Navigation Links
New findings could overcome major stumbling blocks to tissue cryopreservation for medical care
Date:11/5/2013

Developing an efficient way to freeze and store living tissues could transform many aspects of medical care and research, but ice crystallization often occurs within cells during such cryopreservation procedures, leading to cell death. In the November 5 issue of the Biophysical Journal, a Cell Press publication, researchers report that they have gained new information about the processes that are responsible for promoting the freezing of cells within tissues. This knowledge may ultimately lead to novel approaches for preventing tissue injury during cryopreservation.

A long-standing obstacle to avoiding tissue damage during freezing is that when cells are joined together within tissues, individual cells are more likely to crystallize than if the cells are kept apart. "In tissues, ice crystals are thought to be able to grow through membrane channels called gap junctions, thus allowing ice to easily propagate from cell to cell," explains senior author Dr. Jens Karlsson, of the Department of Mechanical Engineering at Villanova University. "But the results of the present study indicate that the mechanism of tissue cryo-injury is much more complex than was previously thought."

Dr. Karlsson and his team monitored microscopic freezing events inside genetically modified cells and used mathematical models to show that gap junctions do not always provide the major pathway for the spreading of ice crystals between cells. They saw that cell-to-cell propagation of ice also occurred during freezing of tissue samples in which gap junction formation had been suppressed. The authors discovered that intercellular connectionsin which neighboring cell membranes are stitched together by rows of rivet-like structures known as tight junctionsalso play a significant role.

"By using high-speed video imaging, we found evidence that ice from outside the cells sometimes forms nanoscale branches, which can penetrate the barriers created by tight junction seams," says Dr. Karlsson. "The resulting invasion of the spaces between cells appears to promote crystallization of the cells adjacent to the breach."

The unexpected findings may provide a boon for the manufacturing of engineered tissue products that can be used for grafts and organ transplantations. "Using cryopreservation to stop living tissue constructs from spoiling during storage will be the key to enabling economical mass production, quality assurance, and shipping logistics for these life-saving products," says Dr. Karlsson.


'/>"/>

Contact: Mary Beth O'Leary
moleary@cell.com
617-397-2802
Cell Press
Source:Eurekalert  

Related biology technology :

1. US Oncology Research Affiliated Physician to Present Findings From Innovative Clinical Trial at 2011 CTRC-AACR San Antonio Breast Cancer Symposium
2. 23andMe Presents Top Ten Most Interesting Genetic Findings of 2011
3. Live Press Briefing on Findings of Biomedical Industry CEO Survey
4. GeoVax Labs Presents New Findings on Potential HIV Vaccine at 2012 Conference on Retroviruses and Opportunistic Infections (CROI)
5. Expanded Findings for FirstMarks Completed Clinical Study for Predicting Near-Term (2-3 Years) MI
6. Profil Institute Continues to Publish New Findings in Metabolic Research
7. New Findings of Daily Consumption of Soy Isoflavones and Cacao-Flavonoids in Diabetes Type 2 Patients
8. Published Findings In Human Gene Therapy Methods Journal Demonstrate Cardiums New Catheter-Based Method Significantly Boosts Gene Delivery To The Heart
9. Findings could be used to engineer organs
10. ENDECE Neural to Present Findings at 2013 Biotech Showcase on NDC-1308 which Induces Remyelination of Demyelinated Axons
11. Fertility Associates of Memphis, World-Renowned Expert in Infertility, Presents Findings to European Conference
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
New findings could overcome major stumbling blocks to tissue cryopreservation for medical care
(Date:2/11/2016)...  Wellcentive today announced it has been selected ... -based community care organization (CCO) with more than ... reporting and care management solutions and services. Wellcentive,s ... quality managers, analysts and care managers while providing ... FamilyCare members. Oregon ...
(Date:2/11/2016)... , Feb. 11, 2016   BioInformant announces ... "Stem Cell Research Products, Opportunities, Tools, and Technologies – ... The ... the stem cell industry, BioInformant has more than a ... stem cell market, by stem cell type. This powerful ...
(Date:2/10/2016)... , Febr. 10, 2016 /PRNewswire/ - BioAmber Inc. (NYSE: ... to announce that Mitsui & Co. Ltd., its partner ... acid plant, is investing an additional CDN$25 million in ... its stake from 30% to 40%.  Mitsui will also ... acid produced in Sarnia , providing ...
(Date:2/10/2016)... 2016 ASAE is introducing a hybrid membership ... (AMC) the option of joining or renewing through an ... by staff size, every employee in any size association ... reap all available member benefits.   John ... membership options will allow organizations of any size and ...
Breaking Biology Technology:
(Date:2/1/2016)... 1, 2016  Today, the first day of American ... to develop a first of its kind workplace health ... Watson. In the first application of Watson ... IBM ), and Welltok will create a new offering ... cognitive analytics, delivered on Welltok,s health optimization platform. The ...
(Date:1/28/2016)... Jan. 28, 2016 Synaptics (NASDAQ: SYNA ), a ... its second quarter ended December 31, 2015. ... of fiscal 2016 increased 2 percent compared to the comparable quarter ... of fiscal 2016 was $35.0 million, or $0.93 per diluted share. ... income for the first quarter of fiscal 2016 grew 9 percent ...
(Date:1/25/2016)... Unisys Corporation (NYSE: UIS ) today announced the implementation ... New York City , to help U.S. ... the United States using passports that are ... of the system at Dulles last year. The system will ... 2016. --> pilot testing of the system at ...
Breaking Biology News(10 mins):