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New England Biolabs Develops Novel Polymerase with Ultra-High Fidelity and Minimal GC Bias
Date:8/8/2012

ly reduced these limitations and raised the bar for high-fidelity DNA amplification," says Fiona Stewart, Ph.D., Product Marketing Manager at NEB.

A hot start version of the Q5 polymerase, which utilizes a synthetic aptamer for room-temperature set up, is also available. Both the Q5 and Q5 Hot Start DNA Polymerases are available as standalone enzymes or in 2X master mix format for added convenience. A separate formulation has also been developed specifically to meet the needs of amplification of next generation sequencing libraries (NEBNext® High-Fidelity 2X PCR Master Mix).

The discovery and development of high-fidelity polymerases has been, for many years, a key focus of NEB's research. Beginning with the discovery of Vent® DNA Polymerase from Thermococcus litoralis in 1990, and more recently the commercialization of Phusion® DNA Polymerase, NEB researchers have identified and developed a number of high-fidelity polymerases with differing processivities, speeds and degrees of accuracy. Q5 DNA Polymerase is the most recent and highest-performing enzyme to have been added to this list. For more information and supporting data on Q5 DNA Polymerase, visit www.Q5PCR.com.

About New England Biolabs


Established in the mid 1970's, New England Biolabs, Inc. is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, cellular analysis, epigenetics and RNA analysis. Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors. New England Biolabs is a privately held company, headquartered in Ipswich, MA and has extensive worldwide
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