinal Cannabis, Agriculture and Technology Expert Dr. Gerry Bedore to its Scientific Advisory Board 4Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3
... Nearly 50% of couples who were interviewed at least 3 ... their surplus, stored embryos for stem cell research, according to ... April 26, 2007, advanced online publication of Cell Stem Cell, ... International Society for Stem Cell Research. , The key ...
... MIT Media Lab have invented a way to reversibly ... the prospect of controlling the haywire neuron activity that ... , Such diseases often must be treated by removing ... lead to the development of optical brain prosthetics to ...
... Dick at Ontario Cancer Institute (OCI) have developed a method ... stem cells. The converted cells, when transplanted into special mice ... entire disease process from the very moment it begins. The ... Dick, Senior Scientist at OCI, the research arm of Princess ...
Cached Biology News:Many couples choose to donate surplus embryos for stem cell research 2MIT: Pulsing light silences overactive neurons 2Cancer scientists create 'human' leukemia process to map how disease begins, progresses 2
Agarose-ME, 500 g. Suitable for immunoelectrophoresis.Gel point (1.5%): 36 1.5 C, EEO (-mr): 0.16-0.19, Gel strength (1%): > 1000 g/cm2. Category: Nucleotides & Enzymes & Biochemicals, Ultrapure Bio...
... while maintaining high cell viability , ... , Fast: less than one ... , Highly reproducible results from ... with the Bio-Rad Gene Pulser Xcell System ...
100 adhesive strips....
Request Info...
Biology Products: