Navigation Links
New force-fluorescence device measures motion previously undetectable
Date:10/11/2007

. Therefore, by monitoring the brightness of the two dyes, the researchers can determine the motion of the molecule.

The optical trap, on the other hand, functions somewhat like the fictional tractor beam in Star Trek. In this case, a focused laser beam locks onto a microsphere attached to one end of the molecule to be studied. The optical trap can then pull on the molecule like a pair of tweezers.

By combining the two techniques, we get the best of both worlds, said Ha, who also is an affiliate of the universitys Institute for Genomic Biology and of the Howard Hughes Medical Institute. Using the optical trap, we can pull on DNA strands with forces as small as half a pico-newton. Using single-molecule fluorescence resonance energy transfer, we can measure the resulting conformational changes with nanometer precision.

By probing the dynamics of the Holliday junction in response to pulling forces in three different directions, the researchers mapped the location of the transition states and deduced the structure of the transient species present during the conformational changes.

Based on our previous studies, we knew the Holliday junction fluctuated between two structures, Ha said, but how it moved from one place to the other, and what intermediates were visited along the pathway, were unknown.

With this latest work, the researchers have deduced the pathway of the conformational flipping of the Holliday junction, and determined the intermediate structure is similar to that of a Holliday junction bound to its own processing enzyme.

The next challenge is to obtain a timeline of movement by force, for example, due to the action of DNA processing enzymes, and correlate it with the enzyme conformational changes simultaneously measured by fluorescence, Ha said.

With Ha, co-authors of the paper are former U. of I. postdoctoral research associate and lead-author Sungchul Hohng (now at Seoul National University); physics professor Klaus Schulten; gr
'/>"/>

Contact: James E. Kloeppel, Physical Sciences Editor
kloeppel@uiuc.edu
217-244-1073
University of Illinois at Urbana-Champaign
Source:Eurekalert

Page: 1 2 3

Related biology technology :

1. Comparison of Performance Characteristics of Different Biolistic Devices
2. Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
3. TomoTherapy to sell medical devices in India
4. Security concerns grow with mobile tech devices
5. Am I spoiled if I expect well-designed mobile devices?
6. Midwest retains dominant role in 2005 world medical device market
7. Students will show off biomedical devices to professors and investors
8. RFID medical devices - Opportunities and challenges
9. State of biotech, medical device sector is focus of upcoming conference
10. Midwest plays key role in developing new medical device companies
11. Medical device industry growing in importance in Midwest
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/29/2014)... of the most promising technologies for future quantum circuits ... instead of electrons. First, it is necessary to be ... control their direction. Researchers around the world have made ... now scientists at the Niels Bohr Institute have succeeded ... at a time and in a particular direction. The ...
(Date:8/29/2014)... Pittcon is pleased to announce that ... Matsuda, Horiba Scientific, have organized a session on nanotechnology ... JASIS , Asia’s largest analytical and scientific instruments show, ... Japan. , The four speakers in this U.S. ... Molecules to Single Cells” will present their use of ...
(Date:8/29/2014)... Three Lawrence Livermore researchers have received ... R&D Award for their research in producing ... , Shared with collaborators from the National Renewable ... Las Vegas (UNLV), the award recognizes the team ... production and corrosion processes. , These models have ...
(Date:8/29/2014)... Antonio, TX (PRWEB) August 29, 2014 ... and ISO 13485:2003 certified, GAMP® 5 compliant imaging ... awarded an international Phase II clinical trial to ... lymphoma. , Throughout this trial, Intrinsic Imaging will ... not limited to, protocol and charter development, site ...
Breaking Biology Technology:Breakthrough in light sources for new quantum technology 2Fifth Annual U.S. Symposium at JASIS Organized by Pittcon’s Program Chairman Hub MacDonald 2Livermore Team Awarded for Hydrogen Production Research 2Intrinsic Imaging Awarded Phase II Clinical Trial to Assess New Treatment for Non-Hodgkin’s Lymphoma 2
... , , Multiporator / Electroporator 2510 , , , , , , ... Transformation Protocol , Protocol No. 4308 915.541 03/2002 , , ... , , , Microorganism , ... Cell type , Bacteria, gram positive, ...
... , , , , , , , , ... Protocol No. 4308 915.533 01/2002 , , , , , ... , Microorganism , Staphylococcus aureus, ... Bacteria, gram positive, , , ...
... Multiporator / Electroporator 2510 , , , , , , , ... Protocol No. 4308 915.532 01/2002 , , , ... , Microorganism , ... Cell type , Bacteria, gram negative, , ...
Cached Biology Technology:Streptococcus salivarius 2Staphylococcus aureus 2Salmonella typhimurium 2
(Date:8/28/2014)... have identified a handful of bacterial culprits that may ... and ulcerative colitis, using patients, own intestinal immune responses ... 28 in the journal Cell . , Trillions ... plays a critical role in the development and progression ... number of bacterial species affect a person,s susceptibility to ...
(Date:8/28/2014)... Clinic researcher and his collaborators have developed an online ... process of re-engineering cells for biomedical investigation. CellNet is ... to aid stem cell engineering. Details of CellNet and ... two back-to-back papers in the journal Cell . ... uses for all types of cell-based investigations and can ...
(Date:8/28/2014)... 28, 2014  Privacy Advocate and Senior Staff Attorney ... , joins the lineup of biometric and mobile commerce ... UnPlugged Executive Summit in Tampa, Florida ... announced speakers include Steven Rahman, Director, Technology and Strategy at ... Experian. The theme of this year,s event is Mobility ...
Breaking Biology News(10 mins):Yale study identifies possible bacterial drivers of IBD 2New tool aids stem cell engineering for medical research 2Biometrics UnPlugged Welcomes the Electronic Frontier Foundation's Jennifer Lynch to the Mobility at the Crossroads of Commerce and Privacy Summit 2
... Montreal researchers has discovered that GCN2, a protein in ... long-term memory, may be a master regulator of the ... control of hippocampal synaptic plasticity and memory by the ... 25th issue of the journal Nature, provides the first ...
... motors are nanoscale engines which move along very thin rod-like ... molecular cargo within biological cells. Both motors and filaments can ... transport systems. In order to increase the flux of the ... of motors that contribute to this transport but, at the ...
... ingenious mechanism used by a particular enzyme to modify ... to designing a new kind of drug that could ... will be published in the May 6 issue of ... years that an enzyme called Dam (DNA adenine methyltransferase) ...
Cached Biology News:Montreal researchers probe the genetic basis of memory 2Motor transport in bio-nano systems 2Motor transport in bio-nano systems 3Motor transport in bio-nano systems 4Scientists take aim at virulent bacteria by decoding machinery of key control enzyme 2
... of an anodized aluminum body in a ... embedded in polymerized matrix. It is designed ... Synergy Multi-Detection Readers. This multi-functional test plate ... the need for tedious wet testing. Works ...
... The DMW143 taken from the SMZ line ... environments of production and testing. It is available ... base (FBGG) or a space-saving compact base (N2GG). ... computer, TV and a projector all at the ...
... siRNA applications, contains magnetic nanoparticles (MagTag) which ... of interest. Exploiting magnetic force the full ... towards and delivered into the target cells ... be used for adherent cells; for suspension ...
...
Biology Products: