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New Research on Rapid Identification and Strain Typing of Salmonella in Food to Be Presented at IAFP
Date:7/18/2013

Assay" onclick="linkClick(this.href)">Salmonella serovars frequently involved in food safety and public health outbreaks, and to provide identification in less than five hours to aid in epidemiological investigation and source tracking. This study used a collection of more than 230 Salmonella strains provided by the Food and Drug Administration (FDA), and representing the most frequently encountered Salmonella serovars associated with human illness or isolated from food products. Strains clustered into serovar-specific branches on the GSS phylogenetic tree, with multi-lineage serovars like Newport and Saintpaul forming more than one distinctly separated branch.

The second poster demonstrates the use of the RESOLUTION System to accurately determine pathogen subtype and serotype information in five hours, and to derive additional strain and serotype information directly from contaminated foods enriched in commercial broths used for pathogen screening in the food industry. The research spiked ten common Salmonella strains in leafy spinach greens and ground beef, to model "presumptive-positive" enriched samples, including multiple serotypes of Typhimurium and Enteritidis, as well as the Javiana, Newport, Montevideo and Heidelberg serotypes. Of the 240 samples spiked with Salmonella, GSS positively identified the correct Salmonella serotype in 235 of the samples, and no false positives were recorded from the 24 control samples. (Salmonella was not detected in five samples, due to poor growth of Salmonella in the enrichment of the initial sample, not related to the GSS technology.)

The GSS technology will be commercially available in 2014 in the RESOLUTION™ Microbial Genotyping System. PathoGenetix will exhibit the RESOLUTION System at IAFP from Sunday evening, July 28, through Tuesday, July 30, and will present its research in two poster sessions on Tuesday, July 30, and Wednesday, July 31. For more information on the RESOLUTION Microbia
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